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Title: Characterization and functional analysis of a novel double-guide C/D box snoRNA in the fission yeast

Abstract

Ribose methylation of eukaryotic rRNA is directed by box C/D small nucleolar RNAs (snoRNAs), which pinpoint the nucleotide to be methylated in specific position within the rRNA sequence. Here, we report the identification of a novel double-guide C/D box snoRNA termed snR88 that directs methylation of two previously undetermined sites in 25S rRNA from the fission yeast. Knockout of the predicted TATA box of the snR88 gene resulted in the complete blocking of its expression, showing that snR88 is an independently transcribed gene and dispensable for yeast viability. The depletion of snR88 abolished 25S rRNA methylation at U2304 and U2497 simultaneously. Interestingly, an unusual pause of reverse transcription at U2495 was observed, which implies an unknown structure of 25S rRNA related to ribose methylation at U2497 in the fission yeast.

Authors:
 [1];  [1];  [2]
  1. Key Laboratory of Gene Engineering of the Ministry of Education, Biotechnology Research Center, Zhongshan University, Guangzhou 510275 (China)
  2. Key Laboratory of Gene Engineering of the Ministry of Education, Biotechnology Research Center, Zhongshan University, Guangzhou 510275 (China). E-mail: lsszh@zsu.edu.cn
Publication Date:
OSTI Identifier:
20979821
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 354; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2006.12.207; PII: S0006-291X(07)00009-5; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; FUNCTIONAL ANALYSIS; GENES; KNOCK-OUT REACTIONS; METHYLATION; NUCLEOTIDES; RIBOSE; RNA; TRANSCRIPTION; YEASTS

Citation Formats

Bi Yanzhen, Qu Lianghu, and Zhou Hui. Characterization and functional analysis of a novel double-guide C/D box snoRNA in the fission yeast. United States: N. p., 2007. Web.
Bi Yanzhen, Qu Lianghu, & Zhou Hui. Characterization and functional analysis of a novel double-guide C/D box snoRNA in the fission yeast. United States.
Bi Yanzhen, Qu Lianghu, and Zhou Hui. Fri . "Characterization and functional analysis of a novel double-guide C/D box snoRNA in the fission yeast". United States. doi:.
@article{osti_20979821,
title = {Characterization and functional analysis of a novel double-guide C/D box snoRNA in the fission yeast},
author = {Bi Yanzhen and Qu Lianghu and Zhou Hui},
abstractNote = {Ribose methylation of eukaryotic rRNA is directed by box C/D small nucleolar RNAs (snoRNAs), which pinpoint the nucleotide to be methylated in specific position within the rRNA sequence. Here, we report the identification of a novel double-guide C/D box snoRNA termed snR88 that directs methylation of two previously undetermined sites in 25S rRNA from the fission yeast. Knockout of the predicted TATA box of the snR88 gene resulted in the complete blocking of its expression, showing that snR88 is an independently transcribed gene and dispensable for yeast viability. The depletion of snR88 abolished 25S rRNA methylation at U2304 and U2497 simultaneously. Interestingly, an unusual pause of reverse transcription at U2495 was observed, which implies an unknown structure of 25S rRNA related to ribose methylation at U2497 in the fission yeast.},
doi = {},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 354,
place = {United States},
year = {Fri Mar 02 00:00:00 EST 2007},
month = {Fri Mar 02 00:00:00 EST 2007}
}