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Title: Endocrine disruption mechanism of o,p'-DDT in mature male tilapia (Oreochromis niloticus)

Abstract

The aim of the present study was to evaluate, in vivo, the potential of o,p'-DDT to disrupt the endocrine system of mature male tilapia. In particular, the possibility that o,p'-DDT effects were mediated directly via the estrogen receptor (ER). Compounds with known ability to bind to the ER were employed: estradiol to induce and tamoxifen to inhibit the estrogenic effects result of the activation of the ER. In addition, an aromatase inhibitor, 4-hydrxyandrostenedione (4-OHA), was used to assess the ability of o,p'-DDT to induce estrogenic effects in a surrounding of low estradiol concentration. The effects of estradiol and o,p'-DDT were studied alone or in the presence of tamoxifen or 4-OHA at the end of a 12-day period of exposure. The main endpoints measured were plasma alkaline-labile phosphorous (ALP; an indirect indicator of vitellogenin), estradiol, testosterone and o,p'-DDT. It was found that o,p'-DDT was able to induce the vitellogenesis (measured as plasma ALP increase) and decrease the circulating levels of estradiol and testosterone. Interestingly, o,p'-DDT kept this ability in whole fish with low concentrations of estradiol which would exclude endogenous estradiol as indirect mediator of the estrogenic effects induced by o,p'-DDT. In addition, the plasma concentration of o,p'-DDT, instead of thatmore » of estradiol, was closely related to the plasma ALP increase induced by o,p'-DDT. This indicates that o,p'-DDT could have directly activated the vitellogenesis. The antiestrogenic action of tamoxifen to inhibit the vitellogenesis and the decrease on plasma estradiol induced by o,p'-DDT indicates that o,p'-DDT can bind directly to the ER. In conclusion, this in vivo study shows that o,p'-DDT has the potential to disrupt the endocrine system and strongly supports that the estrogenic actions of o,p'-DDT involve binding to the ER.« less

Authors:
 [1];  [2];  [3];  [3]
  1. Department of Integrative Biology, University of Guelph, Ontario, N1G 2W1 (Canada). E-mail: olgalidia09@yahoo.com
  2. Department of Integrative Biology, University of Guelph, Ontario, N1G 2W1 (Canada)
  3. Departamento de Recursos del Mar, CINVESTAV Unidad Merida, Carretera Antigua a Progreso km. 6, Ap. Postal 73 'Cordemex', Merida, Yucatan 97310 (Mexico)
Publication Date:
OSTI Identifier:
20976944
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 221; Journal Issue: 2; Other Information: DOI: 10.1016/j.taap.2007.03.011; PII: S0041-008X(07)00108-1; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL INDICATORS; BLOOD PLASMA; DDT; ERBIUM COMPOUNDS; ESTRADIOL; IN VIVO; RECEPTORS; TAMOXIFEN; TESTOSTERONE

Citation Formats

Leanos-Castaneda, Olga, Kraak, Glen van der, Rodriguez-Canul, Rossanna, and Gold, G. Endocrine disruption mechanism of o,p'-DDT in mature male tilapia (Oreochromis niloticus). United States: N. p., 2007. Web. doi:10.1016/j.taap.2007.03.011.
Leanos-Castaneda, Olga, Kraak, Glen van der, Rodriguez-Canul, Rossanna, & Gold, G. Endocrine disruption mechanism of o,p'-DDT in mature male tilapia (Oreochromis niloticus). United States. doi:10.1016/j.taap.2007.03.011.
Leanos-Castaneda, Olga, Kraak, Glen van der, Rodriguez-Canul, Rossanna, and Gold, G. Fri . "Endocrine disruption mechanism of o,p'-DDT in mature male tilapia (Oreochromis niloticus)". United States. doi:10.1016/j.taap.2007.03.011.
@article{osti_20976944,
title = {Endocrine disruption mechanism of o,p'-DDT in mature male tilapia (Oreochromis niloticus)},
author = {Leanos-Castaneda, Olga and Kraak, Glen van der and Rodriguez-Canul, Rossanna and Gold, G.},
abstractNote = {The aim of the present study was to evaluate, in vivo, the potential of o,p'-DDT to disrupt the endocrine system of mature male tilapia. In particular, the possibility that o,p'-DDT effects were mediated directly via the estrogen receptor (ER). Compounds with known ability to bind to the ER were employed: estradiol to induce and tamoxifen to inhibit the estrogenic effects result of the activation of the ER. In addition, an aromatase inhibitor, 4-hydrxyandrostenedione (4-OHA), was used to assess the ability of o,p'-DDT to induce estrogenic effects in a surrounding of low estradiol concentration. The effects of estradiol and o,p'-DDT were studied alone or in the presence of tamoxifen or 4-OHA at the end of a 12-day period of exposure. The main endpoints measured were plasma alkaline-labile phosphorous (ALP; an indirect indicator of vitellogenin), estradiol, testosterone and o,p'-DDT. It was found that o,p'-DDT was able to induce the vitellogenesis (measured as plasma ALP increase) and decrease the circulating levels of estradiol and testosterone. Interestingly, o,p'-DDT kept this ability in whole fish with low concentrations of estradiol which would exclude endogenous estradiol as indirect mediator of the estrogenic effects induced by o,p'-DDT. In addition, the plasma concentration of o,p'-DDT, instead of that of estradiol, was closely related to the plasma ALP increase induced by o,p'-DDT. This indicates that o,p'-DDT could have directly activated the vitellogenesis. The antiestrogenic action of tamoxifen to inhibit the vitellogenesis and the decrease on plasma estradiol induced by o,p'-DDT indicates that o,p'-DDT can bind directly to the ER. In conclusion, this in vivo study shows that o,p'-DDT has the potential to disrupt the endocrine system and strongly supports that the estrogenic actions of o,p'-DDT involve binding to the ER.},
doi = {10.1016/j.taap.2007.03.011},
journal = {Toxicology and Applied Pharmacology},
number = 2,
volume = 221,
place = {United States},
year = {Fri Jun 01 00:00:00 EDT 2007},
month = {Fri Jun 01 00:00:00 EDT 2007}
}