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Title: Compatibility and competitiveness of a laboratory strain of Anastrepha Fraterculus (Diptera: Tephritidae) after irradiation treatment

Abstract

We evaluated under semi-natural field cage conditions sexual compatibility and competitiveness of a laboratory strain (LAB) compared to a wild population (TUC) of Anastrepha fraterculus (Wiedemann). The LAB strain is produced under semi-mass rearing conditions at the Estacion Experimental Agroindustrial Obispo Colombres facility (Tucuman, Argentina). Wild flies were obtained at Horco Molle (Tucuman, Argentina) from infested guava fruits. LAB pupae were irradiated ({sup 60}Co) 48 h before adult emergence. The tested doses were 0 (control), 40, 70, and 100 Gy. Twenty-five males and 25 females each of TUC and LAB were released into cages and mating pairs collected. Only 1 irradiation dose was considered at a time. Females were separated and allowed to lay eggs into artificial fruits to estimate induced sterility from the corresponding hatching rate. Copulation start time did not differ significantly between strains nor among irradiation treatments. Copulation duration showed highly significant differences among irradiation doses, but no differences between strains. The index of sexual isolation (ISI) and the relative sterility index (RSI) indices indicated that LAB and TUC are fully compatible, males from TUC and LAB did not differ in mating competitiveness, and irradiation within the range tested did not affect these indices. Non-irradiated LAB femalesmore » exhibited higher mating propensity than TUC ones. However, a significant reduction in the female relative performance index (FRPI) index was observed with increasing irradiation dose. The analysis of induced sterility indicated that treatment with 40 Gy reduces male fertility from about 80% to 0.75%, and higher doses produce total sterility. In females, the 40 Gy dose reduces fertility to about 2% and higher doses prevent egg laying. (author) [Spanish] Se evaluo bajo condiciones semi-naturales en jaulas de campo la compatibilidad y la competitividad sexual de una linea de laboratorio (LAB) con respecto a una poblacion salvaje (TUC) de Anastrepha fraterculus (Wiedemann). La linea de laboratorio se produce en condiciones de cria semi-masiva en las instalaciones de la Estacion Experimental Agroindustrial Obispo Colombres (Tucuman, Argentina). Las moscas salvajes se obtuvieron de frutas infestadas de guayabos en Horco Molle (Tucuman, Argentina). Las pupas de laboratorio fueron irradiadas ({sup 60}Co) 48 horas antes de la emergencia del adulto. Las dosis utilizadas fueron 0 (control), 40, 70, y 100 Gy. Se liberaron 25 machos y 25 hembras de TUC y LAB dentro de las jaulas y se recolectaron las parejas formadas. Solo se considero 1 dosis de irradiacion por vez. Las hembras apareadas fueron separadas y se les permitio poner huevos en frutas artificiales para estimar la esterilidad inducida a traves del porcentaje de eclosion. La hora de inicio de la copula no difirio significativamente entre poblaciones ni entre los tratamientos de irradiacion. La duracion de la copula mostro grandes diferencias entre dosis de irradiacion pero no entre cepas. Los indices ISI (aislamiento) y el RSI (esterilidad relativa) indican que LAB y TUC son totalmente compatibles, los machos de TUC y LAB no difieren en su competitividad y la irradiacion dentro del rango de dosis utilizadas tampoco afecto este indice. Las hembras LAB no irradiadas muestran una mayor propension para el apareamiento que las hembras de TUC. Sin embargo se observo una reduccion significativa del indice FRPI (actuacion relativa de hembras) a medida que se aumenta la dosis de irradiacion. El analisis de la esterilidad inducida indica que con dosis de 40 Gy la fertilidad disminuye del 80% al 0.75%, y con dosis mayores la esterilidad fue total. Las hembras irradiadas con dosis de 40 Gy tienen una fertilidad de aproximadamente 2% y con dosis mayores no ponen huevos. (author)« less

