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Title: Role of microphthalmia transcription factor (Mitf) in melanoma differentiation

Abstract

We transfected the melanocyte-specific Mitf-M isoform into the aggressive melanoma UISO-Mel-6 cell lines. Our data show that Mitf decreases cell proliferation and results in cells which grow in clusters. By analyzing the expression of the markers of differentiation, we demonstrate that Mitf favored increased expression of tyrosinase and tyrosinase-related protein-1. In addition, Mitf induces Bcl-2 expression following transfection of UISO-Mel-6 cells. We also showed that Mitf gene affects cell-cycle distribution by resting cells preferentially in G2/G1 phase, and inducing the expression of p21 and p27. Moreover, we performed in vivo studies using subcutaneous injection of UISO-Mel-6 and UISO-Mel-6-Mitf in Balb/c nude mice. Our data show that Mitf inhibits tumor growth and decreases Ki67 expression. Tumors induced by UISO-Mel-6 cells were ulcerated and resulted in metastases to liver. None of the mice injected with UISO-Mel-6{sup Mitf+} cells harbored liver metastases. Our results suggest that Mitf is involved in melanoma differentiation and leads to a less aggressive phenotype.

Authors:
 [1];  [2];  [2];  [2];  [2]
  1. Department of Surgical Oncology, University of Illinois at Chicago, 840 South Wood Street, M/C 820, Chicago, IL 60612 (United States). E-mail: fatimal@uic.edu
  2. Department of Surgical Oncology, University of Illinois at Chicago, 840 South Wood Street, M/C 820, Chicago, IL 60612 (United States)
Publication Date:
OSTI Identifier:
20979834
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 354; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2007.01.075; PII: S0006-291X(07)00128-3; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BORON CHLORIDES; CELL CYCLE; CELL PROLIFERATION; GENES; LIVER; MELANOMAS; METASTASES; MICE; MONOCLINIC LATTICES; PHENOTYPE; SUBCUTANEOUS INJECTION; TRANSCRIPTION FACTORS; TYROSINASE

Citation Formats

Lekmine, Fatima, Chang, C.K., Sethakorn, Nan, Das Gupta, Tapas K., and Salti, George I. Role of microphthalmia transcription factor (Mitf) in melanoma differentiation. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.01.075.
Lekmine, Fatima, Chang, C.K., Sethakorn, Nan, Das Gupta, Tapas K., & Salti, George I. Role of microphthalmia transcription factor (Mitf) in melanoma differentiation. United States. doi:10.1016/j.bbrc.2007.01.075.
Lekmine, Fatima, Chang, C.K., Sethakorn, Nan, Das Gupta, Tapas K., and Salti, George I. Fri . "Role of microphthalmia transcription factor (Mitf) in melanoma differentiation". United States. doi:10.1016/j.bbrc.2007.01.075.
@article{osti_20979834,
title = {Role of microphthalmia transcription factor (Mitf) in melanoma differentiation},
author = {Lekmine, Fatima and Chang, C.K. and Sethakorn, Nan and Das Gupta, Tapas K. and Salti, George I.},
abstractNote = {We transfected the melanocyte-specific Mitf-M isoform into the aggressive melanoma UISO-Mel-6 cell lines. Our data show that Mitf decreases cell proliferation and results in cells which grow in clusters. By analyzing the expression of the markers of differentiation, we demonstrate that Mitf favored increased expression of tyrosinase and tyrosinase-related protein-1. In addition, Mitf induces Bcl-2 expression following transfection of UISO-Mel-6 cells. We also showed that Mitf gene affects cell-cycle distribution by resting cells preferentially in G2/G1 phase, and inducing the expression of p21 and p27. Moreover, we performed in vivo studies using subcutaneous injection of UISO-Mel-6 and UISO-Mel-6-Mitf in Balb/c nude mice. Our data show that Mitf inhibits tumor growth and decreases Ki67 expression. Tumors induced by UISO-Mel-6 cells were ulcerated and resulted in metastases to liver. None of the mice injected with UISO-Mel-6{sup Mitf+} cells harbored liver metastases. Our results suggest that Mitf is involved in melanoma differentiation and leads to a less aggressive phenotype.},
doi = {10.1016/j.bbrc.2007.01.075},
journal = {Biochemical and Biophysical Research Communications},
number = 3,
volume = 354,
place = {United States},
year = {Fri Mar 16 00:00:00 EDT 2007},
month = {Fri Mar 16 00:00:00 EDT 2007}
}