Characterization of a novel oncogenic K-ras mutation in colon cancer
- Molecular Diagnosis and Cancer Prevention Division, Saitama Cancer Center, 818 Komuro Ina, Kitaadachigun, Saitama 362-0806 (Japan) and Research Institute for Clinical Oncology, Saitama Cancer Center, 818 Komuro Ina, Kitaadachigun, Saitama 362-0806 (Japan)
- Molecular Diagnosis and Cancer Prevention Division, Saitama Cancer Center, 818 Komuro Ina, Kitaadachigun, Saitama 362-0806 (Japan)
- Gastroenterology Division, Saitama Cancer Center, 818 Komuro Ina, Kitaadachigun, Saitama 362-0806 (Japan)
- Research Institute for Clinical Oncology, Saitama Cancer Center, 818 Komuro Ina, Kitaadachigun, Saitama 362-0806 (Japan)
Activating mutations of RAS are frequently observed in subsets of human cancers, indicating that RAS activation is involved in tumorigenesis. Here, we identified and characterized a novel G to T transversion mutation of the K-ras gene at the third position of codon 19 (TTG) which substituted phenylalanine for leucine in 3 primary colon carcinomas. Biological and biochemical activity was examined using transformed NIH3T3 cells expressing mutant or wild-type K-ras. Transformants harboring the K-ras mutation at codon 19 showed proliferative capacity under serum-starved conditions, less contact inhibition, anchorage-independent growth, tumorigenicity in nude mice and elevation of active Ras-GTP levels. These results indicated that this novel mutation possesses high oncogenic activity.
- OSTI ID:
- 20857968
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 352, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2006.11.091; PII: S0006-291X(06)02560-5; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Novel mutation of the c-K-ras oncogene activated in a human lung carcinoma
Multiple oncogene activation in a radiation carcinogenesis model