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Title: Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins

Abstract

Despite extensive studies, the hematopoietic versus hepatic origin of liver progenitor oval cells remains controversial. The aim of this study was to determine the origin of such cells after liver injury and to establish an oval cell line. Rat liver injury was induced by subcutaneous insertion of 2-AAF pellets for 7 days with subsequent injection of CCl{sub 4}. Livers were removed 9 to 13 days post-CCl{sub 4} treatment. Immunohistochemistry was performed using anti-c-kit, OV6, Thy1, CK19, AFP, vWF and Rab3b. Isolated non-parenchymal cells were grown on mouse embryonic fibroblast, and their gene expression profile was characterized by RT-PCR. We identified a subpopulation of OV6/CK19/Rab3b-expressing cells that was activated in the periportal region of traumatized livers. We also characterized a second subpopulation that expressed the HSCs marker c-kit but not Thy1. Although we successfully isolated both cell types, OV6/CK19/Rab3b{sup +} cells fail to propagate while c-kit {sup +}-HSCs appeared to proliferate for up to 7 weeks. Cells formed clusters which expressed c-kit, Thy1 and albumin. Our results indicate that a bona fide oval progenitor cell population resides within the liver and is distinct from c-kit {sup +}-HSCs. Oval cells require the hepatic niche to proliferate, while cells mobilized from the circulationmore » proliferate and transdifferentiate into hepatocytes without evidence of cell fusion.« less

Authors:
 [1];  [2];  [1];  [1];  [1]
  1. Department of Cell Physiology and Metabolism, University Medical Center, 1211 Geneva 4 (Switzerland)
  2. Division of Clinical Pharmacology and Toxicology, Zurich (Switzerland)
Publication Date:
OSTI Identifier:
20858016
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research
Additional Journal Information:
Journal Volume: 312; Journal Issue: 15; Other Information: DOI: 10.1016/j.yexcr.2006.05.007; PII: S0014-4827(06)00189-3; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACETYLAMINOFLUORENES; ALBUMINS; CARBON TETRACHLORIDE; FIBROBLASTS; GENES; INJECTION; INJURIES; LIVER; LIVER CELLS; MICE; POLYMERASE CHAIN REACTION; RATS; STEM CELLS

Citation Formats

Corcelle, V, Stieger, B, Gjinovci, A, Wollheim, C B, and Gauthier, B R. Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins. United States: N. p., 2006. Web. doi:10.1016/j.yexcr.2006.05.007.
Corcelle, V, Stieger, B, Gjinovci, A, Wollheim, C B, & Gauthier, B R. Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins. United States. https://doi.org/10.1016/j.yexcr.2006.05.007
Corcelle, V, Stieger, B, Gjinovci, A, Wollheim, C B, and Gauthier, B R. 2006. "Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins". United States. https://doi.org/10.1016/j.yexcr.2006.05.007.
@article{osti_20858016,
title = {Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins},
author = {Corcelle, V and Stieger, B and Gjinovci, A and Wollheim, C B and Gauthier, B R},
abstractNote = {Despite extensive studies, the hematopoietic versus hepatic origin of liver progenitor oval cells remains controversial. The aim of this study was to determine the origin of such cells after liver injury and to establish an oval cell line. Rat liver injury was induced by subcutaneous insertion of 2-AAF pellets for 7 days with subsequent injection of CCl{sub 4}. Livers were removed 9 to 13 days post-CCl{sub 4} treatment. Immunohistochemistry was performed using anti-c-kit, OV6, Thy1, CK19, AFP, vWF and Rab3b. Isolated non-parenchymal cells were grown on mouse embryonic fibroblast, and their gene expression profile was characterized by RT-PCR. We identified a subpopulation of OV6/CK19/Rab3b-expressing cells that was activated in the periportal region of traumatized livers. We also characterized a second subpopulation that expressed the HSCs marker c-kit but not Thy1. Although we successfully isolated both cell types, OV6/CK19/Rab3b{sup +} cells fail to propagate while c-kit {sup +}-HSCs appeared to proliferate for up to 7 weeks. Cells formed clusters which expressed c-kit, Thy1 and albumin. Our results indicate that a bona fide oval progenitor cell population resides within the liver and is distinct from c-kit {sup +}-HSCs. Oval cells require the hepatic niche to proliferate, while cells mobilized from the circulation proliferate and transdifferentiate into hepatocytes without evidence of cell fusion.},
doi = {10.1016/j.yexcr.2006.05.007},
url = {https://www.osti.gov/biblio/20858016}, journal = {Experimental Cell Research},
issn = {0014-4827},
number = 15,
volume = 312,
place = {United States},
year = {2006},
month = {9}
}