skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Is plasma {beta}-glucuronidase a novel human biomarker for monitoring anticholinesterase pesticides exposure? A Malaysian experience

Abstract

A cross-sectional study was conducted to investigate the effects of acute and chronic pesticide exposure on the plasma {beta}-glucuronidase enzyme activity among five patients of acute pesticide poisoning in Tengku Ampuan Rahimah Hospital, Klang, 230 farmers in the MADA area, Kedah and 49 fishermen in Setiu, Terengganu. The duration of pesticide exposure among the patients was unknown, but the plasma samples from patients were collected on day one in the hospital. The duration of pesticide exposure among the farmers was between 1 and 45 years. The {beta}-glucuronidase activity was compared with plasma cholinesterase activity in the same individual. The plasma cholinesterase activity was measured using Cholinesterase (PTC) Reagent set kit (Teco Diagnostics, UK) based on colorimetric method, while the plasma {beta}-glucuronidase activity was measured fluorometrically based on {beta}-glucuronidase assay. The plasma cholinesterase activity was significantly reduced (p < 0.05) among the patients (1386.786 {+-} 791.291 U/L/min) but the inhibition in plasma cholinesterase activity among the farmers (7346.5 {+-} 1860.786 U/L/min) was not significant (p > 0.05). The plasma {beta}-glucuronidase activity among the farmers was significantly elevated (p < 0.05) (0.737 {+-} 0.425 {mu}M/h) but not significant among the patients (p > 0.05). The plasma cholinesterase activity was positively correlated withmore » the plasma {beta}-glucuronidase activity among the farmers (r = 0.205, p < 0.01) but not among the patients (r = 0.79, p > 0.05). Thus, plasma {beta}-glucuronidase enzyme activity can be measured as a biomarker for the chronic exposure of pesticide. However, further studies need to be performed to confirm whether plasma {beta}-glucuronidase can be a sensitive biomarker for anticholinesterase pesticide poisoning.« less

Authors:
 [1];  [2]; ; ;  [3]; ; ; ; ;  [2];  [2];  [3];  [3];  [3]; ;  [3]; ;  [4]; ;  [5] more »;  [6] « less
  1. Melaka Institute of Biotechnology, Lot 7, MITC City, 75450 Ayer Keroh, Melaka (Malaysia)|[Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur (Malaysia). E-mail: salmaan@mib.gov.my
  2. Kulliyah of Medicine, International Islamic University of Malaysia, Jalan Istana, Bandar Indera Mahkota, 25200 Kuantan, Pahang (Malaysia)
  3. Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur (Malaysia)
  4. Tengku Ampuan Rahimah Hospital, Klang, Jalan Langat 41200 Klang, Selangor (Malaysia)
  5. Muda Agriculture Development Authority (MADA), Ampang Jajar, 50990 Alor Setar, Kedah (Malaysia)
  6. Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675 (Japan)
Publication Date:
OSTI Identifier:
20976887
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 219; Journal Issue: 2-3; Other Information: DOI: 10.1016/j.taap.2006.10.014; PII: S0041-008X(06)00374-7; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL MARKERS; BLOOD PLASMA; CHOLINESTERASE; CHRONIC EXPOSURE; ENZYME ACTIVITY; GLUCURONIDASE; HOSPITALS; PATIENTS; PESTICIDES; POISONING; SULFUR 49

Citation Formats

Inayat-Hussain, Salmaan H., Lubis, Syarif Husin, Sakian, Noor Ibrahim Mohamed, Ghazali, Ahmad Rohi, Ali, Noor Suhailah, El Sersi, Magdi, Toong, Lee Mun, Zainal, Awang Mat, Hashim, Suhaimi, Ghazali, Mohd Shariman, Saidin, Mohd Nazri, Rahman, Ab Razak Ab, Rafaai, Mohd Jamil Mohd, Omar, Sollahudin, Rapiai, Rafiah, Othman, Radziah, Chan, Lee Tiong, Johari, Amran, Soon, Wong Hing, Salleh, Abdul Rahim, and Satoh, Tetsuo. Is plasma {beta}-glucuronidase a novel human biomarker for monitoring anticholinesterase pesticides exposure? A Malaysian experience. United States: N. p., 2007. Web. doi:10.1016/j.taap.2006.10.014.
