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Title: Identification of novel short peptides derived from the {alpha}4, {alpha}5, and {alpha}6 fibrils of type IV collagen with anti-angiogenic properties

Abstract

Angiogenesis, or neovascularization, is tightly controlled by positive and negative regulators, many of which reside in the extracellular matrix. We have now identified eight novel 19- to 20-residue peptides derived from the {alpha}4, {alpha}5, and {alpha}6 fibrils of type IV collagen, which we have designated tetrastatins, pentastatins, and hexastatins, respectively. We have shown that these endogenous peptides suppress the proliferation and migration of HUVECs in vitro. By performing clustering analyses of the sequences using sequence similarity criteria and of the experimental results using a hierarchical algorithm, we report that the clusters identified by the experimental results coincide with the sequence-based clusters, indicating a tight relationship between peptide sequence and anti-angiogenic potency. These peptides may have potential as anti-angiogenic therapeutic agents.

Authors:
 [1];  [2]
  1. Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205 (United States). E-mail: ekaragi1@jhmi.edu
  2. Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205 (United States)
Publication Date:
OSTI Identifier:
20979827
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 354; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2006.12.231; PII: S0006-291X(06)02889-0; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ALGORITHMS; AMINO ACID SEQUENCE; CELL PROLIFERATION; COLLAGEN; DRUGS; IN VITRO; PEPTIDES

Citation Formats

Karagiannis, Emmanouil D., and Popel, Aleksander S. Identification of novel short peptides derived from the {alpha}4, {alpha}5, and {alpha}6 fibrils of type IV collagen with anti-angiogenic properties. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2006.12.231.
Karagiannis, Emmanouil D., & Popel, Aleksander S. Identification of novel short peptides derived from the {alpha}4, {alpha}5, and {alpha}6 fibrils of type IV collagen with anti-angiogenic properties. United States. doi:10.1016/j.bbrc.2006.12.231.
Karagiannis, Emmanouil D., and Popel, Aleksander S. Fri . "Identification of novel short peptides derived from the {alpha}4, {alpha}5, and {alpha}6 fibrils of type IV collagen with anti-angiogenic properties". United States. doi:10.1016/j.bbrc.2006.12.231.
@article{osti_20979827,
title = {Identification of novel short peptides derived from the {alpha}4, {alpha}5, and {alpha}6 fibrils of type IV collagen with anti-angiogenic properties},
author = {Karagiannis, Emmanouil D. and Popel, Aleksander S.},
abstractNote = {Angiogenesis, or neovascularization, is tightly controlled by positive and negative regulators, many of which reside in the extracellular matrix. We have now identified eight novel 19- to 20-residue peptides derived from the {alpha}4, {alpha}5, and {alpha}6 fibrils of type IV collagen, which we have designated tetrastatins, pentastatins, and hexastatins, respectively. We have shown that these endogenous peptides suppress the proliferation and migration of HUVECs in vitro. By performing clustering analyses of the sequences using sequence similarity criteria and of the experimental results using a hierarchical algorithm, we report that the clusters identified by the experimental results coincide with the sequence-based clusters, indicating a tight relationship between peptide sequence and anti-angiogenic potency. These peptides may have potential as anti-angiogenic therapeutic agents.},
doi = {10.1016/j.bbrc.2006.12.231},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 354,
place = {United States},
year = {Fri Mar 09 00:00:00 EST 2007},
month = {Fri Mar 09 00:00:00 EST 2007}
}