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Title: Nitric oxide and bcl-2 mediated the apoptosis induced by nickel(II) in human T hybridoma cells

Abstract

Although effects of nickel(II) on the immune system have long been recognized, little is known about the effects of nickel(II) on the induction of apoptosis and related signaling events in T cells. In the present study, we investigated the roles and signaling pathways of nickel(II) in the induction of apoptosis in a human T cell line jurkat. The results showed that the cytotoxic effects of Ni involved significant morphological changes and chromosomal condensation (Hoechst 33258 staining). Analyses of hypodiploid cells and FITC-Annexin V and PI double staining showed significant increase of apoptosis in jurkat cells 6, 12 and 24 h after nickel(II) treatment. Flow cytometry analysis also revealed that the loss of mitochondrial membrane potential (MMP) occurred concomitantly with the onset of NiCl{sub 2}-induced apoptosis. Induction of apoptotic cell death by nickel was mediated by reduction of bcl-2 expression. Furthermore, nickel stimulated the generation of nitric oxide (NO). These results suggest that nickel(II) chloride induces jurkat cells apoptosis via nitric oxide generation, mitochondrial depolarization and bcl-2 suppression.

Authors:
 [1];  [1];  [1];  [2]
  1. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093 (China)
  2. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093 (China). E-mail: jhchen@nju.edu.cn
Publication Date:
OSTI Identifier:
20976937
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 221; Journal Issue: 1; Other Information: DOI: 10.1016/j.taap.2007.01.029; PII: S0041-008X(07)00040-3; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; BORON CHLORIDES; DEPOLARIZATION; INHIBITION; MITOCHONDRIA; MORPHOLOGICAL CHANGES; NICKEL; NICKEL CHLORIDES; NITRIC OXIDE

Citation Formats

Guan Fuqin, Zhang Dongmei, Wang Xinchang, and Chen Junhui. Nitric oxide and bcl-2 mediated the apoptosis induced by nickel(II) in human T hybridoma cells. United States: N. p., 2007. Web. doi:10.1016/j.taap.2007.01.029.
Guan Fuqin, Zhang Dongmei, Wang Xinchang, & Chen Junhui. Nitric oxide and bcl-2 mediated the apoptosis induced by nickel(II) in human T hybridoma cells. United States. doi:10.1016/j.taap.2007.01.029.
Guan Fuqin, Zhang Dongmei, Wang Xinchang, and Chen Junhui. Tue . "Nitric oxide and bcl-2 mediated the apoptosis induced by nickel(II) in human T hybridoma cells". United States. doi:10.1016/j.taap.2007.01.029.
@article{osti_20976937,
title = {Nitric oxide and bcl-2 mediated the apoptosis induced by nickel(II) in human T hybridoma cells},
author = {Guan Fuqin and Zhang Dongmei and Wang Xinchang and Chen Junhui},
abstractNote = {Although effects of nickel(II) on the immune system have long been recognized, little is known about the effects of nickel(II) on the induction of apoptosis and related signaling events in T cells. In the present study, we investigated the roles and signaling pathways of nickel(II) in the induction of apoptosis in a human T cell line jurkat. The results showed that the cytotoxic effects of Ni involved significant morphological changes and chromosomal condensation (Hoechst 33258 staining). Analyses of hypodiploid cells and FITC-Annexin V and PI double staining showed significant increase of apoptosis in jurkat cells 6, 12 and 24 h after nickel(II) treatment. Flow cytometry analysis also revealed that the loss of mitochondrial membrane potential (MMP) occurred concomitantly with the onset of NiCl{sub 2}-induced apoptosis. Induction of apoptotic cell death by nickel was mediated by reduction of bcl-2 expression. Furthermore, nickel stimulated the generation of nitric oxide (NO). These results suggest that nickel(II) chloride induces jurkat cells apoptosis via nitric oxide generation, mitochondrial depolarization and bcl-2 suppression.},
doi = {10.1016/j.taap.2007.01.029},
journal = {Toxicology and Applied Pharmacology},
number = 1,
volume = 221,
place = {United States},
year = {Tue May 15 00:00:00 EDT 2007},
month = {Tue May 15 00:00:00 EDT 2007}
}