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Title: 3-mercaptopropyltrimethoxysilane as insulating coating and surface for protein immobilization for piezoelectric microcantilever sensors

Abstract

We have examined coating (PbMg{sub 1/3}Nb{sub 2/3}O{sub 3}){sub 0.63}-(PbTiO{sub 3}){sub 0.37} (PMN-PT)/tin and lead zirconate titanate (PZT)/glass piezoelectric microcantilever sensor (PEMS) with 3-mercaptopropyl-trimethoxysilane (MPS) by a simple solution method to electrically insulate the PEMS for in-water applications. In contrast to earlier methytrimethoxysilane insulation coating, the MPS coating also facilitated receptor immobilization on the sensor surface via bonding of its sulhydryl group to a bifunctional linker, sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate. We showed that a MPS coating of 21 nm in thickness is sufficient to electrically insulate and provide immobilization surface to the PEMS for in-liquid electrical self-excitation and self-sensing. The in-phosphate buffered saline solution resonance spectra were stable with Q values ranging from 41 to 55. The mass detection sensitivities were determined to be 5x10{sup -11} and 8x10{sup -12} g/Hz for the MPS-insulated PZT-glass and PMN-PT/tin PEMSs, respectively.

Authors:
; ;  [1];  [2];  [2]
  1. Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania 19104 (United States)
  2. (United States)
Publication Date:
OSTI Identifier:
20953435
Resource Type:
Journal Article
Resource Relation:
Journal Name: Review of Scientific Instruments; Journal Volume: 78; Journal Issue: 4; Other Information: DOI: 10.1063/1.2727466; (c) 2007 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; CYCLOHEXANE; DETECTION; EQUIPMENT; GLASS; LIQUIDS; PHOSPHATES; PIEZOELECTRICITY; PZT; RECEPTORS; RESONANCE; SENSITIVITY; SENSORS; SPECTRA; SURFACES; THICKNESS; WATER

Citation Formats

Capobianco, Joseph A., Shih, Wan Y., Shih, W.-H., School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadelphia, Pennsylvania 19104, and Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania 19104. 3-mercaptopropyltrimethoxysilane as insulating coating and surface for protein immobilization for piezoelectric microcantilever sensors. United States: N. p., 2007. Web. doi:10.1063/1.2727466.
Capobianco, Joseph A., Shih, Wan Y., Shih, W.-H., School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadelphia, Pennsylvania 19104, & Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania 19104. 3-mercaptopropyltrimethoxysilane as insulating coating and surface for protein immobilization for piezoelectric microcantilever sensors. United States. doi:10.1063/1.2727466.
Capobianco, Joseph A., Shih, Wan Y., Shih, W.-H., School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadelphia, Pennsylvania 19104, and Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania 19104. Sun . "3-mercaptopropyltrimethoxysilane as insulating coating and surface for protein immobilization for piezoelectric microcantilever sensors". United States. doi:10.1063/1.2727466.
@article{osti_20953435,
title = {3-mercaptopropyltrimethoxysilane as insulating coating and surface for protein immobilization for piezoelectric microcantilever sensors},
author = {Capobianco, Joseph A. and Shih, Wan Y. and Shih, W.-H. and School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadelphia, Pennsylvania 19104 and Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania 19104},
abstractNote = {We have examined coating (PbMg{sub 1/3}Nb{sub 2/3}O{sub 3}){sub 0.63}-(PbTiO{sub 3}){sub 0.37} (PMN-PT)/tin and lead zirconate titanate (PZT)/glass piezoelectric microcantilever sensor (PEMS) with 3-mercaptopropyl-trimethoxysilane (MPS) by a simple solution method to electrically insulate the PEMS for in-water applications. In contrast to earlier methytrimethoxysilane insulation coating, the MPS coating also facilitated receptor immobilization on the sensor surface via bonding of its sulhydryl group to a bifunctional linker, sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate. We showed that a MPS coating of 21 nm in thickness is sufficient to electrically insulate and provide immobilization surface to the PEMS for in-liquid electrical self-excitation and self-sensing. The in-phosphate buffered saline solution resonance spectra were stable with Q values ranging from 41 to 55. The mass detection sensitivities were determined to be 5x10{sup -11} and 8x10{sup -12} g/Hz for the MPS-insulated PZT-glass and PMN-PT/tin PEMSs, respectively.},
doi = {10.1063/1.2727466},
journal = {Review of Scientific Instruments},
number = 4,
volume = 78,
place = {United States},
year = {Sun Apr 15 00:00:00 EDT 2007},
month = {Sun Apr 15 00:00:00 EDT 2007}
}