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Title: {beta}-Catenin up-regulates Nanog expression through interaction with Oct-3/4 in embryonic stem cells

Abstract

It is well known that mouse embryonic stem (ES) cells can be maintained by the presence of leukemia inhibitory factor (LIF). Recent studies have revealed that Wnt also exhibits activity similar to LIF. The molecular mechanism behind the maintenance of ES cells by these factors, however, is not fully understood. In this study, we found that LIF enhances level of nuclear {beta}-catenin, a component of the Wnt signaling pathway. Expression of an activated mutant of {beta}-catenin led to the long-term proliferation of ES cells, even in the absence of LIF. Furthermore, it was found that {beta}-catenin up-regulates Nanog in an Oct-3/4-dependent manner and that {beta}-catenin physically associates with Oct-3/4. These results suggest that up-regulating Nanog through interaction with Oct-3/4 involves {beta}-catenin in the LIF- and Wnt-mediated maintenance of ES cell self-renewal.

Authors:
 [1];  [1];  [2]
  1. Department of Stem Cell Biology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa 920-8640 (Japan)
  2. Department of Stem Cell Biology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa 920-8640 (Japan). E-mail: hkoide@med.kanazawa-u.ac.jp
Publication Date:
OSTI Identifier:
20979798
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 353; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2006.12.072; PII: S0006-291X(06)02748-3; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; LEUKEMIA; LITHIUM FLUORIDES; MICE; MUTANTS; STEM CELLS

Citation Formats

Takao, Yukinari, Yokota, Takashi, and Koide, Hiroshi. {beta}-Catenin up-regulates Nanog expression through interaction with Oct-3/4 in embryonic stem cells. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2006.12.072.
Takao, Yukinari, Yokota, Takashi, & Koide, Hiroshi. {beta}-Catenin up-regulates Nanog expression through interaction with Oct-3/4 in embryonic stem cells. United States. doi:10.1016/j.bbrc.2006.12.072.
Takao, Yukinari, Yokota, Takashi, and Koide, Hiroshi. Fri . "{beta}-Catenin up-regulates Nanog expression through interaction with Oct-3/4 in embryonic stem cells". United States. doi:10.1016/j.bbrc.2006.12.072.
@article{osti_20979798,
title = {{beta}-Catenin up-regulates Nanog expression through interaction with Oct-3/4 in embryonic stem cells},
author = {Takao, Yukinari and Yokota, Takashi and Koide, Hiroshi},
abstractNote = {It is well known that mouse embryonic stem (ES) cells can be maintained by the presence of leukemia inhibitory factor (LIF). Recent studies have revealed that Wnt also exhibits activity similar to LIF. The molecular mechanism behind the maintenance of ES cells by these factors, however, is not fully understood. In this study, we found that LIF enhances level of nuclear {beta}-catenin, a component of the Wnt signaling pathway. Expression of an activated mutant of {beta}-catenin led to the long-term proliferation of ES cells, even in the absence of LIF. Furthermore, it was found that {beta}-catenin up-regulates Nanog in an Oct-3/4-dependent manner and that {beta}-catenin physically associates with Oct-3/4. These results suggest that up-regulating Nanog through interaction with Oct-3/4 involves {beta}-catenin in the LIF- and Wnt-mediated maintenance of ES cell self-renewal.},
doi = {10.1016/j.bbrc.2006.12.072},
journal = {Biochemical and Biophysical Research Communications},
number = 3,
volume = 353,
place = {United States},
year = {Fri Feb 16 00:00:00 EST 2007},
month = {Fri Feb 16 00:00:00 EST 2007}
}