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Title: The expression and genetic immunization of chimeric fragment of Hantaan virus M and S segments

Abstract

Hemorrhagic fever with renal syndrome (HFRS), which is characterized by severe symptoms and high mortality, is caused by hantavirus. There are still no effective prophylactic vaccines directed to HFRS until now. In this research, we fused expressed G2 fragment of M segment and 0.7 kb fragment of S segment. We expect it could be a candidate vaccine. Chimeric gene G2S0.7 was first expressed in prokaryotic expression system pGEX-4T. After inducing expressed fusion proteins, GST-G2S0.7 was induced and its molecular weight was about 100 kDa. Meanwhile, the fusion protein kept the activity of its parental proteins. Further, BALB/c mice were vaccinated by the chimeric gene. ELISA, cell microculture neutralization test in vitro were used to detect the humoral immune response in immunized BALB/c mice. Lymphocyte proliferation assay was used to detect the cellular immune response. The results showed that the chimeric gene could simultaneously evoke specific antibody against nucleocapsid protein (NP) and glycoprotein (GP). And the immunized mice of every group elicited neutralizing antibodies with different titers. But the titers were low. Lymphocyte proliferation assay results showed that the stimulation indexes of splenocytes of chimeric gene to NP and GP were significantly higher than that of control. It suggested that themore » chimeric gene of Hantaan virus containing G2 fragment of M segment and 0.7 kb fragment of S segment could directly elicit specific anti-Hantaan virus humoral and cellular immune response in BALB/c mice.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2]
  1. Department of Microbiology, Fourth Military Medical University, Xi'an 710032 (China)
  2. Department of Microbiology, Fourth Military Medical University, Xi'an 710032 (China). E-mail: zhikaixu@fmmu.edu.cn
Publication Date:
OSTI Identifier:
20979836
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 354; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2007.01.020; PII: S0006-291X(07)00037-X; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; CELL PROLIFERATION; ENZYME IMMUNOASSAY; FEVER; GENES; GLYCOPROTEINS; IN VITRO; KIDNEYS; LYMPHOCYTES; MICE; MOLECULAR WEIGHT; MORTALITY; VACCINES; VIRUSES

Citation Formats

Zhang Fanglin, Wu Xingan, Luo Wen, Bai Wentao, Liu Yong, Yan Yan, Wang Haitao, and Xu Zhikai. The expression and genetic immunization of chimeric fragment of Hantaan virus M and S segments. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.01.020.
Zhang Fanglin, Wu Xingan, Luo Wen, Bai Wentao, Liu Yong, Yan Yan, Wang Haitao, & Xu Zhikai. The expression and genetic immunization of chimeric fragment of Hantaan virus M and S segments. United States. doi:10.1016/j.bbrc.2007.01.020.
Zhang Fanglin, Wu Xingan, Luo Wen, Bai Wentao, Liu Yong, Yan Yan, Wang Haitao, and Xu Zhikai. Fri . "The expression and genetic immunization of chimeric fragment of Hantaan virus M and S segments". United States. doi:10.1016/j.bbrc.2007.01.020.
@article{osti_20979836,
title = {The expression and genetic immunization of chimeric fragment of Hantaan virus M and S segments},
author = {Zhang Fanglin and Wu Xingan and Luo Wen and Bai Wentao and Liu Yong and Yan Yan and Wang Haitao and Xu Zhikai},
abstractNote = {Hemorrhagic fever with renal syndrome (HFRS), which is characterized by severe symptoms and high mortality, is caused by hantavirus. There are still no effective prophylactic vaccines directed to HFRS until now. In this research, we fused expressed G2 fragment of M segment and 0.7 kb fragment of S segment. We expect it could be a candidate vaccine. Chimeric gene G2S0.7 was first expressed in prokaryotic expression system pGEX-4T. After inducing expressed fusion proteins, GST-G2S0.7 was induced and its molecular weight was about 100 kDa. Meanwhile, the fusion protein kept the activity of its parental proteins. Further, BALB/c mice were vaccinated by the chimeric gene. ELISA, cell microculture neutralization test in vitro were used to detect the humoral immune response in immunized BALB/c mice. Lymphocyte proliferation assay was used to detect the cellular immune response. The results showed that the chimeric gene could simultaneously evoke specific antibody against nucleocapsid protein (NP) and glycoprotein (GP). And the immunized mice of every group elicited neutralizing antibodies with different titers. But the titers were low. Lymphocyte proliferation assay results showed that the stimulation indexes of splenocytes of chimeric gene to NP and GP were significantly higher than that of control. It suggested that the chimeric gene of Hantaan virus containing G2 fragment of M segment and 0.7 kb fragment of S segment could directly elicit specific anti-Hantaan virus humoral and cellular immune response in BALB/c mice.},
doi = {10.1016/j.bbrc.2007.01.020},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 354,
place = {United States},
year = {Fri Mar 23 00:00:00 EDT 2007},
month = {Fri Mar 23 00:00:00 EDT 2007}
}