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Title: Is plasma {beta}-glucuronidase a novel human biomarker for monitoring anticholinesterase pesticides exposure? A Malaysian experience

Abstract

A cross-sectional study was conducted to investigate the effects of acute and chronic pesticide exposure on the plasma {beta}-glucuronidase enzyme activity among five patients of acute pesticide poisoning in Tengku Ampuan Rahimah Hospital, Klang, 230 farmers in the MADA area, Kedah and 49 fishermen in Setiu, Terengganu. The duration of pesticide exposure among the patients was unknown, but the plasma samples from patients were collected on day one in the hospital. The duration of pesticide exposure among the farmers was between 1 and 45 years. The {beta}-glucuronidase activity was compared with plasma cholinesterase activity in the same individual. The plasma cholinesterase activity was measured using Cholinesterase (PTC) Reagent set kit (Teco Diagnostics, UK) based on colorimetric method, while the plasma {beta}-glucuronidase activity was measured fluorometrically based on {beta}-glucuronidase assay. The plasma cholinesterase activity was significantly reduced (p < 0.05) among the patients (1386.786 {+-} 791.291 U/L/min) but the inhibition in plasma cholinesterase activity among the farmers (7346.5 {+-} 1860.786 U/L/min) was not significant (p > 0.05). The plasma {beta}-glucuronidase activity among the farmers was significantly elevated (p < 0.05) (0.737 {+-} 0.425 {mu}M/h) but not significant among the patients (p > 0.05). The plasma cholinesterase activity was positively correlated withmore » the plasma {beta}-glucuronidase activity among the farmers (r = 0.205, p < 0.01) but not among the patients (r = 0.79, p > 0.05). Thus, plasma {beta}-glucuronidase enzyme activity can be measured as a biomarker for the chronic exposure of pesticide. However, further studies need to be performed to confirm whether plasma {beta}-glucuronidase can be a sensitive biomarker for anticholinesterase pesticide poisoning.« less

Authors:
 [1];  [2]; ; ;  [3]; ; ; ; ;  [2];  [2];  [3];  [3];  [3]; ;  [3]; ;  [4]; ;  [5] more »;  [6] « less
  1. Melaka Institute of Biotechnology, Lot 7, MITC City, 75450 Ayer Keroh, Melaka (Malaysia)|[Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur (Malaysia). E-mail: salmaan@mib.gov.my
  2. Kulliyah of Medicine, International Islamic University of Malaysia, Jalan Istana, Bandar Indera Mahkota, 25200 Kuantan, Pahang (Malaysia)
  3. Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur (Malaysia)
  4. Tengku Ampuan Rahimah Hospital, Klang, Jalan Langat 41200 Klang, Selangor (Malaysia)
  5. Muda Agriculture Development Authority (MADA), Ampang Jajar, 50990 Alor Setar, Kedah (Malaysia)
  6. Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675 (Japan)
Publication Date:
OSTI Identifier:
20976887
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 219; Journal Issue: 2-3; Other Information: DOI: 10.1016/j.taap.2006.10.014; PII: S0041-008X(06)00374-7; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL MARKERS; BLOOD PLASMA; CHOLINESTERASE; CHRONIC EXPOSURE; ENZYME ACTIVITY; GLUCURONIDASE; HOSPITALS; PATIENTS; PESTICIDES; POISONING; SULFUR 49

