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Title: Identification of a population of cells with hematopoietic stem cell properties in mouse aorta-gonad-mesonephros cultures

Abstract

The aorta-gonad-mesonephros (AGM) region is a primary source of definitive hematopoietic cells in the midgestation mouse embryo. In cultures of dispersed AGM regions, adherent cells containing endothelial cells are observed first, and then non-adherent hematopoietic cells are produced. Here we report on the characterization of hematopoietic cells that emerge in the AGM culture. Based on the expression profiles of CD45 and c-Kit, we defined three cell populations: CD45{sup low} c-Kit{sup +} cells that had the ability to form hematopoietic cell colonies in methylcellulose media and in co-cultures with stromal cells; CD45{sup low} c-Kit{sup -} cells that showed a granulocyte morphology; CD45{sup high} c-Kit{sup low/-} that exhibited a macrophage morphology. In co-cultures of OP9 stromal cells and freshly prepared AGM cultures, CD45{sup low} c-Kit{sup +} cells from the AGM culture had the abilities to reproduce CD45{sup low} c-Kit{sup +} cells and differentiate into CD45{sup low} c-Kit{sup -} and CD45{sup high} c-Kit{sup low/-} cells, whereas CD45{sup low} c-Kit{sup -} and CD45{sup high} c-Kit{sup low/-} did not produce CD45{sup low} c-Kit{sup +} cells. Furthermore, CD45{sup low} c-Kit{sup +} cells displayed a long-term repopulating activity in adult hematopoietic tissue when transplanted into the liver of irradiated newborn mice. These results indicate that CD45{sup low}more » c-Kit{sup +} cells from the AGM culture have the potential to reconstitute multi-lineage hematopoietic cells.« less

Authors:
 [1];  [1];  [2];  [3];  [4]
  1. Division of Cell Fate Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto (Japan)
  2. Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, 2-2-1 Honjo, Kumamoto (Japan)
  3. Center for Animal Resources and Development, Kumamoto University, 2-2-1 Honjo, Kumamoto (Japan)
  4. Division of Cell Fate Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto (Japan). E-mail: taga@kaiju.medic.kumamoto-u.ac.jp
Publication Date:
OSTI Identifier:
20972129
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 313; Journal Issue: 5; Other Information: DOI: 10.1016/j.yexcr.2006.12.024; PII: S0014-4827(06)00517-9; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AORTA; BLOOD FORMATION; EMBRYOS; GONADS; INFANTS; IRRADIATION; LEUKOCYTES; LIVER; MACROPHAGES; MICE; MORPHOLOGY; STEM CELLS

Citation Formats

Nobuhisa, Ikuo, Ohtsu, Naoki, Okada, Seiji, Nakagata, Naomi, and Taga, Tetsuya. Identification of a population of cells with hematopoietic stem cell properties in mouse aorta-gonad-mesonephros cultures. United States: N. p., 2007. Web. doi:10.1016/j.yexcr.2006.12.024.
Nobuhisa, Ikuo, Ohtsu, Naoki, Okada, Seiji, Nakagata, Naomi, & Taga, Tetsuya. Identification of a population of cells with hematopoietic stem cell properties in mouse aorta-gonad-mesonephros cultures. United States. doi:10.1016/j.yexcr.2006.12.024.
Nobuhisa, Ikuo, Ohtsu, Naoki, Okada, Seiji, Nakagata, Naomi, and Taga, Tetsuya. Sat . "Identification of a population of cells with hematopoietic stem cell properties in mouse aorta-gonad-mesonephros cultures". United States. doi:10.1016/j.yexcr.2006.12.024.
@article{osti_20972129,
title = {Identification of a population of cells with hematopoietic stem cell properties in mouse aorta-gonad-mesonephros cultures},
author = {Nobuhisa, Ikuo and Ohtsu, Naoki and Okada, Seiji and Nakagata, Naomi and Taga, Tetsuya},
abstractNote = {The aorta-gonad-mesonephros (AGM) region is a primary source of definitive hematopoietic cells in the midgestation mouse embryo. In cultures of dispersed AGM regions, adherent cells containing endothelial cells are observed first, and then non-adherent hematopoietic cells are produced. Here we report on the characterization of hematopoietic cells that emerge in the AGM culture. Based on the expression profiles of CD45 and c-Kit, we defined three cell populations: CD45{sup low} c-Kit{sup +} cells that had the ability to form hematopoietic cell colonies in methylcellulose media and in co-cultures with stromal cells; CD45{sup low} c-Kit{sup -} cells that showed a granulocyte morphology; CD45{sup high} c-Kit{sup low/-} that exhibited a macrophage morphology. In co-cultures of OP9 stromal cells and freshly prepared AGM cultures, CD45{sup low} c-Kit{sup +} cells from the AGM culture had the abilities to reproduce CD45{sup low} c-Kit{sup +} cells and differentiate into CD45{sup low} c-Kit{sup -} and CD45{sup high} c-Kit{sup low/-} cells, whereas CD45{sup low} c-Kit{sup -} and CD45{sup high} c-Kit{sup low/-} did not produce CD45{sup low} c-Kit{sup +} cells. Furthermore, CD45{sup low} c-Kit{sup +} cells displayed a long-term repopulating activity in adult hematopoietic tissue when transplanted into the liver of irradiated newborn mice. These results indicate that CD45{sup low} c-Kit{sup +} cells from the AGM culture have the potential to reconstitute multi-lineage hematopoietic cells.},
doi = {10.1016/j.yexcr.2006.12.024},
journal = {Experimental Cell Research},
number = 5,
volume = 313,
place = {United States},
year = {Sat Mar 10 00:00:00 EST 2007},
month = {Sat Mar 10 00:00:00 EST 2007}
}