Inayat-Hussain, Salmaan H., Lubis, Syarif Husin, Sakian, Noor Ibrahim Mohamed, Ghazali, Ahmad Rohi, Ali, Noor Suhailah, El Sersi, Magdi, Toong, Lee Mun, Zainal, Awang Mat, Hashim, Suhaimi, Ghazali, Mohd Shariman, Saidin, Mohd Nazri, Rahman, Ab Razak Ab, Rafaai, Mohd Jamil Mohd, Omar, Sollahudin, Rapiai, Rafiah, Othman, Radziah, Chan, Lee Tiong, Johari, Amran, Soon, Wong Hing, Salleh, Abdul Rahim, & Satoh, Tetsuo. Is plasma {beta}-glucuronidase a novel human biomarker for monitoring anticholinesterase pesticides exposure? A Malaysian experience. United States. doi:10.1016/j.taap.2006.10.014.
Inayat-Hussain, Salmaan H., Lubis, Syarif Husin, Sakian, Noor Ibrahim Mohamed, Ghazali, Ahmad Rohi, Ali, Noor Suhailah, El Sersi, Magdi, Toong, Lee Mun, Zainal, Awang Mat, Hashim, Suhaimi, Ghazali, Mohd Shariman, Saidin, Mohd Nazri, Rahman, Ab Razak Ab, Rafaai, Mohd Jamil Mohd, Omar, Sollahudin, Rapiai, Rafiah, Othman, Radziah, Chan, Lee Tiong, Johari, Amran, Soon, Wong Hing, Salleh, Abdul Rahim, and Satoh, Tetsuo. Thu . "Is plasma {beta}-glucuronidase a novel human biomarker for monitoring anticholinesterase pesticides exposure? A Malaysian experience". United States. doi:10.1016/j.taap.2006.10.014.
@article{osti_20976887,
title = {Is plasma {beta}-glucuronidase a novel human biomarker for monitoring anticholinesterase pesticides exposure? A Malaysian experience},
author = {Inayat-Hussain, Salmaan H. and Lubis, Syarif Husin and Sakian, Noor Ibrahim Mohamed and Ghazali, Ahmad Rohi and Ali, Noor Suhailah and El Sersi, Magdi and Toong, Lee Mun and Zainal, Awang Mat and Hashim, Suhaimi and Ghazali, Mohd Shariman and Saidin, Mohd Nazri and Rahman, Ab Razak Ab and Rafaai, Mohd Jamil Mohd and Omar, Sollahudin and Rapiai, Rafiah and Othman, Radziah and Chan, Lee Tiong and Johari, Amran and Soon, Wong Hing and Salleh, Abdul Rahim and Satoh, Tetsuo},
abstractNote = {A cross-sectional study was conducted to investigate the effects of acute and chronic pesticide exposure on the plasma {beta}-glucuronidase enzyme activity among five patients of acute pesticide poisoning in Tengku Ampuan Rahimah Hospital, Klang, 230 farmers in the MADA area, Kedah and 49 fishermen in Setiu, Terengganu. The duration of pesticide exposure among the patients was unknown, but the plasma samples from patients were collected on day one in the hospital. The duration of pesticide exposure among the farmers was between 1 and 45 years. The {beta}-glucuronidase activity was compared with plasma cholinesterase activity in the same individual. The plasma cholinesterase activity was measured using Cholinesterase (PTC) Reagent set kit (Teco Diagnostics, UK) based on colorimetric method, while the plasma {beta}-glucuronidase activity was measured fluorometrically based on {beta}-glucuronidase assay. The plasma cholinesterase activity was significantly reduced (p < 0.05) among the patients (1386.786 {+-} 791.291 U/L/min) but the inhibition in plasma cholinesterase activity among the farmers (7346.5 {+-} 1860.786 U/L/min) was not significant (p > 0.05). The plasma {beta}-glucuronidase activity among the farmers was significantly elevated (p < 0.05) (0.737 {+-} 0.425 {mu}M/h) but not significant among the patients (p > 0.05). The plasma cholinesterase activity was positively correlated with the plasma {beta}-glucuronidase activity among the farmers (r = 0.205, p < 0.01) but not among the patients (r = 0.79, p > 0.05). Thus, plasma {beta}-glucuronidase enzyme activity can be measured as a biomarker for the chronic exposure of pesticide. However, further studies need to be performed to confirm whether plasma {beta}-glucuronidase can be a sensitive biomarker for anticholinesterase pesticide poisoning.},
