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Title: Downregulation of CCR1 inhibits human hepatocellular carcinoma cell invasion

Abstract

CC chemokine receptor 1 (CCR1) has an important role in the recruitment of leukocytes to the site of inflammation. The migration and metastasis of tumor cells shares many similarities with leukocyte trafficking, which is mainly regulated by chemokine receptor-ligand interactions. CCR1 is highly expressed in hepatocellular carcinoma (HCC) cells and tissues with unknown functions. In this study, we silenced CCR1 expression in the human HCC cell line HCCLM3 using artificial microRNA (miRNA)-mediated RNA interference (RNAi) and examined the invasiveness and proliferation of CCR1-silenced HCCLM3 cells and the matrix metalloproteinase (MMP) activity. The miRNA-mediated knockdown expression of CCR1 significantly inhibited the invasive ability of HCCLM3 cells, but had only a minor effect on the cellular proliferation rate. Moreover, CCR1 knockdown significantly reduced the secretion of MMP-2. Together, these findings indicate that CCR1 has an important role in HCCLM3 invasion and that CCR1 might be a new target of HCC treatment.

Authors:
 [1];  [1];  [2]; ;  [1];  [1];  [1];  [1];  [1];  [2];  [1];  [2]
  1. Liver Cancer Institute and Zhongshan Hospital, Fudan University, Shanghai 200032 (China)
  2. (China)
Publication Date:
OSTI Identifier:
20979879
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 355; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2007.01.199; PII: S0006-291X(07)00238-0; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; HEPATOMAS; HUMAN POPULATIONS; INFLAMMATION; LEUKOCYTES; LIGANDS; MATRICES; METASTASES; RECEPTORS; RNA; TUMOR CELLS

Citation Formats

Wu Xiaofeng, Fan Jia, Institute of Biomedical Sciences, Fudan University, Shanghai 200032, E-mail: jiafan99@yahoo.com, Wang Xiaoying, Zhou Jian, Qiu Shuangjian, Yu Yao, Liu Yinkun, Institute of Biomedical Sciences, Fudan University, Shanghai 200032, Tang Zhaoyou, and Institute of Biomedical Sciences, Fudan University, Shanghai 200032. Downregulation of CCR1 inhibits human hepatocellular carcinoma cell invasion. United States: N. p., 2007. Web.
Wu Xiaofeng, Fan Jia, Institute of Biomedical Sciences, Fudan University, Shanghai 200032, E-mail: jiafan99@yahoo.com, Wang Xiaoying, Zhou Jian, Qiu Shuangjian, Yu Yao, Liu Yinkun, Institute of Biomedical Sciences, Fudan University, Shanghai 200032, Tang Zhaoyou, & Institute of Biomedical Sciences, Fudan University, Shanghai 200032. Downregulation of CCR1 inhibits human hepatocellular carcinoma cell invasion. United States.
Wu Xiaofeng, Fan Jia, Institute of Biomedical Sciences, Fudan University, Shanghai 200032, E-mail: jiafan99@yahoo.com, Wang Xiaoying, Zhou Jian, Qiu Shuangjian, Yu Yao, Liu Yinkun, Institute of Biomedical Sciences, Fudan University, Shanghai 200032, Tang Zhaoyou, and Institute of Biomedical Sciences, Fudan University, Shanghai 200032. Fri . "Downregulation of CCR1 inhibits human hepatocellular carcinoma cell invasion". United States. doi:.
@article{osti_20979879,
title = {Downregulation of CCR1 inhibits human hepatocellular carcinoma cell invasion},
author = {Wu Xiaofeng and Fan Jia and Institute of Biomedical Sciences, Fudan University, Shanghai 200032 and E-mail: jiafan99@yahoo.com and Wang Xiaoying and Zhou Jian and Qiu Shuangjian and Yu Yao and Liu Yinkun and Institute of Biomedical Sciences, Fudan University, Shanghai 200032 and Tang Zhaoyou and Institute of Biomedical Sciences, Fudan University, Shanghai 200032},
abstractNote = {CC chemokine receptor 1 (CCR1) has an important role in the recruitment of leukocytes to the site of inflammation. The migration and metastasis of tumor cells shares many similarities with leukocyte trafficking, which is mainly regulated by chemokine receptor-ligand interactions. CCR1 is highly expressed in hepatocellular carcinoma (HCC) cells and tissues with unknown functions. In this study, we silenced CCR1 expression in the human HCC cell line HCCLM3 using artificial microRNA (miRNA)-mediated RNA interference (RNAi) and examined the invasiveness and proliferation of CCR1-silenced HCCLM3 cells and the matrix metalloproteinase (MMP) activity. The miRNA-mediated knockdown expression of CCR1 significantly inhibited the invasive ability of HCCLM3 cells, but had only a minor effect on the cellular proliferation rate. Moreover, CCR1 knockdown significantly reduced the secretion of MMP-2. Together, these findings indicate that CCR1 has an important role in HCCLM3 invasion and that CCR1 might be a new target of HCC treatment.},
doi = {},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 355,
place = {United States},
year = {Fri Apr 20 00:00:00 EDT 2007},
month = {Fri Apr 20 00:00:00 EDT 2007}
}