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Title: Carbamylated erythropoietin protects the kidneys from ischemia-reperfusion injury without stimulating erythropoiesis

Abstract

Several studies have shown that erythropoietin (EPO) can protect the kidneys from ischemia-reperfusion injury and can raise the hemoglobin (Hb) concentration. Recently, the EPO molecule modified by carbamylation (CEPO) has been identified and was demonstrated to be able to protect several organs without increasing the Hb concentration. We hypothesized that treatment with CEPO would protect the kidneys from tubular apoptosis and inhibit subsequent tubulointerstitial injury without erythropoiesis. The therapeutic effect of CEPO was evaluated using a rat ischemia-reperfusion injury model. Saline-treated kidneys exhibited increased tubular apoptosis with interstitial expression of {alpha}-smooth muscle actin ({alpha}-SMA), while EPO treatment inhibited tubular apoptosis and {alpha}-SMA expression to some extent. On the other hand, CEPO-treated kidneys showed minimal tubular apoptosis with limited expression of {alpha}-SMA. Moreover, CEPO significantly promoted tubular epithelial cell proliferation without erythropoiesis. In conclusion, we identified a new therapeutic approach using CEPO to protect kidneys from ischemia-reperfusion injury.

Authors:
 [1];  [2];  [1];  [3];  [1]
  1. Department of Urology, Osaka University Graduate School of Medicine, Suita (Japan)
  2. Department of Advanced Technology for Transplantation, Osaka University Graduate School of Medicine, Suita (Japan). E-mail: isaka@att.med.osaka-u.ac.jp
  3. Department of Advanced Technology for Transplantation, Osaka University Graduate School of Medicine, Suita (Japan)
Publication Date:
OSTI Identifier:
20979801
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 353; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2006.12.099; PII: S0006-291X(06)02771-9; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACTIN; APOPTOSIS; CELL PROLIFERATION; ERYTHROPOIESIS; ERYTHROPOIETIN; HEMOGLOBIN; INJURIES; ISCHEMIA; KIDNEYS; MOLECULES; MUSCLES; RATS

Citation Formats

Imamura, Ryoichi, Isaka, Yoshitaka, Ichimaru, Naotsugu, Takahara, Shiro, and Okuyama, Akihiko. Carbamylated erythropoietin protects the kidneys from ischemia-reperfusion injury without stimulating erythropoiesis. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2006.12.099.
Imamura, Ryoichi, Isaka, Yoshitaka, Ichimaru, Naotsugu, Takahara, Shiro, & Okuyama, Akihiko. Carbamylated erythropoietin protects the kidneys from ischemia-reperfusion injury without stimulating erythropoiesis. United States. doi:10.1016/j.bbrc.2006.12.099.
Imamura, Ryoichi, Isaka, Yoshitaka, Ichimaru, Naotsugu, Takahara, Shiro, and Okuyama, Akihiko. Fri . "Carbamylated erythropoietin protects the kidneys from ischemia-reperfusion injury without stimulating erythropoiesis". United States. doi:10.1016/j.bbrc.2006.12.099.
@article{osti_20979801,
title = {Carbamylated erythropoietin protects the kidneys from ischemia-reperfusion injury without stimulating erythropoiesis},
author = {Imamura, Ryoichi and Isaka, Yoshitaka and Ichimaru, Naotsugu and Takahara, Shiro and Okuyama, Akihiko},
abstractNote = {Several studies have shown that erythropoietin (EPO) can protect the kidneys from ischemia-reperfusion injury and can raise the hemoglobin (Hb) concentration. Recently, the EPO molecule modified by carbamylation (CEPO) has been identified and was demonstrated to be able to protect several organs without increasing the Hb concentration. We hypothesized that treatment with CEPO would protect the kidneys from tubular apoptosis and inhibit subsequent tubulointerstitial injury without erythropoiesis. The therapeutic effect of CEPO was evaluated using a rat ischemia-reperfusion injury model. Saline-treated kidneys exhibited increased tubular apoptosis with interstitial expression of {alpha}-smooth muscle actin ({alpha}-SMA), while EPO treatment inhibited tubular apoptosis and {alpha}-SMA expression to some extent. On the other hand, CEPO-treated kidneys showed minimal tubular apoptosis with limited expression of {alpha}-SMA. Moreover, CEPO significantly promoted tubular epithelial cell proliferation without erythropoiesis. In conclusion, we identified a new therapeutic approach using CEPO to protect kidneys from ischemia-reperfusion injury.},
doi = {10.1016/j.bbrc.2006.12.099},
journal = {Biochemical and Biophysical Research Communications},
number = 3,
volume = 353,
place = {United States},
year = {Fri Feb 16 00:00:00 EST 2007},
month = {Fri Feb 16 00:00:00 EST 2007}
}