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Title: Herpes simplex virus type 1 tegument proteins VP1/2 and UL37 are associated with intranuclear capsids

Abstract

The assembly of the tegument of herpes simplex virus type 1 (HSV-1) is a complex process that involves a number of events at various sites within virus-infected cells. Our studies focused on determining whether tegument proteins, VP1/2 and UL37, are added to capsids located within the nucleus. Capsids were isolated from the nuclear fraction of HSV-1-infected cells and purified by rate-zonal centrifugation to separate B capsids (containing the scaffold proteins and no viral DNA) and C capsids (containing DNA and no scaffold proteins). Western blot analyses of these capsids indicated that VP1/2 associated primarily with C capsids and UL37 associated with B and C capsids. The results demonstrate that at least two of the tegument proteins of HSV-1 are associated with capsids isolated from the nuclear fraction, and these capsid-tegument protein interactions may represent initial events of the tegumentation process.

Authors:
 [1];  [1];  [1];  [1];  [2]
  1. Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033 (United States)
  2. Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033 (United States). E-mail: rcourtney@psu.edu
Publication Date:
OSTI Identifier:
20977020
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 361; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2006.11.031; PII: S0042-6822(06)00880-4; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL NUCLEI; CENTRIFUGATION; DNA; HERPES SIMPLEX; PROTEINS; VIRUSES

Citation Formats

Bucks, Michelle A., O'Regan, Kevin J., Murphy, Michael A., Wills, John W., and Courtney, Richard J. Herpes simplex virus type 1 tegument proteins VP1/2 and UL37 are associated with intranuclear capsids. United States: N. p., 2007. Web. doi:10.1016/j.virol.2006.11.031.
Bucks, Michelle A., O'Regan, Kevin J., Murphy, Michael A., Wills, John W., & Courtney, Richard J. Herpes simplex virus type 1 tegument proteins VP1/2 and UL37 are associated with intranuclear capsids. United States. doi:10.1016/j.virol.2006.11.031.
Bucks, Michelle A., O'Regan, Kevin J., Murphy, Michael A., Wills, John W., and Courtney, Richard J. Thu . "Herpes simplex virus type 1 tegument proteins VP1/2 and UL37 are associated with intranuclear capsids". United States. doi:10.1016/j.virol.2006.11.031.
@article{osti_20977020,
title = {Herpes simplex virus type 1 tegument proteins VP1/2 and UL37 are associated with intranuclear capsids},
author = {Bucks, Michelle A. and O'Regan, Kevin J. and Murphy, Michael A. and Wills, John W. and Courtney, Richard J.},
abstractNote = {The assembly of the tegument of herpes simplex virus type 1 (HSV-1) is a complex process that involves a number of events at various sites within virus-infected cells. Our studies focused on determining whether tegument proteins, VP1/2 and UL37, are added to capsids located within the nucleus. Capsids were isolated from the nuclear fraction of HSV-1-infected cells and purified by rate-zonal centrifugation to separate B capsids (containing the scaffold proteins and no viral DNA) and C capsids (containing DNA and no scaffold proteins). Western blot analyses of these capsids indicated that VP1/2 associated primarily with C capsids and UL37 associated with B and C capsids. The results demonstrate that at least two of the tegument proteins of HSV-1 are associated with capsids isolated from the nuclear fraction, and these capsid-tegument protein interactions may represent initial events of the tegumentation process.},
doi = {10.1016/j.virol.2006.11.031},
journal = {Virology},
number = 2,
volume = 361,
place = {United States},
year = {Thu May 10 00:00:00 EDT 2007},
month = {Thu May 10 00:00:00 EDT 2007}
}