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Title: Mtf-1 lymphoma-susceptibility locus affects retention of large thymocytes with high ROS levels in mice after {gamma}-irradiation

Abstract

Mouse strains exhibit different susceptibilities to {gamma}-ray-induced thymic lymphomas. Our previous study identified Mtf-1 (metal responsive transcription factor-1) as a candidate susceptibility gene, which is involved in the radiation-induced signaling pathway that regulates the cellular reactive oxygen species (ROS). To reveal the mechanism for the increased susceptibility conferred by Mtf-1 locus, we examined early effects of {gamma}-ray on ROS levels in vivo and its difference between Mtf-1 susceptible and resistant congenic mice. Here, we show the detection of clonally growing thymocytes at 4 weeks after irradiation, indicating the start of clonal expansion at a very early stage. We also show that large thymocytes with higher ROS levels and a proliferation capacity were more numerous in the Mtf-1 susceptible mice than the resistant mice when examined at 7 days after irradiation, although such tendency was not found in mice lacking one allele of Bcl11b tumor suppressor gene. This high retention of the large thymocytes, at a high risk for ROS-induced mutation, is a compensatory proliferation and regeneration response to depletion of the thymocytes after irradiation and the response is likely to augment the development of prelymphoma cells leading to thymic lymphomas.

Authors:
 [1];  [2];  [1];  [1];  [1];  [1];  [2];  [3];  [1];  [2];
  1. Department of Molecular Genetics, Niigata University Graduate School of Medical and Dental Sciences, Asahimachi 1-757, Niigata 951-8510 (Japan)
  2. (Japan)
  3. Third Department of Internal Medicine, Niigata University School of Medicine, Asahimachi 1-757, Niigata 951-8510 (Japan)
Publication Date:
OSTI Identifier:
20979818
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 354; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2006.12.192; PII: S0006-291X(06)02862-2; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; CELL PROLIFERATION; GAMMA RADIATION; IRRADIATION; LYMPHOMAS; MICE; THYMOCYTES; TRANSCRIPTION FACTORS

Citation Formats

Maruyama, Masaki, Third Department of Internal Medicine, Niigata University School of Medicine, Asahimachi 1-757, Niigata 951-8510, Yamamoto, Takashi, Kohara, Yuki, Katsuragi, Yoshinori, Mishima, Yukio, Transdisciplinary Research, Niigata University, Asahimachi 1-757, Niigata 951-8510, Aoyagi, Yutaka, Kominami, Ryo, Transdisciplinary Research, Niigata University, Asahimachi 1-757, Niigata 951-8510, and E-mail: rykomina@med.niigata-u.ac.jp. Mtf-1 lymphoma-susceptibility locus affects retention of large thymocytes with high ROS levels in mice after {gamma}-irradiation. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2006.12.192.
Maruyama, Masaki, Third Department of Internal Medicine, Niigata University School of Medicine, Asahimachi 1-757, Niigata 951-8510, Yamamoto, Takashi, Kohara, Yuki, Katsuragi, Yoshinori, Mishima, Yukio, Transdisciplinary Research, Niigata University, Asahimachi 1-757, Niigata 951-8510, Aoyagi, Yutaka, Kominami, Ryo, Transdisciplinary Research, Niigata University, Asahimachi 1-757, Niigata 951-8510, & E-mail: rykomina@med.niigata-u.ac.jp. Mtf-1 lymphoma-susceptibility locus affects retention of large thymocytes with high ROS levels in mice after {gamma}-irradiation. United States. doi:10.1016/j.bbrc.2006.12.192.
Maruyama, Masaki, Third Department of Internal Medicine, Niigata University School of Medicine, Asahimachi 1-757, Niigata 951-8510, Yamamoto, Takashi, Kohara, Yuki, Katsuragi, Yoshinori, Mishima, Yukio, Transdisciplinary Research, Niigata University, Asahimachi 1-757, Niigata 951-8510, Aoyagi, Yutaka, Kominami, Ryo, Transdisciplinary Research, Niigata University, Asahimachi 1-757, Niigata 951-8510, and E-mail: rykomina@med.niigata-u.ac.jp. Fri . "Mtf-1 lymphoma-susceptibility locus affects retention of large thymocytes with high ROS levels in mice after {gamma}-irradiation". United States. doi:10.1016/j.bbrc.2006.12.192.
@article{osti_20979818,
title = {Mtf-1 lymphoma-susceptibility locus affects retention of large thymocytes with high ROS levels in mice after {gamma}-irradiation},
author = {Maruyama, Masaki and Third Department of Internal Medicine, Niigata University School of Medicine, Asahimachi 1-757, Niigata 951-8510 and Yamamoto, Takashi and Kohara, Yuki and Katsuragi, Yoshinori and Mishima, Yukio and Transdisciplinary Research, Niigata University, Asahimachi 1-757, Niigata 951-8510 and Aoyagi, Yutaka and Kominami, Ryo and Transdisciplinary Research, Niigata University, Asahimachi 1-757, Niigata 951-8510 and E-mail: rykomina@med.niigata-u.ac.jp},
abstractNote = {Mouse strains exhibit different susceptibilities to {gamma}-ray-induced thymic lymphomas. Our previous study identified Mtf-1 (metal responsive transcription factor-1) as a candidate susceptibility gene, which is involved in the radiation-induced signaling pathway that regulates the cellular reactive oxygen species (ROS). To reveal the mechanism for the increased susceptibility conferred by Mtf-1 locus, we examined early effects of {gamma}-ray on ROS levels in vivo and its difference between Mtf-1 susceptible and resistant congenic mice. Here, we show the detection of clonally growing thymocytes at 4 weeks after irradiation, indicating the start of clonal expansion at a very early stage. We also show that large thymocytes with higher ROS levels and a proliferation capacity were more numerous in the Mtf-1 susceptible mice than the resistant mice when examined at 7 days after irradiation, although such tendency was not found in mice lacking one allele of Bcl11b tumor suppressor gene. This high retention of the large thymocytes, at a high risk for ROS-induced mutation, is a compensatory proliferation and regeneration response to depletion of the thymocytes after irradiation and the response is likely to augment the development of prelymphoma cells leading to thymic lymphomas.},
doi = {10.1016/j.bbrc.2006.12.192},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 354,
place = {United States},
year = {Fri Mar 02 00:00:00 EST 2007},
month = {Fri Mar 02 00:00:00 EST 2007}
}