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Title: The cell cycle and DNA mismatch repair

Abstract

The DNA mismatch repair (MMR) pathway contributes to the fidelity of DNA synthesis and recombination by correcting mispaired nucleotides and insertion/deletion loops (IDLs). We have investigated whether MMR protein expression, activity, and subcellular location are altered during discrete phases of the cell cycle in mammalian cells. Two distinct methods have been used to demonstrate that although physiological MMR protein expression, mismatch binding, and nick-directed MMR activity within the nucleus are at highest levels during S phase, MMR is active throughout the cell cycle. Despite equal MMR nuclear protein concentrations in S and G{sub 2} phases, mismatch binding and repair activities within G{sub 2} are significantly lower, indicating a post-translational decrease in MMR activity specific to G{sub 2}. We further demonstrate that typical co-localization of MutS{alpha} to late S phase replication foci can be disrupted by 2 {mu}M N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). This concentration of MNNG does not decrease ongoing DNA synthesis nor induce cell cycle arrest until the second cell cycle, with long-term colony survival decreased by only 24%. These results suggest that low level alkylation damage can selectively disrupt MMR proofreading activity during DNA synthesis and potentially increase mutation frequency within surviving cells.

Authors:
 [1];  [1];  [1];  [1]
  1. Department of Biochemistry and Cancer Biology, Medical University of Ohio, 3035 Arlington Ave, Toledo, OH 43614-5804 (United States)
Publication Date:
OSTI Identifier:
20972104
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research
Additional Journal Information:
Journal Volume: 313; Journal Issue: 2; Other Information: DOI: 10.1016/j.yexcr.2006.10.018; PII: S0014-4827(06)00436-8; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ALKYLATION; BIOSYNTHESIS; CELL CYCLE; DAMAGE; DNA; DNA MISMATCH; DNA REPAIR; ELUTRIATION; MUTATION FREQUENCY; NUCLEOTIDES; PROTEINS

Citation Formats

Schroering, Allen G, Edelbrock, Michael A, Richards, Timothy J, and Williams, Kandace J. The cell cycle and DNA mismatch repair. United States: N. p., 2007. Web. doi:10.1016/j.yexcr.2006.10.018.
Schroering, Allen G, Edelbrock, Michael A, Richards, Timothy J, & Williams, Kandace J. The cell cycle and DNA mismatch repair. United States. https://doi.org/10.1016/j.yexcr.2006.10.018
Schroering, Allen G, Edelbrock, Michael A, Richards, Timothy J, and Williams, Kandace J. 2007. "The cell cycle and DNA mismatch repair". United States. https://doi.org/10.1016/j.yexcr.2006.10.018.
@article{osti_20972104,
title = {The cell cycle and DNA mismatch repair},
author = {Schroering, Allen G and Edelbrock, Michael A and Richards, Timothy J and Williams, Kandace J},
abstractNote = {The DNA mismatch repair (MMR) pathway contributes to the fidelity of DNA synthesis and recombination by correcting mispaired nucleotides and insertion/deletion loops (IDLs). We have investigated whether MMR protein expression, activity, and subcellular location are altered during discrete phases of the cell cycle in mammalian cells. Two distinct methods have been used to demonstrate that although physiological MMR protein expression, mismatch binding, and nick-directed MMR activity within the nucleus are at highest levels during S phase, MMR is active throughout the cell cycle. Despite equal MMR nuclear protein concentrations in S and G{sub 2} phases, mismatch binding and repair activities within G{sub 2} are significantly lower, indicating a post-translational decrease in MMR activity specific to G{sub 2}. We further demonstrate that typical co-localization of MutS{alpha} to late S phase replication foci can be disrupted by 2 {mu}M N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). This concentration of MNNG does not decrease ongoing DNA synthesis nor induce cell cycle arrest until the second cell cycle, with long-term colony survival decreased by only 24%. These results suggest that low level alkylation damage can selectively disrupt MMR proofreading activity during DNA synthesis and potentially increase mutation frequency within surviving cells.},
doi = {10.1016/j.yexcr.2006.10.018},
url = {https://www.osti.gov/biblio/20972104}, journal = {Experimental Cell Research},
issn = {0014-4827},
number = 2,
volume = 313,
place = {United States},
year = {Mon Jan 15 00:00:00 EST 2007},
month = {Mon Jan 15 00:00:00 EST 2007}
}