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Title: Effects of pre-irradiation conditioning of Medfly pupae (Diptera: Tephritidae): Hypoxia and quality of sterile males

Abstract

Irradiation of pupae in sterile insect technique (SIT) projects is usually undertaken in hypoxic atmospheres, which have been shown to lessen the deleterious effects of irradiation on the quality of adult sterile flies. Although this is the accepted technology in most mass-rearing and sterilization facilities, to date no information has been generated on the actual levels of oxygen (O{sub 2}) in pupae-packing containers during irradiation. The present study utilized recently-developed technology to investigate the O{sub 2} level inside bags in which pupae of Mediterranean fruit fly (medfly) Ceratitis capitata (Wiedemann) are packed prior to irradiation, the ability of pupae to create hypoxic environments in these bags, and the effect of O{sub 2} atmospheres on the quality of irradiated males. Pupae, 1 d before adult emergence, were shown to deplete the O{sub 2} level in sealed bags in approximately 1 h. The rate of O{sub 2} consumption was dependent upon pupal age and incubation temperature. Incubation temperature did not significantly affect the quality of pupae or mating capacity of resultant adult males if pupae were irradiated under maximal hypoxic conditions inside packing bags. In contrast, mating competitiveness drastically decreased when pupae were irradiated under ambient O{sub 2} conditions, with the packingmore » bag open. There was no difference in the mating capacity of males when pupae were irradiated in sealed bags under either 10% or 2% O{sub 2} levels, or under maximal hypoxia. Normal doses of fluorescent dye, applied to pupae to mark sterile flies, did not affect the ability of pupae to create hypoxic conditions inside packing bags, nor the quality control parameters of either pupae or adults. Current practices in mass-rearing facilities are discussed in the light of these results. (author) [Spanish] La irradiacion de pupas en proyectos de mosca esteril usualmente se hace bajo condiciones de hipoxia. Esta condicion ha demostrado ser menos detrimente a la calidad de las moscas que la irradiacion en atmosferas con proporcion normal de oxigeno. Aunque esta ha sido por mucho tiempo parte del protocolo de irradiacion en plantas de produccion de mosca esteril, hasta ahora no se ha medido el contenido de oxigeno dentro de los recipientes de empaque de pupa durante la irradiacion. El presente estudio investigo los contenidos de O{sub 2} en los contenedores de pupas de la mosca de las frutas del Mediterraneo (Ceratitis capitata Wiedeman), la habilidad de pupas de crear hipoxia dentro de los contenedores, y los efectos del contenido de O{sub 2} durante la irradiacion del contenedor en la calidad y capacidad de apareamiento de moscas esteriles. Pupas de un dia antes de emerger como adultos crearon atmosferas de maxima hipoxia dentro del empaque en aproximadamente una hora. La proporcion de consumo de O{sub 2} en contenedores sellados es dependiente de la edad de la pupa, y de la temperatura de incubacion. La temperatura de incubacion no afecto significativamente la calidad ni la capacidad de apareamiento de machos derivados de pupas irradiadas bajo condiciones de hipoxia. Sin embargo, la capacidad de apareamiento de machos irradiados como pupas en contenedores abiertos y en condiciones oxigenadas fue drasticamente afectada. En comparacion a los resultados anteriores, atmosferas de 2% y 10% O{sub 2} durante la irradiacion no afectaron la capacidad de apareamiento de moscas esteriles. Polvo fluorescente, aplicado a pupas para marcar las moscas esteriles, no tuvo efectos sobre la capacidad de las pupas de crear hipoxia. Los resultados de este estudio se discuten en base a las practicas actuales de produccion e irradiacion. (author)« less

Authors:
;  [1]; ; ;  [2]
  1. Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Beit-Dagan (Israel)
  2. Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Agency's Laboratories, A-2444 Seibersdorf (Austria)
Publication Date:
OSTI Identifier:
20942913
Resource Type:
Journal Article
Resource Relation:
Journal Name: Florida Entomologist; Journal Volume: 90; Journal Issue: 1; Other Information: Country of input: International Atomic Energy Agency (IAEA); DOI: 10.1653/0015-4040(2007)90[80:EOPCOM]2.0.CO;2; Proceedings of a 5-year FAO/IAEA Coordinated Research Project on Fruit Fly Quality, Control, and Behavior; Spanish abstract translation provided by the authors; 10 refs, 6 figs, 2 tabs
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANOXIA; CERATITIS CAPITATA; INCUBATION; IRRADIATION; MASS REARING; MATING; PEST CONTROL; PUPAE; QUALITY CONTROL; RADIOSTERILIZATION; RESPIRATION; STERILE MALE TECHNIQUE

