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Title: A putative transcriptional elongation factor hIws1 is essential for mammalian cell proliferation

Abstract

Iws1 has been implicated in transcriptional elongation by interaction with RNA polymerase II (RNAP II) and elongation factor Spt6 in budding yeast Saccharomyces cerevisiae, and association with transcription factor TFIIS in mammalian cells, but its role in controlling cell growth and proliferation remains unknown. Here we report that the human homolog of Iws1, hIws1, physically interacts with protein arginine methyltransferases PRMT5 which methylates elongation factor Spt5 and regulates its interaction with RNA polymerase II. Gene-specific silencing of hIws1 by RNA interference reveals that hIws1 is essential for cell viability. GFP fusion protein expression approaches demonstrate that the hIws1 protein is located in the nucleus, subsequently, two regions harbored within the hIws1 protein are demonstrated to contain nuclear localization signals (NLSs). In addition, mouse homolog of hiws1 is found to express ubiquitously in various tissues.

Authors:
 [1];  [2];  [1];  [3];  [4]
  1. Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing 100101 (China)
  2. (China)
  3. Animal Sci.-Tech. College, Yangzhou University, Yangzhou, Jiangsu 225005 (China)
  4. Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing 100101 (China). E-mail: slbao@genetics.ac.cn
Publication Date:
OSTI Identifier:
20857977
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 353; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2006.11.133; PII: S0006-291X(06)02584-8; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ARGININE; CELL PROLIFERATION; ELONGATION; GENES; MICE; POLYMERASE CHAIN REACTION; RNA; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION FACTORS

Citation Formats

Liu Zhangguo, School of Animal Science, Zhejiang Forestry University, Hangzhou, Zhejiang 311300, Zhou Zhongwei, Chen Guohong, and Bao Shilai. A putative transcriptional elongation factor hIws1 is essential for mammalian cell proliferation. United States: N. p., 2007. Web.
Liu Zhangguo, School of Animal Science, Zhejiang Forestry University, Hangzhou, Zhejiang 311300, Zhou Zhongwei, Chen Guohong, & Bao Shilai. A putative transcriptional elongation factor hIws1 is essential for mammalian cell proliferation. United States.
Liu Zhangguo, School of Animal Science, Zhejiang Forestry University, Hangzhou, Zhejiang 311300, Zhou Zhongwei, Chen Guohong, and Bao Shilai. Fri . "A putative transcriptional elongation factor hIws1 is essential for mammalian cell proliferation". United States. doi:.
@article{osti_20857977,
title = {A putative transcriptional elongation factor hIws1 is essential for mammalian cell proliferation},
author = {Liu Zhangguo and School of Animal Science, Zhejiang Forestry University, Hangzhou, Zhejiang 311300 and Zhou Zhongwei and Chen Guohong and Bao Shilai},
abstractNote = {Iws1 has been implicated in transcriptional elongation by interaction with RNA polymerase II (RNAP II) and elongation factor Spt6 in budding yeast Saccharomyces cerevisiae, and association with transcription factor TFIIS in mammalian cells, but its role in controlling cell growth and proliferation remains unknown. Here we report that the human homolog of Iws1, hIws1, physically interacts with protein arginine methyltransferases PRMT5 which methylates elongation factor Spt5 and regulates its interaction with RNA polymerase II. Gene-specific silencing of hIws1 by RNA interference reveals that hIws1 is essential for cell viability. GFP fusion protein expression approaches demonstrate that the hIws1 protein is located in the nucleus, subsequently, two regions harbored within the hIws1 protein are demonstrated to contain nuclear localization signals (NLSs). In addition, mouse homolog of hiws1 is found to express ubiquitously in various tissues.},
doi = {},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 353,
place = {United States},
year = {Fri Feb 02 00:00:00 EST 2007},
month = {Fri Feb 02 00:00:00 EST 2007}
}