doi = {10.1016/j.taap.2006.10.014},
journal = {Toxicology and Applied Pharmacology},
number = 2-3,
volume = 219,
place = {United States},
year = {Thu Mar 15 00:00:00 EDT 2007},
month = {Thu Mar 15 00:00:00 EDT 2007}
}
  • A nanoparticle-based electrochemical immunosensor has been developed for the detection of phosphorylated acetylcholinesterase (AChE) adducts, which is a potential exposure biomarker for organophosphate pesticides (OP) and chemical warfare nerve agent exposures. Zirconia nanoparticles (ZrO2 NPs) were used as selective sorbents to capture the phosphorylated AChE adduct, and quantum dots (ZnS@CdS, QDs) were used as tags to label monoclonal anti-AChE antibody to track the immunorecognition events. The sandwich-like immunoreactions were performed among the ZrO2 NPs, which were pre-coated on a screen printed electrode (SPE) by electrodeposition, phosphorylated AChE and QD-anti-AChE. The captured QD tags were determined on the SPE by electrochemicalmore » stripping analysis of its metallic component (cadmium) after an acid-dissolution step. Paraoxon was used as a model OP insecticide to prepare the phosphorylated AChE adduct to demonstrate the proof of principle for this sensor technology. The paraoxon-AChE adduct was characterized by Fourier Transform Infrared Spectrum, and the binding affinity of anti-AChE to the paraoxon-AChE was validated with an enzyme-linked immunosorbent assay. The parameters (e.g., amount of ZrO2 NP, QD-anti-AChE concentration,) that govern the electrochemical response of immunosensors were optimized. The voltammetric response of the immunosensor is highly linear over the range of 10 pM to 4 nM paraoxon-AChE, and the limit of detection is estimated to be 8 pM. This new nanoparticle-based electrochemical immunosensor thus provides a sensitive and quantitative tool for biomonitoring exposure to OP pesticides and nerve agents.« less
  • Purpose: To identify a panel of radiation-responsive plasma proteins that could be used in a point-of-care biologic dosimeter to detect clinically significant levels of ionizing radiation exposure. Methods and Materials: Patients undergoing preparation for hematopoietic cell transplantation using radiation therapy (RT) with either total lymphoid irradiation or fractionated total body irradiation were eligible. Plasma was examined from patients with potentially confounding conditions and from normal individuals. Each plasma sample was analyzed for a panel of 17 proteins before RT was begun and at several time points after RT exposure. Paired and unpaired t tests between the dose and control groupsmore » were performed. Conditional inference trees were constructed based on panels of proteins to compare the non-RT group with the RT group. Results: A total of 151 patients (62 RT, 41 infection, 48 trauma) were enrolled on the study, and the plasma from an additional 24 healthy control individuals was analyzed. In comparison with to control individuals, tenascin-C was upregulated and clusterin was downregulated in patients receiving RT. Salivary amylase was strongly radiation responsive, with upregulation in total body irradiation patients and slight downregulation in total lymphoid irradiation patients compared with control individuals. A panel consisting of these 3 proteins accurately distinguished between irradiated patients and healthy control individuals within 3 days after exposure: 97% accuracy, 0.5% false negative rate, 2% false positive rate. The accuracy was diminished when patients with trauma, infection, or both were included (accuracy, 74%-84%; false positive rate, 14%-33%, false negative rate: 8%-40%). Conclusions: A panel of 3 proteins accurately distinguishes unirradiated healthy donors from those exposed to RT (0.8-9.6 Gy) within 3 days of exposure. These findings have significant implications in terms of triaging individuals in the case of nuclear or other radiologic events.« less