Citation Formats

Nestel, D., Nemny-Lavy, E., Islam, S.M., Wornoayporn, V., and Caceres, C.. Effects of pre-irradiation conditioning of Medfly pupae (Diptera: Tephritidae): Hypoxia and quality of sterile males. United States: N. p., 2007. Web. doi:10.1653/0015-4040(2007)90[80:EOPCOM]2.0.CO;2; PROCEEDINGS OF A 5-YEAR FAO/IAEA COORDINATED RESEARCH PROJECT ON FRUIT FLY QUALITY, CONTROL, AND BEHAVIOR; SPANISH ABSTRACT TRANSLATION PROVIDED BY THE AUTHORS; 10 REFS, 6 FIGS, 2 TABS.
Nestel, D., Nemny-Lavy, E., Islam, S.M., Wornoayporn, V., & Caceres, C.. Effects of pre-irradiation conditioning of Medfly pupae (Diptera: Tephritidae): Hypoxia and quality of sterile males. United States. doi:10.1653/0015-4040(2007)90[80:EOPCOM]2.0.CO;2; PROCEEDINGS OF A 5-YEAR FAO/IAEA COORDINATED RESEARCH PROJECT ON FRUIT FLY QUALITY, CONTROL, AND BEHAVIOR; SPANISH ABSTRACT TRANSLATION PROVIDED BY THE AUTHORS; 10 REFS, 6 FIGS, 2 TABS.
Nestel, D., Nemny-Lavy, E., Islam, S.M., Wornoayporn, V., and Caceres, C.. Thu . "Effects of pre-irradiation conditioning of Medfly pupae (Diptera: Tephritidae): Hypoxia and quality of sterile males". United States. doi:10.1653/0015-4040(2007)90[80:EOPCOM]2.0.CO;2; PROCEEDINGS OF A 5-YEAR FAO/IAEA COORDINATED RESEARCH PROJECT ON FRUIT FLY QUALITY, CONTROL, AND BEHAVIOR; SPANISH ABSTRACT TRANSLATION PROVIDED BY THE AUTHORS; 10 REFS, 6 FIGS, 2 TABS.
@article{osti_20942913,
title = {Effects of pre-irradiation conditioning of Medfly pupae (Diptera: Tephritidae): Hypoxia and quality of sterile males},
author = {Nestel, D. and Nemny-Lavy, E. and Islam, S.M. and Wornoayporn, V. and Caceres, C.},
abstractNote = {Irradiation of pupae in sterile insect technique (SIT) projects is usually undertaken in hypoxic atmospheres, which have been shown to lessen the deleterious effects of irradiation on the quality of adult sterile flies. Although this is the accepted technology in most mass-rearing and sterilization facilities, to date no information has been generated on the actual levels of oxygen (O{sub 2}) in pupae-packing containers during irradiation. The present study utilized recently-developed technology to investigate the O{sub 2} level inside bags in which pupae of Mediterranean fruit fly (medfly) Ceratitis capitata (Wiedemann) are packed prior to irradiation, the ability of pupae to create hypoxic environments in these bags, and the effect of O{sub 2} atmospheres on the quality of irradiated males. Pupae, 1 d before adult emergence, were shown to deplete the O{sub 2} level in sealed bags in approximately 1 h. The rate of O{sub 2} consumption was dependent upon pupal age and incubation temperature. Incubation temperature did not significantly affect the quality of pupae or mating capacity of resultant adult males if pupae were irradiated under maximal hypoxic conditions inside packing bags. In contrast, mating competitiveness drastically decreased when pupae were irradiated under ambient O{sub 2} conditions, with the packing bag open. There was no difference in the mating capacity of males when pupae were irradiated in sealed bags under either 10% or 2% O{sub 2} levels, or under maximal hypoxia. Normal doses of fluorescent dye, applied to pupae to mark sterile flies, did not affect the ability of pupae to create hypoxic conditions inside packing bags, nor the quality control parameters of either pupae or adults. Current practices in mass-rearing facilities are discussed in the light of these results. (author) [Spanish] La irradiacion de pupas en proyectos de mosca esteril usualmente se hace bajo condiciones de hipoxia. Esta condicion ha demostrado ser menos detrimente a la calidad de las moscas que la irradiacion en atmosferas con proporcion normal de oxigeno. Aunque esta ha sido por mucho tiempo parte del protocolo de irradiacion en plantas de produccion de mosca esteril, hasta ahora no se ha medido el contenido de oxigeno dentro de los recipientes de empaque de pupa durante la irradiacion. El presente estudio investigo los contenidos de O{sub 2} en los contenedores de pupas de la mosca de las frutas del Mediterraneo (Ceratitis capitata Wiedeman), la habilidad de pupas de crear hipoxia dentro de los contenedores, y los efectos del contenido de O{sub 2} durante la irradiacion del contenedor en la calidad y capacidad de apareamiento de moscas esteriles. Pupas de un dia antes de emerger como adultos crearon atmosferas de maxima hipoxia dentro del empaque en aproximadamente una hora. La proporcion de consumo de O{sub 2} en contenedores sellados es dependiente de la edad de la pupa, y de la temperatura de incubacion. La temperatura de incubacion no afecto significativamente la calidad ni la capacidad de apareamiento de machos derivados de pupas irradiadas bajo condiciones de hipoxia. Sin embargo, la capacidad de apareamiento de machos irradiados como pupas en contenedores abiertos y en condiciones oxigenadas fue drasticamente afectada. En comparacion a los resultados anteriores, atmosferas de 2% y 10% O{sub 2} durante la irradiacion no afectaron la capacidad de apareamiento de moscas esteriles. Polvo fluorescente, aplicado a pupas para marcar las moscas esteriles, no tuvo efectos sobre la capacidad de las pupas de crear hipoxia. Los resultados de este estudio se discuten en base a las practicas actuales de produccion e irradiacion. (author)},
doi = {10.1653/0015-4040(2007)90[80:EOPCOM]2.0.CO;2; PROCEEDINGS OF A 5-YEAR FAO/IAEA COORDINATED RESEARCH PROJECT ON FRUIT FLY QUALITY, CONTROL, AND BEHAVIOR; SPANISH ABSTRACT TRANSLATION PROVIDED BY THE AUTHORS; 10 REFS, 6 FIGS, 2 TABS},
journal = {Florida Entomologist},
number = 1,
volume = 90,
place = {United States},
year = {Thu Mar 15 00:00:00 EDT 2007},
month = {Thu Mar 15 00:00:00 EDT 2007}
}