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Title: Identification of a cytoplasmic interaction partner of the large regulatory proteins Rep78/Rep68 of adeno-associated virus type 2 (AAV-2)

Abstract

Through yeast two-hybrid analysis and coimmunoprecipitation studies, we have identified a novel cellular AAV-2 Rep78/Rep68 interaction partner located predominantly in the cytoplasm. In public databases, it has been assigned as KCTD5, because of a region of high similarity to the cytoplasmic tetramerization domain of voltage-gated potassium channels. Whereas Rep/KCTD5 interaction relied on the region surrounding the Rep nuclear localization signal, nuclear accumulation of Rep was not required. Wildtype Rep78/Rep68 proteins induced the translocation of large portions of KCTD5 into the nucleus pointing to functional interactions both in the cytoplasm and the nucleus. In line with an anticipated functional interference in the cytoplasm, KCTD5 overexpression completely abrogated Rep68-mediated posttranscriptional activation of a HIV-LTR driven luciferase reporter gene. Our study expands the panel of already identified nuclear Rep interaction partners to a cytoplasmic protein, which raises the awareness that important steps in the AAV life cycle may be regulated in this compartment.

Authors:
 [1];  [2];  [2];  [2]
  1. Institut fuer Virologie, Charite Campus Benjamin Franklin, Freie Universitaet Berlin, Hindenburgdamm 27, 12203 Berlin (Germany). E-mail: stefan.weger@charite.de
  2. Institut fuer Virologie, Charite Campus Benjamin Franklin, Freie Universitaet Berlin, Hindenburgdamm 27, 12203 Berlin (Germany)
Publication Date:
OSTI Identifier:
20977028
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 362; Journal Issue: 1; Other Information: DOI: 10.1016/j.virol.2006.12.010; PII: S0042-6822(06)00892-0; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AIDS VIRUS; CYTOPLASM; ELECTRIC POTENTIAL; GENES; HYBRIDIZATION; LIFE CYCLE; LUCIFERASE; POTASSIUM; TRANSLOCATION; YEASTS

Citation Formats

Weger, Stefan, Hammer, Eva, Goetz, Anne, and Heilbronn, Regine. Identification of a cytoplasmic interaction partner of the large regulatory proteins Rep78/Rep68 of adeno-associated virus type 2 (AAV-2). United States: N. p., 2007. Web. doi:10.1016/j.virol.2006.12.010.
Weger, Stefan, Hammer, Eva, Goetz, Anne, & Heilbronn, Regine. Identification of a cytoplasmic interaction partner of the large regulatory proteins Rep78/Rep68 of adeno-associated virus type 2 (AAV-2). United States. doi:10.1016/j.virol.2006.12.010.
Weger, Stefan, Hammer, Eva, Goetz, Anne, and Heilbronn, Regine. Fri . "Identification of a cytoplasmic interaction partner of the large regulatory proteins Rep78/Rep68 of adeno-associated virus type 2 (AAV-2)". United States. doi:10.1016/j.virol.2006.12.010.
@article{osti_20977028,
title = {Identification of a cytoplasmic interaction partner of the large regulatory proteins Rep78/Rep68 of adeno-associated virus type 2 (AAV-2)},
author = {Weger, Stefan and Hammer, Eva and Goetz, Anne and Heilbronn, Regine},
abstractNote = {Through yeast two-hybrid analysis and coimmunoprecipitation studies, we have identified a novel cellular AAV-2 Rep78/Rep68 interaction partner located predominantly in the cytoplasm. In public databases, it has been assigned as KCTD5, because of a region of high similarity to the cytoplasmic tetramerization domain of voltage-gated potassium channels. Whereas Rep/KCTD5 interaction relied on the region surrounding the Rep nuclear localization signal, nuclear accumulation of Rep was not required. Wildtype Rep78/Rep68 proteins induced the translocation of large portions of KCTD5 into the nucleus pointing to functional interactions both in the cytoplasm and the nucleus. In line with an anticipated functional interference in the cytoplasm, KCTD5 overexpression completely abrogated Rep68-mediated posttranscriptional activation of a HIV-LTR driven luciferase reporter gene. Our study expands the panel of already identified nuclear Rep interaction partners to a cytoplasmic protein, which raises the awareness that important steps in the AAV life cycle may be regulated in this compartment.},
doi = {10.1016/j.virol.2006.12.010},
journal = {Virology},
number = 1,
volume = 362,
place = {United States},
year = {Fri May 25 00:00:00 EDT 2007},
month = {Fri May 25 00:00:00 EDT 2007}
}