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Title: A protocol for storage and long-distance shipment of Mediterranean fruit fly (Diptera: Tephritidae) eggs. 1. Effect of temperature, embryo age , and storage time on survival and quality

Abstract

The operational use of Mediterranean fruit fly (medfly) Ceratitis capitata (Wiedemann), genetic sexing strains in Sterile Insect Technique applications can be maximized by developing methods for effective shipment of eggs. This would enable a central production facility to maintain the relevant mother stocks and large colonies to supply eggs to satellite centers that would mass produce only males for irradiation and release. In order to achieve this, the survival of medfly embryos of different ages was assessed after storage at 5, 10, 15, 20, and 25 deg. C in water for different periods of time. Survival was affected by all 3 variables, i.e., embryo age, water temperature, and length of storage. Storage of embryos at any temperature for 120 h resulted in almost no survival. Controlling the age of the embryo at the time of the temperature treatment is crucial for the success of this procedure. Embryos collected between 0 to 12 h after oviposition and pre-incubated at 25 deg. C for 12 h provide a suitable 72 h window for shipment when maintained between 10 to 15 deg. C. Under these conditions, no significant reductions in survival during all the developmental stages were observed. (author) [Spanish] El uso operacionalmore » de cepas de la mosca del mediterraneo Ceratitis capitata (Wiedemann) en las cuales es posible separar los sexos a traves de mecanismos geneticos para su utilizacion en la Tecnica del Insecto Esteril (TIE), puede ser maximizado con el desarrollo de metodos efectivos para el envio y transporte de huevos. Esto permite que un laboratorio de produccion centralizada mantenga las respectivas colonias responsables por la produccion de huevos para este abastecer laboratorios satelites responsables por la produccion masiva de solamente machos para subsiguiente irradiacion y liberacion. Para ser posible esta alternativa fue evaluada la supervivencia de embriones de diferentes edades despues de su almacenamiento en agua a 5, 10, 15, 20 y 25 deg. C por diferentes periodos de tiempo. La supervivencia fue afectada por las 3 variables evaluadas, la edad del embrion, la temperatura del agua y el periodo de almacenamiento. El almacenamiento de los embriones a cualquier temperatura por 120 horas dio como resultado la casi no supervivencia. Una edad controlada de los embriones a tratar es crucial para el exito de este protocolo. Embriones colectados entre 0 a 12 horas despues de la oviposicion y su previa incubacion a 25 deg. C por 12 horas brinda un margen de hasta de 72 horas de duracion del almacenamiento y transporte, siempre y cuando estos se mantengan en una temperatura de entre 10 a 15{sup o}C. En estas condiciones, fue registrada una reduccion no significante de la supervivencia de los diferentes estados de desarrollo. (author)« less

Authors:
; ; ;  [1];  [2]
  1. Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Agency's Laboratories, A-2444 Seibersdorf (Austria)
  2. Programa Moscamed-Guatemala, Planta de Produccion de Mosca del Mediterraneo km 48, Carretera a El Salvador, El Cerinal, Barberena, Santa Rosa (Guatemala)
Publication Date:
OSTI Identifier:
20942916
Resource Type:
Journal Article
Resource Relation:
Journal Name: Florida Entomologist; Journal Volume: 90; Journal Issue: 1; Other Information: Country of input: International Atomic Energy Agency (IAEA); DOI: 10.1653/0015-4040(2007)90[103:APFSAL]2.0.CO;2; Proceedings of a 5-year FAO/IAEA Coordinated Research Project on Fruit Fly Quality, Control, and Behavior; 20 refs, 3 figs, 1 tab
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CERATITIS CAPITATA; EGGS; EMBRYOS; GENETIC VARIABILITY; IRRADIATION; PEST CONTROL; STERILE MALE TECHNIQUE; STORAGE