  • Acetylcholinesterase (AChE) enzyme activity in red blood cells (RBCs) is a useful biomarker for biomonitoring of exposures to organophosphorus (OP) pesticides and chemical nerve agents. In this paper, we reported a new method for AChE activity assay based on selective immuno-capture of AChE from biological samples followed by enzyme activity assay of captured AChE using a disposable electrochemical sensor. The electrochemical sensor is based on multiwalled carbon nanotubes-gold nanocomposites (MWCNTs-Au) modified screen printed carbon electrode (SPCE). Upon the completion of immunoreaction, the target AChE (including active and inhibited) is captured onto the electrode surface and followed by an electrochemical detectionmore » of enzymatic activity in the presence of acetylthiocholine. A linear response is obtained over standard AChE concentration range from 0.1 to 10 nM. To demonstrate the capability of this new biomonitoring method, AChE solutions dosed with different concentration of paraoxon were used to validate the new AChE assay method. AChE inhibition in OP dosed solutions was proportional to its concentration from 0.2 to 50 nM. The new AChE activity assay method for biomonitoring of OP exposure was further validated with in-vitro paraoxon-dosed RBC samples. The established electrochemical sensing platform for AChE activity assay not only avoids the problem of overlapping substrate specificity with esterases by using selective antibody, but also eliminates potential interference from other electroactive species in biological samples. It offers a new approach for sensitive, selective, and rapid AChE activity assay for biomonitoring of exposures to OPs.« less
  • Novel Fe3O4 at TiO2 magnetic nanoparticles were prepared and developed for a new nanoparticle-based immunosensor for electrochemical quantification of organophosphorylated butyrylcholinesterase (BChE) in plasma, a specific biomarker of exposure to organophosphorus (OP) agents. The Fe3O4 at TiO2 nanoparticles were synthesized by hydrolysis of tetrabutyltitanate on the surface of Fe3O4 magnetic nanospheres, and characterized by attenuated total reflection Fourier-transform infrared spectra, transmission electron microscope and X-ray diffraction. The functional Fe3O4 at TiO2 nanoparticles were performed as capture antibody to selectively enrich phosphorylated moiety instead of phosphoserine antibody in the traditional sandwich immunoassays. The secondary recognition was served by quantum dots (QDs)-taggedmore » anti-BChE antibody (QDs-anti-BChE). With the help of a magnet, the resulting sandwich-like complex, Fe3O4 at TiO2/OP-BChE/QDs-anti-BChE, was easily isolated from sample solutions and the released cadmium ions were detected on a disposable screen-printed electrode (SPE). The binding affinities were investigated by both surface plasmon resonance (SPR) and square wave voltammetry (SWV). This method not only avoids the drawback of unavailability of commercial OP-specific antibody but also amplifies detection signal by QDs-tags together with easy separation of samples by magnetic forces. The proposed immunosensor yields a linear response over a broad OP-BChE concentrations range from 0.02 to 10 nM, with detection limit of 0.01 nM. Moreover, the disposable nanoparticle-based immunosensor has been validated with human plasma samples. It offers a new method for rapid, sensitive, selective and inexpensive screening/evaluating exposure to OP pesticides.« less