skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Radiosensitization effect of zidovudine on human malignant glioma cells

Abstract

Telomeres are shortened with each cell division and play an important role in maintaining chromosomal integrity and function. Telomerase, responsible for telomere synthesis, is activated in 90% of human tumor cells but seldom in normal somatic cells. Zidovudine (AZT) is a reverse transcriptase inhibitor. In this study, we have investigated the effects of {gamma}-radiation in combination with AZT on telomerase activity (TA), telomere length, DNA single-strand breaks (SSBs), DNA double-strand breaks (DSBs), and the changes in radiosensitivity of human malignant glioma cell line U251. The results showed that the TA was suppressed by AZT but enhanced by irradiation, resulting in a deceleration of restored rate of shortened telomere, decreased repair rate of DNA strand breaks, and increased radiosensitivity of U251 cells. Our results suggested that telomerase activity and telomere length may serve as markers for estimating the efficacy of cancer radiotherapy and reverse transcriptase inhibitors, such as AZT, may be used clinically as a new radiosensitizer in cancer radiotherapy.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2]
  1. Department of Chemo-Radiotherapy Oncology, Zhongnan Hospital, Wuhan University, Cancer Center of Wuhan University, Wuhan, Hubei 430071 (China)
  2. Department of Chemo-Radiotherapy Oncology, Zhongnan Hospital, Wuhan University, Cancer Center of Wuhan University, Wuhan, Hubei 430071 (China). E-mail: yfzhouwhu@163.com
Publication Date:
OSTI Identifier:
20979822
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 354; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2006.12.180; PII: S0006-291X(06)02818-X; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BIOLOGICAL REPAIR; CELL DIVISION; DNA; GAMMA RADIATION; GLIOMAS; IRRADIATION; RADIOSENSITIVITY; RADIOTHERAPY; SOMATIC CELLS; STRAND BREAKS; TELOMERES; TUMOR CELLS

Citation Formats

Zhou Fuxiang, Liao Zhengkai, Dai Jing, Xiong Jie, Xie CongHua, Luo Zhiguo, Liu Shiquan, and Zhou Yunfeng. Radiosensitization effect of zidovudine on human malignant glioma cells. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2006.12.180.
Zhou Fuxiang, Liao Zhengkai, Dai Jing, Xiong Jie, Xie CongHua, Luo Zhiguo, Liu Shiquan, & Zhou Yunfeng. Radiosensitization effect of zidovudine on human malignant glioma cells. United States. doi:10.1016/j.bbrc.2006.12.180.
Zhou Fuxiang, Liao Zhengkai, Dai Jing, Xiong Jie, Xie CongHua, Luo Zhiguo, Liu Shiquan, and Zhou Yunfeng. Fri . "Radiosensitization effect of zidovudine on human malignant glioma cells". United States. doi:10.1016/j.bbrc.2006.12.180.
@article{osti_20979822,
title = {Radiosensitization effect of zidovudine on human malignant glioma cells},
author = {Zhou Fuxiang and Liao Zhengkai and Dai Jing and Xiong Jie and Xie CongHua and Luo Zhiguo and Liu Shiquan and Zhou Yunfeng},
abstractNote = {Telomeres are shortened with each cell division and play an important role in maintaining chromosomal integrity and function. Telomerase, responsible for telomere synthesis, is activated in 90% of human tumor cells but seldom in normal somatic cells. Zidovudine (AZT) is a reverse transcriptase inhibitor. In this study, we have investigated the effects of {gamma}-radiation in combination with AZT on telomerase activity (TA), telomere length, DNA single-strand breaks (SSBs), DNA double-strand breaks (DSBs), and the changes in radiosensitivity of human malignant glioma cell line U251. The results showed that the TA was suppressed by AZT but enhanced by irradiation, resulting in a deceleration of restored rate of shortened telomere, decreased repair rate of DNA strand breaks, and increased radiosensitivity of U251 cells. Our results suggested that telomerase activity and telomere length may serve as markers for estimating the efficacy of cancer radiotherapy and reverse transcriptase inhibitors, such as AZT, may be used clinically as a new radiosensitizer in cancer radiotherapy.},
doi = {10.1016/j.bbrc.2006.12.180},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 354,
place = {United States},
year = {Fri Mar 09 00:00:00 EST 2007},
month = {Fri Mar 09 00:00:00 EST 2007}
}