skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Retro-transduction by virus pseudotyped with glycoprotein of vesicular stomatitis virus

Abstract

A virus pseudotyped with glycoprotein of vesicular stomatitis virus (VSV-G) can enter various cell types at a relatively high titer. We observed that the amount of viral antigen from VSV-G pseudotyped human immunodeficiency virus type 1 (HIV-1) producing cells was much higher than that from their non-pseudotyped counterparts. This enhanced viral antigen production was not observed when we used HIV-1 pol mutant, viral enzyme inhibitors, HIV Env protein, or VSV-G fusion defective mutants. The transfection experiment using GFP-expressing virus showed time-dependent expansion of GFP-positive cells and viral DNA integration. These results suggested that the increase in viral antigen yield was caused by the release of a progeny virus following retro-transduction by the pseudotyped virus of the cells within the transfected cell culture. The infectivity as well as the amount of VSV-G on virus particles per unit of viral antigen was significantly different before and after the onset of the yield enhancement. This suggests that results of infection assays of the virus pseudotyped with VSV-G may be affected by the occurrence of such enhancement. This means that, while pseudotyping with VSV-G is a simple and effective method, this procedure should be carefully considered when the virus is produced for infectivity assays.

Authors:
 [1];  [1];  [2]
  1. Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita City, Osaka 565-0871 (Japan)
  2. Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita City, Osaka 565-0871 (Japan). E-mail: sakuragi@biken.osaka-u.ac.jp
Publication Date:
OSTI Identifier:
20977026
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 362; Journal Issue: 1; Other Information: DOI: 10.1016/j.virol.2006.12.030; PII: S0042-6822(06)00939-1; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AIDS VIRUS; ANTIGENS; CELL CULTURES; DNA; ENZYME INHIBITORS; GLYCOPROTEINS; INFECTIVITY; MUTANTS; TIME DEPENDENCE

Citation Formats

Ohishi, Masahisa, Shioda, Tatsuo, and Sakuragi, Jun-ichi. Retro-transduction by virus pseudotyped with glycoprotein of vesicular stomatitis virus. United States: N. p., 2007. Web. doi:10.1016/j.virol.2006.12.030.
Ohishi, Masahisa, Shioda, Tatsuo, & Sakuragi, Jun-ichi. Retro-transduction by virus pseudotyped with glycoprotein of vesicular stomatitis virus. United States. doi:10.1016/j.virol.2006.12.030.
Ohishi, Masahisa, Shioda, Tatsuo, and Sakuragi, Jun-ichi. Fri . "Retro-transduction by virus pseudotyped with glycoprotein of vesicular stomatitis virus". United States. doi:10.1016/j.virol.2006.12.030.
@article{osti_20977026,
title = {Retro-transduction by virus pseudotyped with glycoprotein of vesicular stomatitis virus},
author = {Ohishi, Masahisa and Shioda, Tatsuo and Sakuragi, Jun-ichi},
abstractNote = {A virus pseudotyped with glycoprotein of vesicular stomatitis virus (VSV-G) can enter various cell types at a relatively high titer. We observed that the amount of viral antigen from VSV-G pseudotyped human immunodeficiency virus type 1 (HIV-1) producing cells was much higher than that from their non-pseudotyped counterparts. This enhanced viral antigen production was not observed when we used HIV-1 pol mutant, viral enzyme inhibitors, HIV Env protein, or VSV-G fusion defective mutants. The transfection experiment using GFP-expressing virus showed time-dependent expansion of GFP-positive cells and viral DNA integration. These results suggested that the increase in viral antigen yield was caused by the release of a progeny virus following retro-transduction by the pseudotyped virus of the cells within the transfected cell culture. The infectivity as well as the amount of VSV-G on virus particles per unit of viral antigen was significantly different before and after the onset of the yield enhancement. This suggests that results of infection assays of the virus pseudotyped with VSV-G may be affected by the occurrence of such enhancement. This means that, while pseudotyping with VSV-G is a simple and effective method, this procedure should be carefully considered when the virus is produced for infectivity assays.},
doi = {10.1016/j.virol.2006.12.030},
journal = {Virology},
number = 1,
volume = 362,
place = {United States},
year = {Fri May 25 00:00:00 EDT 2007},
month = {Fri May 25 00:00:00 EDT 2007}
}