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Title: Single cell transfection using plasmid decorated AFM probes

Abstract

Eukaryotic cells were individually transfected using commercially available atomic force microscope tips decorated with plasmidic DNA encoding for the fluorescent protein EGFP. In a typical transfection attempt, the tip is forcibly incorporated into the cell thus allowing for the transfer of the genetic material through the cell membrane. A sharp discontinuity, corresponding to the passage of the tip through the cell membrane can be easily detected when monitoring the cellular deformation as a function of the applied force. In order for the transfection to be successful, the tip must reversibly penetrates the membrane without causing disturbance or damage to the cell. Transfection success rate (30%), cell survival, and growth are confirmed by epifluorescence microscopy. This technique provides an alternative tool to the transfection toolbox, allowing the transfection of specific individual cells with minimal disturbance.

Authors:
 [1];  [1];  [2]
  1. Departement de pharmacologie, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, Que., J1H 5N4 (Canada)
  2. Departement de pharmacologie, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, Que., J1H 5N4 (Canada). E-mail: michel.grandbois@usherbrooke.ca
Publication Date:
OSTI Identifier:
20979873
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 355; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2007.01.190; PII: S0006-291X(07)00246-X; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ATOMIC FORCE MICROSCOPY; CELL MEMBRANES; DNA; DNA DAMAGES; GROWTH; PROTEINS

Citation Formats

Cuerrier, Charles M., Lebel, Rejean, and Grandbois, Michel. Single cell transfection using plasmid decorated AFM probes. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.01.190.
Cuerrier, Charles M., Lebel, Rejean, & Grandbois, Michel. Single cell transfection using plasmid decorated AFM probes. United States. doi:10.1016/j.bbrc.2007.01.190.
Cuerrier, Charles M., Lebel, Rejean, and Grandbois, Michel. Fri . "Single cell transfection using plasmid decorated AFM probes". United States. doi:10.1016/j.bbrc.2007.01.190.
@article{osti_20979873,
title = {Single cell transfection using plasmid decorated AFM probes},
author = {Cuerrier, Charles M. and Lebel, Rejean and Grandbois, Michel},
abstractNote = {Eukaryotic cells were individually transfected using commercially available atomic force microscope tips decorated with plasmidic DNA encoding for the fluorescent protein EGFP. In a typical transfection attempt, the tip is forcibly incorporated into the cell thus allowing for the transfer of the genetic material through the cell membrane. A sharp discontinuity, corresponding to the passage of the tip through the cell membrane can be easily detected when monitoring the cellular deformation as a function of the applied force. In order for the transfection to be successful, the tip must reversibly penetrates the membrane without causing disturbance or damage to the cell. Transfection success rate (30%), cell survival, and growth are confirmed by epifluorescence microscopy. This technique provides an alternative tool to the transfection toolbox, allowing the transfection of specific individual cells with minimal disturbance.},
doi = {10.1016/j.bbrc.2007.01.190},
journal = {Biochemical and Biophysical Research Communications},
number = 3,
volume = 355,
place = {United States},
year = {Fri Apr 13 00:00:00 EDT 2007},
month = {Fri Apr 13 00:00:00 EDT 2007}
}