Citation Formats

Inayat-Hussain, Salmaan H., Lubis, Syarif Husin, Sakian, Noor Ibrahim Mohamed, Ghazali, Ahmad Rohi, Ali, Noor Suhailah, El Sersi, Magdi, Toong, Lee Mun, Zainal, Awang Mat, Hashim, Suhaimi, Ghazali, Mohd Shariman, Saidin, Mohd Nazri, Rahman, Ab Razak Ab, Rafaai, Mohd Jamil Mohd, Omar, Sollahudin, Rapiai, Rafiah, Othman, Radziah, Chan, Lee Tiong, Johari, Amran, Soon, Wong Hing, Salleh, Abdul Rahim, and Satoh, Tetsuo. Is plasma {beta}-glucuronidase a novel human biomarker for monitoring anticholinesterase pesticides exposure? A Malaysian experience. United States: N. p., 2007. Web. doi:10.1016/j.taap.2006.10.014.
Inayat-Hussain, Salmaan H., Lubis, Syarif Husin, Sakian, Noor Ibrahim Mohamed, Ghazali, Ahmad Rohi, Ali, Noor Suhailah, El Sersi, Magdi, Toong, Lee Mun, Zainal, Awang Mat, Hashim, Suhaimi, Ghazali, Mohd Shariman, Saidin, Mohd Nazri, Rahman, Ab Razak Ab, Rafaai, Mohd Jamil Mohd, Omar, Sollahudin, Rapiai, Rafiah, Othman, Radziah, Chan, Lee Tiong, Johari, Amran, Soon, Wong Hing, Salleh, Abdul Rahim, & Satoh, Tetsuo. Is plasma {beta}-glucuronidase a novel human biomarker for monitoring anticholinesterase pesticides exposure? A Malaysian experience. United States. doi:10.1016/j.taap.2006.10.014.
Inayat-Hussain, Salmaan H., Lubis, Syarif Husin, Sakian, Noor Ibrahim Mohamed, Ghazali, Ahmad Rohi, Ali, Noor Suhailah, El Sersi, Magdi, Toong, Lee Mun, Zainal, Awang Mat, Hashim, Suhaimi, Ghazali, Mohd Shariman, Saidin, Mohd Nazri, Rahman, Ab Razak Ab, Rafaai, Mohd Jamil Mohd, Omar, Sollahudin, Rapiai, Rafiah, Othman, Radziah, Chan, Lee Tiong, Johari, Amran, Soon, Wong Hing, Salleh, Abdul Rahim, and Satoh, Tetsuo. Thu . "Is plasma {beta}-glucuronidase a novel human biomarker for monitoring anticholinesterase pesticides exposure? A Malaysian experience". United States. doi:10.1016/j.taap.2006.10.014.
@article{osti_20976887,
title = {Is plasma {beta}-glucuronidase a novel human biomarker for monitoring anticholinesterase pesticides exposure? A Malaysian experience},
author = {Inayat-Hussain, Salmaan H. and Lubis, Syarif Husin and Sakian, Noor Ibrahim Mohamed and Ghazali, Ahmad Rohi and Ali, Noor Suhailah and El Sersi, Magdi and Toong, Lee Mun and Zainal, Awang Mat and Hashim, Suhaimi and Ghazali, Mohd Shariman and Saidin, Mohd Nazri and Rahman, Ab Razak Ab and Rafaai, Mohd Jamil Mohd and Omar, Sollahudin and Rapiai, Rafiah and Othman, Radziah and Chan, Lee Tiong and Johari, Amran and Soon, Wong Hing and Salleh, Abdul Rahim and Satoh, Tetsuo},
abstractNote = {A cross-sectional study was conducted to investigate the effects of acute and chronic pesticide exposure on the plasma {beta}-glucuronidase enzyme activity among five patients of acute pesticide poisoning in Tengku Ampuan Rahimah Hospital, Klang, 230 farmers in the MADA area, Kedah and 49 fishermen in Setiu, Terengganu. The duration of pesticide exposure among the patients was unknown, but the plasma samples from patients were collected on day one in the hospital. The duration of pesticide exposure among the farmers was between 1 and 45 years. The {beta}-glucuronidase activity was compared with plasma cholinesterase activity in the same individual. The plasma cholinesterase activity was measured using Cholinesterase (PTC) Reagent set kit (Teco Diagnostics, UK) based on colorimetric method, while the plasma {beta}-glucuronidase activity was measured fluorometrically based on {beta}-glucuronidase assay. The plasma cholinesterase activity was significantly reduced (p < 0.05) among the patients (1386.786 {+-} 791.291 U/L/min) but the inhibition in plasma cholinesterase activity among the farmers (7346.5 {+-} 1860.786 U/L/min) was not significant (p > 0.05). The plasma {beta}-glucuronidase activity among the farmers was significantly elevated (p < 0.05) (0.737 {+-} 0.425 {mu}M/h) but not significant among the patients (p > 0.05). The plasma cholinesterase activity was positively correlated with the plasma {beta}-glucuronidase activity among the farmers (r = 0.205, p < 0.01) but not among the patients (r = 0.79, p > 0.05). Thus, plasma {beta}-glucuronidase enzyme activity can be measured as a biomarker for the chronic exposure of pesticide. However, further studies need to be performed to confirm whether plasma {beta}-glucuronidase can be a sensitive biomarker for anticholinesterase pesticide poisoning.},
doi = {10.1016/j.taap.2006.10.014},
journal = {Toxicology and Applied Pharmacology},
number = 2-3,
volume = 219,
place = {United States},
year = {Thu Mar 15 00:00:00 EDT 2007},
month = {Thu Mar 15 00:00:00 EDT 2007}
}