Authors:
 [1]; ; ;  [2]; ; ;  [3]; ; ;  [4]
  1. Comision Nacional de Energia Atomica, CNEA (Argentina)
  2. Depto. Ecologia, Genetica y Evolucion, Facultad de Ciencias Exactas y Naturales, Universidad Buenos Aires (1428) Buenos Aires (Argentina)
  3. Instituto de Genetica, INTA, Castelar (Argentina)
  4. Estacion Experimental Agro-industrial Obispo Colombres, Tucuman (Argentina)
Publication Date:
OSTI Identifier:
20942906
Resource Type:
Journal Article
Resource Relation:
Journal Name: Florida Entomologist; Journal Volume: 90; Journal Issue: 1; Other Information: Country of input: International Atomic Energy Agency (IAEA); DOI: 10.1653/0015-4040(2007)90[27:CACOAL]2.0.CO;2; Proceedings of a 5-year FAO/IAEA Coordinated Research Project on Fruit Fly Quality, Control, and Behavior; Spanish abstract translation provided by the authors; 29 refs, 3 tabs
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANASTREPHA; COBALT 60; EGGS; FERTILITY; FRUITS; HATCHING; IRRADIATION; MASS REARING; MATING; PEST CONTROL; PUPAE; RADIATION DOSES; RADIOSTERILIZATION; STERILE MALE TECHNIQUE; STERILITY

