skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: A component of green tea (-)-epigallocatechin-3-gallate, promotes apoptosis in T24 human bladder cancer cells via modulation of the PI3K/Akt pathway and Bcl-2 family proteins

Abstract

Bladder cancer is the fourth most common cancer in men and ninth most common in women. It has a protracted course of progression and is thus an ideal candidate for chemoprevention strategies and trials. This study was conducted to evaluate the chemopreventive/antiproliferative potential of (-)-epigallocatechin gallate (EGCG, the major phytochemical in green tea) against bladder cancer and its mechanism of action. Using the T24 human bladder cancer cell line, we found that EGCG treatment caused dose- and time-dependent inhibition of cellular proliferation and cell viability, and induced apoptosis. Mechanistically, EGCG inhibits phosphatidylinositol 3'-kinase/Akt activation that, in turn, results in modulation of Bcl-2 family proteins, leading to enhanced apoptosis of T24 cells. These findings suggest that EGCG may be an important chemoprevention agent for the management of bladder cancer.

Authors:
 [1];  [2];  [1];  [1];  [1];  [1];  [3];  [3]
  1. Department of Urology, First Affiliated Hospital, Medical College, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang Province (China)
  2. Department of Urology, First Affiliated Hospital, Medical College, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang Province (China). E-mail: xielp@zjuem.zju.edu.cn
  3. Department of Urology, University of California San Francisco and Veteran Affairs Medical Center San Francisco, San Francisco, CA (United States)
Publication Date:
OSTI Identifier:
20979835
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 354; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2007.01.003; PII: S0006-291X(07)00031-9; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; BEVERAGES; BLADDER; BORON CHLORIDES; CELL PROLIFERATION; GALLIUM COMPOUNDS; HUMAN POPULATIONS; INHIBITION; MITES; MODULATION; NEOPLASMS; OXYGEN COMPOUNDS; PROTEINS; RADIATION DOSES; TIME DEPENDENCE

Citation Formats

Qin Jie, Xie Liping, Zheng Xiangyi, Wang Yunbin, Bai Yu, Shen Huafeng, Li Longcheng, and Dahiya, Rajvir. A component of green tea (-)-epigallocatechin-3-gallate, promotes apoptosis in T24 human bladder cancer cells via modulation of the PI3K/Akt pathway and Bcl-2 family proteins. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.01.003.
Qin Jie, Xie Liping, Zheng Xiangyi, Wang Yunbin, Bai Yu, Shen Huafeng, Li Longcheng, & Dahiya, Rajvir. A component of green tea (-)-epigallocatechin-3-gallate, promotes apoptosis in T24 human bladder cancer cells via modulation of the PI3K/Akt pathway and Bcl-2 family proteins. United States. doi:10.1016/j.bbrc.2007.01.003.
Qin Jie, Xie Liping, Zheng Xiangyi, Wang Yunbin, Bai Yu, Shen Huafeng, Li Longcheng, and Dahiya, Rajvir. Fri . "A component of green tea (-)-epigallocatechin-3-gallate, promotes apoptosis in T24 human bladder cancer cells via modulation of the PI3K/Akt pathway and Bcl-2 family proteins". United States. doi:10.1016/j.bbrc.2007.01.003.
@article{osti_20979835,
title = {A component of green tea (-)-epigallocatechin-3-gallate, promotes apoptosis in T24 human bladder cancer cells via modulation of the PI3K/Akt pathway and Bcl-2 family proteins},
author = {Qin Jie and Xie Liping and Zheng Xiangyi and Wang Yunbin and Bai Yu and Shen Huafeng and Li Longcheng and Dahiya, Rajvir},
abstractNote = {Bladder cancer is the fourth most common cancer in men and ninth most common in women. It has a protracted course of progression and is thus an ideal candidate for chemoprevention strategies and trials. This study was conducted to evaluate the chemopreventive/antiproliferative potential of (-)-epigallocatechin gallate (EGCG, the major phytochemical in green tea) against bladder cancer and its mechanism of action. Using the T24 human bladder cancer cell line, we found that EGCG treatment caused dose- and time-dependent inhibition of cellular proliferation and cell viability, and induced apoptosis. Mechanistically, EGCG inhibits phosphatidylinositol 3'-kinase/Akt activation that, in turn, results in modulation of Bcl-2 family proteins, leading to enhanced apoptosis of T24 cells. These findings suggest that EGCG may be an important chemoprevention agent for the management of bladder cancer.},
doi = {10.1016/j.bbrc.2007.01.003},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 354,
place = {United States},
year = {Fri Mar 23 00:00:00 EDT 2007},
month = {Fri Mar 23 00:00:00 EDT 2007}
}