skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Eukaryotic translation initiation factor 5A induces apoptosis in colon cancer cells and associates with the nucleus in response to tumour necrosis factor {alpha} signalling

Abstract

Eukaryotic translation initiation factor 5A (eIF5A) is thought to function as a nucleocytoplasmic shuttle protein. There are reports of its involvement in cell proliferation, and more recently it has also been implicated in the regulation of apoptosis. In the present study, we examined the effects of eIF5A over-expression on apoptosis and of siRNA-mediated suppression of eIF5A on expression of the tumour suppressor protein, p53. Over-expression of either eIF5A or a mutant of eIF5A incapable of being hypusinated was found to induce apoptosis in colon carcinoma cells. Our results also indicate that eIF5A is required for expression of p53 following the induction of apoptosis by treatment with Actinomycin D. Depiction of eIF5A localization by indirect immunofluorescence has indicated, for the first time, that the protein is rapidly translocated from the cytoplasm to the nucleus by death receptor activation or following treatment with Actinomycin D. These findings collectively indicate that unhypusinated eIF5A may have pro-apoptotic functions and that eIF5A is rapidly translocated to the nucleus following the induction of apoptotic cell death.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2]
  1. Department of Biology, University of Waterloo, 200 University Ave. W., Waterloo, Ontario, N2L 3G1 (Canada)
  2. Department of Biology, University of Waterloo, 200 University Ave. W., Waterloo, Ontario, N2L 3G1 (Canada). E-mail: jet@sciborg.uwaterloo.ca
Publication Date:
OSTI Identifier:
20972109
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 313; Journal Issue: 3; Other Information: DOI: 10.1016/j.yexcr.2006.09.030; PII: S0014-4827(06)00389-2; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACTINOMYCIN; APOPTOSIS; CARCINOMAS; CELL PROLIFERATION; CYTOPLASM; HYDROXYLASES; INTERFERON; LARGE INTESTINE; NECROSIS; RECEPTORS; RNA

Citation Formats

Taylor, Catherine A., Sun Zhong, Cliche, Dominic O., Ming, Hong, Eshaque, Bithi, Jin Songmu, Hopkins, Marianne T., Thai, Boun, and Thompson, John E.. Eukaryotic translation initiation factor 5A induces apoptosis in colon cancer cells and associates with the nucleus in response to tumour necrosis factor {alpha} signalling. United States: N. p., 2007. Web. doi:10.1016/j.yexcr.2006.09.030.
Taylor, Catherine A., Sun Zhong, Cliche, Dominic O., Ming, Hong, Eshaque, Bithi, Jin Songmu, Hopkins, Marianne T., Thai, Boun, & Thompson, John E.. Eukaryotic translation initiation factor 5A induces apoptosis in colon cancer cells and associates with the nucleus in response to tumour necrosis factor {alpha} signalling. United States. doi:10.1016/j.yexcr.2006.09.030.
Taylor, Catherine A., Sun Zhong, Cliche, Dominic O., Ming, Hong, Eshaque, Bithi, Jin Songmu, Hopkins, Marianne T., Thai, Boun, and Thompson, John E.. Thu . "Eukaryotic translation initiation factor 5A induces apoptosis in colon cancer cells and associates with the nucleus in response to tumour necrosis factor {alpha} signalling". United States. doi:10.1016/j.yexcr.2006.09.030.
@article{osti_20972109,
title = {Eukaryotic translation initiation factor 5A induces apoptosis in colon cancer cells and associates with the nucleus in response to tumour necrosis factor {alpha} signalling},
author = {Taylor, Catherine A. and Sun Zhong and Cliche, Dominic O. and Ming, Hong and Eshaque, Bithi and Jin Songmu and Hopkins, Marianne T. and Thai, Boun and Thompson, John E.},
abstractNote = {Eukaryotic translation initiation factor 5A (eIF5A) is thought to function as a nucleocytoplasmic shuttle protein. There are reports of its involvement in cell proliferation, and more recently it has also been implicated in the regulation of apoptosis. In the present study, we examined the effects of eIF5A over-expression on apoptosis and of siRNA-mediated suppression of eIF5A on expression of the tumour suppressor protein, p53. Over-expression of either eIF5A or a mutant of eIF5A incapable of being hypusinated was found to induce apoptosis in colon carcinoma cells. Our results also indicate that eIF5A is required for expression of p53 following the induction of apoptosis by treatment with Actinomycin D. Depiction of eIF5A localization by indirect immunofluorescence has indicated, for the first time, that the protein is rapidly translocated from the cytoplasm to the nucleus by death receptor activation or following treatment with Actinomycin D. These findings collectively indicate that unhypusinated eIF5A may have pro-apoptotic functions and that eIF5A is rapidly translocated to the nucleus following the induction of apoptotic cell death.},
doi = {10.1016/j.yexcr.2006.09.030},
journal = {Experimental Cell Research},
number = 3,
volume = 313,
place = {United States},
year = {Thu Feb 01 00:00:00 EST 2007},
month = {Thu Feb 01 00:00:00 EST 2007}
}