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Title: Non-invasive fluorescent imaging of gliosis in transgenic mice for profiling developmental neurotoxicity

Abstract

Gliosis is a universal response of Brain to almost all types of neural insults, including neurotoxicity, neurodegeneration, viral infection, and stroke. A hallmark of gliotic reaction is the up-regulation of the astrocytic biomarker GFAP (glial fibrillary acidic protein), which often precedes the anatomically apparent damages in Brain. In this study, neonatal transgenic mice at postnatal day (PD) 4 expressing GFP (green fluorescent protein) under the control of a widely used 2.2-kb human GFAP promoter in Brain are treated with two model neurotoxicants, 1-methyl-4(2'-methylphenyl)-1,2,3,6-tetrahydropyridine (2'-CH{sub 3}-MPTP), and kainic acid (KA), respectively, to induce gliosis. Here we show that the neurotoxicant-induced acute gliosis can be non-invasively imaged and quantified in Brain of conscious (un-anesthetized) mice in real-time, at 0, 2, 4, 6, and 8 h post-toxicant dosing. Therefore the current methodology could be a useful tool for studying the developmental aspects of neuropathies and neurotoxicity.

Authors:
 [1];  [1];  [2]
  1. Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, the Nanos, 04-01, 138669 (Singapore)
  2. Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, the Nanos, 04-01, 138669 (Singapore). E-mail: lzhuo@ibn.a-star.edu.sg
Publication Date:
OSTI Identifier:
20976936
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 221; Journal Issue: 1; Other Information: DOI: 10.1016/j.taap.2007.01.023; PII: S0041-008X(07)00046-4; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL MARKERS; BRAIN; HUMAN POPULATIONS; PROTEINS; SCREENING; TRANSGENIC MICE

Citation Formats

Ho, Gideon, Zhang Chunyan, and Zhuo Lang. Non-invasive fluorescent imaging of gliosis in transgenic mice for profiling developmental neurotoxicity. United States: N. p., 2007. Web. doi:10.1016/j.taap.2007.01.023.
Ho, Gideon, Zhang Chunyan, & Zhuo Lang. Non-invasive fluorescent imaging of gliosis in transgenic mice for profiling developmental neurotoxicity. United States. doi:10.1016/j.taap.2007.01.023.
Ho, Gideon, Zhang Chunyan, and Zhuo Lang. Tue . "Non-invasive fluorescent imaging of gliosis in transgenic mice for profiling developmental neurotoxicity". United States. doi:10.1016/j.taap.2007.01.023.
@article{osti_20976936,
title = {Non-invasive fluorescent imaging of gliosis in transgenic mice for profiling developmental neurotoxicity},
author = {Ho, Gideon and Zhang Chunyan and Zhuo Lang},
abstractNote = {Gliosis is a universal response of Brain to almost all types of neural insults, including neurotoxicity, neurodegeneration, viral infection, and stroke. A hallmark of gliotic reaction is the up-regulation of the astrocytic biomarker GFAP (glial fibrillary acidic protein), which often precedes the anatomically apparent damages in Brain. In this study, neonatal transgenic mice at postnatal day (PD) 4 expressing GFP (green fluorescent protein) under the control of a widely used 2.2-kb human GFAP promoter in Brain are treated with two model neurotoxicants, 1-methyl-4(2'-methylphenyl)-1,2,3,6-tetrahydropyridine (2'-CH{sub 3}-MPTP), and kainic acid (KA), respectively, to induce gliosis. Here we show that the neurotoxicant-induced acute gliosis can be non-invasively imaged and quantified in Brain of conscious (un-anesthetized) mice in real-time, at 0, 2, 4, 6, and 8 h post-toxicant dosing. Therefore the current methodology could be a useful tool for studying the developmental aspects of neuropathies and neurotoxicity.},
doi = {10.1016/j.taap.2007.01.023},
journal = {Toxicology and Applied Pharmacology},
number = 1,
volume = 221,
place = {United States},
year = {Tue May 15 00:00:00 EDT 2007},
month = {Tue May 15 00:00:00 EDT 2007}
}