Antisense targeting of TGF-{beta}1 augments BMP-induced upregulation of osteopontin, type I collagen and Cbfa1 in human Saos-2 cells
- Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu 702-701 (Korea, Republic of) and Department of Pathology and Laboratory Medicine, Waisman Center for Developmental Disabilities, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53705 (United States)
- Institute of Genetic Engineering, Kyungpook National University, Taegu 702-701 (Korea, Republic of)
- Department of Microbiology, College of Natural Sciences, Kyungpook National University, Taegu 702-701 (Korea, Republic of)
- Department of pathology and Laboratory Medicine, Waisman Center for Developmental Disabilities, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, 53705 (United States)
- Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu 702-701 (Korea, Republic of)
Despite commonalities in signal transduction in osteoblasts from different species, the role of TGF-{beta}1 on bone formation remains elusive. In particular, the role of autocrine TGF-{beta}1 on human osteoblasts is largely unknown. Here we show the effect of TGF-{beta}1 knock-down on the proliferation and differentiation of osteoblasts induced by BMP2. Treatment with antisense TGF-{beta}1 moderately increased the rate of cell proliferation, which was completely reversed by the exogenous addition of TGF-{beta}1. Notably, TGF-{beta}1 blockade significantly enhanced BMP2-induced upregulation of mRNAs encoding osteopontin, type I collagen and Cbfa1, which was suppressed by exogenous TGF-{beta}1. Moreover, TGF-{beta}1 knock-down increased BMP2-induced phosphorylation of Smad1/5 as well as their nuclear import, which paralleled a reduction of inhibitory Smad6. These data suggest autocrine TGF-{beta}1 antagonizes BMP signaling through modulation of inducible Smad6 and the activity of BMP specific Smad1/5.
- OSTI ID:
- 20972140
- Journal Information:
- Experimental Cell Research, Vol. 313, Issue 7; Other Information: DOI: 10.1016/j.yexcr.2007.01.014; PII: S0014-4827(07)00037-7; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
Similar Records
Ski represses BMP signaling in Xenopus and mammalian cells
Proteasome inhibitor MG-132 lowers gastric adenocarcinoma TMK1 cell proliferation via bone morphogenetic protein signaling