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Title: HCV core protein induces hepatic lipid accumulation by activating SREBP1 and PPAR{gamma}

Abstract

Hepatic steatosis is a common feature in patients with chronic hepatitis C virus (HCV) infection. HCV core protein plays an important role in the development of hepatic steatosis in HCV infection. Because SREBP1 (sterol regulatory element binding protein 1) and PPAR{gamma} (peroxisome proliferators-activated receptor {gamma}) are involved in the regulation of lipid metabolism of hepatocyte, we sought to determine whether HCV core protein may impair the expression and activity of SREBP1 and PPAR{gamma}. In this study, it was demonstrated that HCV core protein increases the gene expression of SREBP1 not only in Chang liver, Huh7, and HepG2 cells transiently transfected with HCV core protein expression plasmid, but also in Chang liver-core stable cells. Furthermore, HCV core protein enhanced the transcriptional activity of SREBP1. In addition, HCV core protein elevated PPAR{gamma} transcriptional activity. However, HCV core protein had no effect on PPAR{gamma} gene expression. Finally, we showed that HCV core protein stimulates the genes expression of lipogenic enzyme and fatty acid uptake associated protein. Therefore, our finding provides a new insight into the mechanism of hepatic steatosis by HCV infection.

Authors:
 [1];  [1];  [1];  [1];  [2];  [3]
  1. Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 609-735 (Korea, Republic of)
  2. Department of Internal Medicine, Bongseng Memorial Hospital, Busan 601-723 (Korea, Republic of)
  3. Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 609-735 (Korea, Republic of). E-mail: molecule85@pusan.ac.kr
Publication Date:
OSTI Identifier:
20979881
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 355; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2007.02.044; PII: S0006-291X(07)00298-7; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CARBOXYLIC ACIDS; ENZYMES; GENE REGULATION; GENES; HEPATITIS; LIPIDS; LIVER; METABOLISM; PATIENTS; RECEPTORS; STEROLS; VIRUSES

Citation Formats

Kim, Kook Hwan, Hong, Sung Pyo, Kim, KyeongJin, Park, Min Jung, Kim, Kwang Jin, and Cheong, JaeHun. HCV core protein induces hepatic lipid accumulation by activating SREBP1 and PPAR{gamma}. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.02.044.
Kim, Kook Hwan, Hong, Sung Pyo, Kim, KyeongJin, Park, Min Jung, Kim, Kwang Jin, & Cheong, JaeHun. HCV core protein induces hepatic lipid accumulation by activating SREBP1 and PPAR{gamma}. United States. doi:10.1016/j.bbrc.2007.02.044.
Kim, Kook Hwan, Hong, Sung Pyo, Kim, KyeongJin, Park, Min Jung, Kim, Kwang Jin, and Cheong, JaeHun. Fri . "HCV core protein induces hepatic lipid accumulation by activating SREBP1 and PPAR{gamma}". United States. doi:10.1016/j.bbrc.2007.02.044.
@article{osti_20979881,
title = {HCV core protein induces hepatic lipid accumulation by activating SREBP1 and PPAR{gamma}},
author = {Kim, Kook Hwan and Hong, Sung Pyo and Kim, KyeongJin and Park, Min Jung and Kim, Kwang Jin and Cheong, JaeHun},
abstractNote = {Hepatic steatosis is a common feature in patients with chronic hepatitis C virus (HCV) infection. HCV core protein plays an important role in the development of hepatic steatosis in HCV infection. Because SREBP1 (sterol regulatory element binding protein 1) and PPAR{gamma} (peroxisome proliferators-activated receptor {gamma}) are involved in the regulation of lipid metabolism of hepatocyte, we sought to determine whether HCV core protein may impair the expression and activity of SREBP1 and PPAR{gamma}. In this study, it was demonstrated that HCV core protein increases the gene expression of SREBP1 not only in Chang liver, Huh7, and HepG2 cells transiently transfected with HCV core protein expression plasmid, but also in Chang liver-core stable cells. Furthermore, HCV core protein enhanced the transcriptional activity of SREBP1. In addition, HCV core protein elevated PPAR{gamma} transcriptional activity. However, HCV core protein had no effect on PPAR{gamma} gene expression. Finally, we showed that HCV core protein stimulates the genes expression of lipogenic enzyme and fatty acid uptake associated protein. Therefore, our finding provides a new insight into the mechanism of hepatic steatosis by HCV infection.},
doi = {10.1016/j.bbrc.2007.02.044},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 355,
place = {United States},
year = {Fri Apr 20 00:00:00 EDT 2007},
month = {Fri Apr 20 00:00:00 EDT 2007}
}