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Title: Proliferating cell nuclear antigen (PCNA) interacts with a meiosis-specific RecA homologues, Lim15/Dmc1, but does not stimulate its strand transfer activity

Abstract

PCNA is a multi-functional protein that is involved in various nuclear events. Here we show that PCNA participates in events occurring during early meiotic prophase. Analysis of protein-protein interactions using surface plasmon resonance indicates that Coprinus cinereus PCNA (CoPCNA) specifically interacts with a meiotic specific RecA-like factor, C. cinereus Lim15/Dmc1 (CoLim15) in vitro. The binding efficiency increases with addition of Mg{sup 2+} ions, while ATP inhibits the interaction. Co-immunoprecipitation experiments indicate that the CoLim15 protein interacts with the CoPCNA protein in vitro and in the cell extracts. Despite the interaction between these two factors, no enhancement of CoLim15-dependent strand transfer activity by CoPCNA was found in vitro. We propose that the interaction between Lim15/Dmc1 and PCNA mediates the recombination-associated DNA synthesis during meiosis.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2];  [3]
  1. Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510 (Japan)
  2. Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510 (Japan). E-mail: kengo@rs.noda.tus.ac.jp
  3. Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N., A3-025, Seattle, WA 98109 (United States)
Publication Date:
OSTI Identifier:
20857970
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 352; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2006.11.094; PII: S0006-291X(06)02511-3; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIGENS; ATP; BIOSYNTHESIS; DNA; DNA REPLICATION; IN VITRO; MEIOSIS; MITOSIS; PROTEINS; RECOMBINATION

Citation Formats

Hamada, Fumika N., Koshiyama, Akiyo, Namekawa, Satoshi H., Ishii, Satomi, Iwabata, Kazuki, Sugawara, Hiroko, Nara, Takayuki Y., Sakaguchi, Kengo, and Sawado, Tomoyuki. Proliferating cell nuclear antigen (PCNA) interacts with a meiosis-specific RecA homologues, Lim15/Dmc1, but does not stimulate its strand transfer activity. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2006.11.094.
Hamada, Fumika N., Koshiyama, Akiyo, Namekawa, Satoshi H., Ishii, Satomi, Iwabata, Kazuki, Sugawara, Hiroko, Nara, Takayuki Y., Sakaguchi, Kengo, & Sawado, Tomoyuki. Proliferating cell nuclear antigen (PCNA) interacts with a meiosis-specific RecA homologues, Lim15/Dmc1, but does not stimulate its strand transfer activity. United States. doi:10.1016/j.bbrc.2006.11.094.
Hamada, Fumika N., Koshiyama, Akiyo, Namekawa, Satoshi H., Ishii, Satomi, Iwabata, Kazuki, Sugawara, Hiroko, Nara, Takayuki Y., Sakaguchi, Kengo, and Sawado, Tomoyuki. Fri . "Proliferating cell nuclear antigen (PCNA) interacts with a meiosis-specific RecA homologues, Lim15/Dmc1, but does not stimulate its strand transfer activity". United States. doi:10.1016/j.bbrc.2006.11.094.
@article{osti_20857970,
title = {Proliferating cell nuclear antigen (PCNA) interacts with a meiosis-specific RecA homologues, Lim15/Dmc1, but does not stimulate its strand transfer activity},
author = {Hamada, Fumika N. and Koshiyama, Akiyo and Namekawa, Satoshi H. and Ishii, Satomi and Iwabata, Kazuki and Sugawara, Hiroko and Nara, Takayuki Y. and Sakaguchi, Kengo and Sawado, Tomoyuki},
abstractNote = {PCNA is a multi-functional protein that is involved in various nuclear events. Here we show that PCNA participates in events occurring during early meiotic prophase. Analysis of protein-protein interactions using surface plasmon resonance indicates that Coprinus cinereus PCNA (CoPCNA) specifically interacts with a meiotic specific RecA-like factor, C. cinereus Lim15/Dmc1 (CoLim15) in vitro. The binding efficiency increases with addition of Mg{sup 2+} ions, while ATP inhibits the interaction. Co-immunoprecipitation experiments indicate that the CoLim15 protein interacts with the CoPCNA protein in vitro and in the cell extracts. Despite the interaction between these two factors, no enhancement of CoLim15-dependent strand transfer activity by CoPCNA was found in vitro. We propose that the interaction between Lim15/Dmc1 and PCNA mediates the recombination-associated DNA synthesis during meiosis.},
doi = {10.1016/j.bbrc.2006.11.094},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 352,
place = {United States},
year = {Fri Jan 26 00:00:00 EST 2007},
month = {Fri Jan 26 00:00:00 EST 2007}
}