Tat-mediated protein delivery in living Caenorhabditis elegans
- Department of Surgery, McGill University, Montreal, Que. (Canada)
- Department of Medicine, McGill University, Montreal, Que. (Canada)
- Department of Surgery, McGill University, Montreal, Que. (Canada) and Department of Medicine, McGill University, Montreal, Que. (Canada) and Department of Anatomy and Cell Biology, McGill University, Montreal, Que. (Canada) and INSERM, E362, Universite Bordeaux 2, Bordeaux (France)
The Tat protein from HIV-1 fused with heterologous proteins traverses biological membranes in a transcellular process called: protein transduction. This has already been successfully exploited in various biological models, but never in the nematode worm Caenorhabditis elegans. TAT-eGFP or GST-eGFP proteins were fed to C. elegans worms, which resulted in the specific localization of Tat-eGFP to epithelial intestinal cells. This system represents an efficient tool for transcellular transduction in C. elegans intestinal cells. Indeed, this approach avoids the use of tedious purification steps to purify the TAT fusion proteins and allows for rapid analyses of the transduced proteins. In addition, it may represent an efficient tool to functionally analyze the mechanisms of protein transduction as well as to complement RNAi/KO in the epithelial intestinal system. To sum up, the advantage of this technology is to combine the potential of bacterial expression system and the Tat-mediated transduction technique in living worm.
- OSTI ID:
- 20857961
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 352, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2006.11.046; PII: S0006-291X(06)02506-X; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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