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Title: Selection of SARS-Coronavirus-specific B cell epitopes by phage peptide library screening and evaluation of the immunological effect of epitope-based peptides on mice

Abstract

Antibodies to SARS-Coronavirus (SARS-CoV)-specific B cell epitopes might recognize the pathogen and interrupt its adherence to and penetration of host cells. Hence, these epitopes could be useful for diagnosis and as vaccine constituents. Using the phage-displayed peptide library screening method and purified Fab fragments of immunoglobulin G (IgG Fab) from normal human sera and convalescent sera from SARS-CoV-infected patients as targets, 11 B cell epitopes of SARS-CoV spike glycoprotein (S protein) and membrane protein (M protein) were screened. After a bioinformatics tool was used to analyze these epitopes, four epitope-based S protein dodecapeptides corresponding to the predominant epitopes were chosen for synthesis. Their antigenic specificities and immunogenicities were studied in vitro and in vivo. Flow cytometry and ELISPOT analysis of lymphocytes as well as a serologic analysis of antibody showed that these peptides could trigger a rapid, highly effective, and relatively safe immune response in BALB/c mice. These findings might aid development of SARS diagnostics and vaccines. Moreover, the role of S and M proteins as important surface antigens is confirmed.

Authors:
 [1];  [2];  [1];  [1];  [1];  [1];  [1];  [3];  [4];  [4]
  1. Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080 (China)
  2. Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080 (China). E-mail: jianglf909@yahoo.com.cn
  3. Guangzhou City CDC (China)
  4. Department of Microbiology, Southern Medical University, Guangzhou (China)
Publication Date:
OSTI Identifier:
20976998
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 359; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2006.09.016; PII: S0042-6822(06)00669-6; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; ANTIGENS; BACTERIOPHAGES; BIOSYNTHESIS; DIAGNOSIS; EVALUATION; GLYCOPROTEINS; IMMUNOGLOBULINS; IN VITRO; IN VIVO; LYMPHOCYTES; MEMBRANE PROTEINS; MICE; PATHOGENS; PATIENTS; PEPTIDES; SPECIFICITY; VACCINES

Citation Formats

Yu Hua, Jiang Lifang, Fang Danyun, Yan Huijun, Zhou Jingjiao, Zhou Junmei, Liang Yu, Gao Yang, Zhao, Wei, and Long Beiguo. Selection of SARS-Coronavirus-specific B cell epitopes by phage peptide library screening and evaluation of the immunological effect of epitope-based peptides on mice. United States: N. p., 2007. Web. doi:10.1016/j.virol.2006.09.016.
Yu Hua, Jiang Lifang, Fang Danyun, Yan Huijun, Zhou Jingjiao, Zhou Junmei, Liang Yu, Gao Yang, Zhao, Wei, & Long Beiguo. Selection of SARS-Coronavirus-specific B cell epitopes by phage peptide library screening and evaluation of the immunological effect of epitope-based peptides on mice. United States. doi:10.1016/j.virol.2006.09.016.
Yu Hua, Jiang Lifang, Fang Danyun, Yan Huijun, Zhou Jingjiao, Zhou Junmei, Liang Yu, Gao Yang, Zhao, Wei, and Long Beiguo. Thu . "Selection of SARS-Coronavirus-specific B cell epitopes by phage peptide library screening and evaluation of the immunological effect of epitope-based peptides on mice". United States. doi:10.1016/j.virol.2006.09.016.
@article{osti_20976998,
title = {Selection of SARS-Coronavirus-specific B cell epitopes by phage peptide library screening and evaluation of the immunological effect of epitope-based peptides on mice},
author = {Yu Hua and Jiang Lifang and Fang Danyun and Yan Huijun and Zhou Jingjiao and Zhou Junmei and Liang Yu and Gao Yang and Zhao, Wei and Long Beiguo},
abstractNote = {Antibodies to SARS-Coronavirus (SARS-CoV)-specific B cell epitopes might recognize the pathogen and interrupt its adherence to and penetration of host cells. Hence, these epitopes could be useful for diagnosis and as vaccine constituents. Using the phage-displayed peptide library screening method and purified Fab fragments of immunoglobulin G (IgG Fab) from normal human sera and convalescent sera from SARS-CoV-infected patients as targets, 11 B cell epitopes of SARS-CoV spike glycoprotein (S protein) and membrane protein (M protein) were screened. After a bioinformatics tool was used to analyze these epitopes, four epitope-based S protein dodecapeptides corresponding to the predominant epitopes were chosen for synthesis. Their antigenic specificities and immunogenicities were studied in vitro and in vivo. Flow cytometry and ELISPOT analysis of lymphocytes as well as a serologic analysis of antibody showed that these peptides could trigger a rapid, highly effective, and relatively safe immune response in BALB/c mice. These findings might aid development of SARS diagnostics and vaccines. Moreover, the role of S and M proteins as important surface antigens is confirmed.},
doi = {10.1016/j.virol.2006.09.016},
journal = {Virology},
number = 2,
volume = 359,
place = {United States},
year = {Thu Mar 15 00:00:00 EDT 2007},
month = {Thu Mar 15 00:00:00 EDT 2007}
}