Citation Formats

Caceres, C., Wornoayporn, V., Islam, S.M., Ahmad, S., and Ramirez, E.. A protocol for storage and long-distance shipment of Mediterranean fruit fly (Diptera: Tephritidae) eggs. 1. Effect of temperature, embryo age , and storage time on survival and quality. United States: N. p., 2007. Web. doi:10.1653/0015-4040(2007)90[103:APFSAL]2.0.CO;2; PROCEEDINGS OF A 5-YEAR FAO/IAEA COORDINATED RESEARCH PROJECT ON FRUIT FLY QUALITY, CONTROL, AND BEHAVIOR; 20 REFS, 3 FIGS, 1 TAB.
Caceres, C., Wornoayporn, V., Islam, S.M., Ahmad, S., & Ramirez, E.. A protocol for storage and long-distance shipment of Mediterranean fruit fly (Diptera: Tephritidae) eggs. 1. Effect of temperature, embryo age , and storage time on survival and quality. United States. doi:10.1653/0015-4040(2007)90[103:APFSAL]2.0.CO;2; PROCEEDINGS OF A 5-YEAR FAO/IAEA COORDINATED RESEARCH PROJECT ON FRUIT FLY QUALITY, CONTROL, AND BEHAVIOR; 20 REFS, 3 FIGS, 1 TAB.
Caceres, C., Wornoayporn, V., Islam, S.M., Ahmad, S., and Ramirez, E.. Thu . "A protocol for storage and long-distance shipment of Mediterranean fruit fly (Diptera: Tephritidae) eggs. 1. Effect of temperature, embryo age , and storage time on survival and quality". United States. doi:10.1653/0015-4040(2007)90[103:APFSAL]2.0.CO;2; PROCEEDINGS OF A 5-YEAR FAO/IAEA COORDINATED RESEARCH PROJECT ON FRUIT FLY QUALITY, CONTROL, AND BEHAVIOR; 20 REFS, 3 FIGS, 1 TAB.
@article{osti_20942916,
title = {A protocol for storage and long-distance shipment of Mediterranean fruit fly (Diptera: Tephritidae) eggs. 1. Effect of temperature, embryo age , and storage time on survival and quality},
author = {Caceres, C. and Wornoayporn, V. and Islam, S.M. and Ahmad, S. and Ramirez, E.},
abstractNote = {The operational use of Mediterranean fruit fly (medfly) Ceratitis capitata (Wiedemann), genetic sexing strains in Sterile Insect Technique applications can be maximized by developing methods for effective shipment of eggs. This would enable a central production facility to maintain the relevant mother stocks and large colonies to supply eggs to satellite centers that would mass produce only males for irradiation and release. In order to achieve this, the survival of medfly embryos of different ages was assessed after storage at 5, 10, 15, 20, and 25 deg. C in water for different periods of time. Survival was affected by all 3 variables, i.e., embryo age, water temperature, and length of storage. Storage of embryos at any temperature for 120 h resulted in almost no survival. Controlling the age of the embryo at the time of the temperature treatment is crucial for the success of this procedure. Embryos collected between 0 to 12 h after oviposition and pre-incubated at 25 deg. C for 12 h provide a suitable 72 h window for shipment when maintained between 10 to 15 deg. C. Under these conditions, no significant reductions in survival during all the developmental stages were observed. (author) [Spanish] El uso operacional de cepas de la mosca del mediterraneo Ceratitis capitata (Wiedemann) en las cuales es posible separar los sexos a traves de mecanismos geneticos para su utilizacion en la Tecnica del Insecto Esteril (TIE), puede ser maximizado con el desarrollo de metodos efectivos para el envio y transporte de huevos. Esto permite que un laboratorio de produccion centralizada mantenga las respectivas colonias responsables por la produccion de huevos para este abastecer laboratorios satelites responsables por la produccion masiva de solamente machos para subsiguiente irradiacion y liberacion. Para ser posible esta alternativa fue evaluada la supervivencia de embriones de diferentes edades despues de su almacenamiento en agua a 5, 10, 15, 20 y 25 deg. C por diferentes periodos de tiempo. La supervivencia fue afectada por las 3 variables evaluadas, la edad del embrion, la temperatura del agua y el periodo de almacenamiento. El almacenamiento de los embriones a cualquier temperatura por 120 horas dio como resultado la casi no supervivencia. Una edad controlada de los embriones a tratar es crucial para el exito de este protocolo. Embriones colectados entre 0 a 12 horas despues de la oviposicion y su previa incubacion a 25 deg. C por 12 horas brinda un margen de hasta de 72 horas de duracion del almacenamiento y transporte, siempre y cuando estos se mantengan en una temperatura de entre 10 a 15{sup o}C. En estas condiciones, fue registrada una reduccion no significante de la supervivencia de los diferentes estados de desarrollo. (author)},
doi = {10.1653/0015-4040(2007)90[103:APFSAL]2.0.CO;2; PROCEEDINGS OF A 5-YEAR FAO/IAEA COORDINATED RESEARCH PROJECT ON FRUIT FLY QUALITY, CONTROL, AND BEHAVIOR; 20 REFS, 3 FIGS, 1 TAB},
journal = {Florida Entomologist},
number = 1,
volume = 90,
place = {United States},
year = {Thu Mar 15 00:00:00 EDT 2007},
month = {Thu Mar 15 00:00:00 EDT 2007}
}