Citation Formats

Allinghi, A., Calcagno, G., Gomez Cendra, P., Vilardi, J.C., Petit-Marty, N., Segura, D., Cladera, J., Vera, T., Gramajo, C., and Willink, E.. Compatibility and competitiveness of a laboratory strain of Anastrepha Fraterculus (Diptera: Tephritidae) after irradiation treatment. United States: N. p., 2007. Web. doi:10.1653/0015-4040(2007)90[27:CACOAL]2.0.CO;2; PROCEEDINGS OF A 5-YEAR FAO/IAEA COORDINATED RESEARCH PROJECT ON FRUIT FLY QUALITY, CONTROL, AND BEHAVIOR; SPANISH ABSTRACT TRANSLATION PROVIDED BY THE AUTHORS; 29 REFS, 3 TABS.
Allinghi, A., Calcagno, G., Gomez Cendra, P., Vilardi, J.C., Petit-Marty, N., Segura, D., Cladera, J., Vera, T., Gramajo, C., & Willink, E.. Compatibility and competitiveness of a laboratory strain of Anastrepha Fraterculus (Diptera: Tephritidae) after irradiation treatment. United States. doi:10.1653/0015-4040(2007)90[27:CACOAL]2.0.CO;2; PROCEEDINGS OF A 5-YEAR FAO/IAEA COORDINATED RESEARCH PROJECT ON FRUIT FLY QUALITY, CONTROL, AND BEHAVIOR; SPANISH ABSTRACT TRANSLATION PROVIDED BY THE AUTHORS; 29 REFS, 3 TABS.
Allinghi, A., Calcagno, G., Gomez Cendra, P., Vilardi, J.C., Petit-Marty, N., Segura, D., Cladera, J., Vera, T., Gramajo, C., and Willink, E.. Thu . "Compatibility and competitiveness of a laboratory strain of Anastrepha Fraterculus (Diptera: Tephritidae) after irradiation treatment". United States. doi:10.1653/0015-4040(2007)90[27:CACOAL]2.0.CO;2; PROCEEDINGS OF A 5-YEAR FAO/IAEA COORDINATED RESEARCH PROJECT ON FRUIT FLY QUALITY, CONTROL, AND BEHAVIOR; SPANISH ABSTRACT TRANSLATION PROVIDED BY THE AUTHORS; 29 REFS, 3 TABS.
@article{osti_20942906,
title = {Compatibility and competitiveness of a laboratory strain of Anastrepha Fraterculus (Diptera: Tephritidae) after irradiation treatment},
author = {Allinghi, A. and Calcagno, G. and Gomez Cendra, P. and Vilardi, J.C. and Petit-Marty, N. and Segura, D. and Cladera, J. and Vera, T. and Gramajo, C. and Willink, E.},
abstractNote = {We evaluated under semi-natural field cage conditions sexual compatibility and competitiveness of a laboratory strain (LAB) compared to a wild population (TUC) of Anastrepha fraterculus (Wiedemann). The LAB strain is produced under semi-mass rearing conditions at the Estacion Experimental Agroindustrial Obispo Colombres facility (Tucuman, Argentina). Wild flies were obtained at Horco Molle (Tucuman, Argentina) from infested guava fruits. LAB pupae were irradiated ({sup 60}Co) 48 h before adult emergence. The tested doses were 0 (control), 40, 70, and 100 Gy. Twenty-five males and 25 females each of TUC and LAB were released into cages and mating pairs collected. Only 1 irradiation dose was considered at a time. Females were separated and allowed to lay eggs into artificial fruits to estimate induced sterility from the corresponding hatching rate. Copulation start time did not differ significantly between strains nor among irradiation treatments. Copulation duration showed highly significant differences among irradiation doses, but no differences between strains. The index of sexual isolation (ISI) and the relative sterility index (RSI) indices indicated that LAB and TUC are fully compatible, males from TUC and LAB did not differ in mating competitiveness, and irradiation within the range tested did not affect these indices. Non-irradiated LAB females exhibited higher mating propensity than TUC ones. However, a significant reduction in the female relative performance index (FRPI) index was observed with increasing irradiation dose. The analysis of induced sterility indicated that treatment with 40 Gy reduces male fertility from about 80% to 0.75%, and higher doses produce total sterility. In females, the 40 Gy dose reduces fertility to about 2% and higher doses prevent egg laying. (author) [Spanish] Se evaluo bajo condiciones semi-naturales en jaulas de campo la compatibilidad y la competitividad sexual de una linea de laboratorio (LAB) con respecto a una poblacion salvaje (TUC) de Anastrepha fraterculus (Wiedemann). La linea de laboratorio se produce en condiciones de cria semi-masiva en las instalaciones de la Estacion Experimental Agroindustrial Obispo Colombres (Tucuman, Argentina). Las moscas salvajes se obtuvieron de frutas infestadas de guayabos en Horco Molle (Tucuman, Argentina). Las pupas de laboratorio fueron irradiadas ({sup 60}Co) 48 horas antes de la emergencia del adulto. Las dosis utilizadas fueron 0 (control), 40, 70, y 100 Gy. Se liberaron 25 machos y 25 hembras de TUC y LAB dentro de las jaulas y se recolectaron las parejas formadas. Solo se considero 1 dosis de irradiacion por vez. Las hembras apareadas fueron separadas y se les permitio poner huevos en frutas artificiales para estimar la esterilidad inducida a traves del porcentaje de eclosion. La hora de inicio de la copula no difirio significativamente entre poblaciones ni entre los tratamientos de irradiacion. La duracion de la copula mostro grandes diferencias entre dosis de irradiacion pero no entre cepas. Los indices ISI (aislamiento) y el RSI (esterilidad relativa) indican que LAB y TUC son totalmente compatibles, los machos de TUC y LAB no difieren en su competitividad y la irradiacion dentro del rango de dosis utilizadas tampoco afecto este indice. Las hembras LAB no irradiadas muestran una mayor propension para el apareamiento que las hembras de TUC. Sin embargo se observo una reduccion significativa del indice FRPI (actuacion relativa de hembras) a medida que se aumenta la dosis de irradiacion. El analisis de la esterilidad inducida indica que con dosis de 40 Gy la fertilidad disminuye del 80% al 0.75%, y con dosis mayores la esterilidad fue total. Las hembras irradiadas con dosis de 40 Gy tienen una fertilidad de aproximadamente 2% y con dosis mayores no ponen huevos. (author)},
doi = {10.1653/0015-4040(2007)90[27:CACOAL]2.0.CO;2; PROCEEDINGS OF A 5-YEAR FAO/IAEA COORDINATED RESEARCH PROJECT ON FRUIT FLY QUALITY, CONTROL, AND BEHAVIOR; SPANISH ABSTRACT TRANSLATION PROVIDED BY THE AUTHORS; 29 REFS, 3 TABS},
journal = {Florida Entomologist},
number = 1,
volume = 90,
place = {United States},
year = {Thu Mar 15 00:00:00 EDT 2007},
month = {Thu Mar 15 00:00:00 EDT 2007}
}