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Title: Identification of peroxisome-proliferator responsive element in the mouse HSL gene

Abstract

Hormone-sensitive lipase (HSL) catalyzes the rate-limiting step of lipolysis in adipose tissue. Several studies suggest that protein phosphorylation regulates the HSL enzymatic activity. On the other hand, the precise mechanism of the transcriptional regulation of the HSL gene remains to be elucidated. Here, we identified a functional peroxisome-proliferator responsive element (PPRE) in the mouse HSL promoter by reporter assay in CV-1 cells using serial deletion and point mutants of the 5'-flanking region. Chromatin immunoprecipitation (ChIP) analysis revealed that both peroxisome-proliferator activated receptor (PPAR{gamma}) and retinoid X receptor (RXR{alpha}) interacted with the region. Binding of the PPAR{gamma}/RXR{alpha} heterodimer to the PPRE sequence was also confirmed by electrophoretic mobility shift assay. These results indicate that the HSL gene is transcriptionally regulated by PPAR{gamma}/RXR{alpha} heterodimer, and suggest that a cis-acting element regulates the HSL gene expression.

Authors:
 [1];  [2];  [2];  [2]
  1. Central Laboratories for Frontier Technology, Kirin Brewery Co., Ltd., Kanazawa-ku Fukuura 1-13-5, Yokohama 236-0004 (Japan). E-mail: hyajima@kirin.co.jp
  2. Central Laboratories for Frontier Technology, Kirin Brewery Co., Ltd., Kanazawa-ku Fukuura 1-13-5, Yokohama 236-0004 (Japan)
Publication Date:
OSTI Identifier:
20857959
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 352; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2006.11.054; PII: S0006-291X(06)02525-3; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADIPOSE TISSUE; CHROMATIN; GENE REGULATION; GENES; HORMONES; LIPASES; MICE; MUTANTS; PHOSPHORYLATION; PROMOTERS; RECEPTORS

Citation Formats

Yajima, Hiroaki, Kobayashi, Yumie, Kanaya, Tomoka, and Horino, Yoko. Identification of peroxisome-proliferator responsive element in the mouse HSL gene. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2006.11.054.
Yajima, Hiroaki, Kobayashi, Yumie, Kanaya, Tomoka, & Horino, Yoko. Identification of peroxisome-proliferator responsive element in the mouse HSL gene. United States. doi:10.1016/j.bbrc.2006.11.054.
Yajima, Hiroaki, Kobayashi, Yumie, Kanaya, Tomoka, and Horino, Yoko. Fri . "Identification of peroxisome-proliferator responsive element in the mouse HSL gene". United States. doi:10.1016/j.bbrc.2006.11.054.
@article{osti_20857959,
title = {Identification of peroxisome-proliferator responsive element in the mouse HSL gene},
author = {Yajima, Hiroaki and Kobayashi, Yumie and Kanaya, Tomoka and Horino, Yoko},
abstractNote = {Hormone-sensitive lipase (HSL) catalyzes the rate-limiting step of lipolysis in adipose tissue. Several studies suggest that protein phosphorylation regulates the HSL enzymatic activity. On the other hand, the precise mechanism of the transcriptional regulation of the HSL gene remains to be elucidated. Here, we identified a functional peroxisome-proliferator responsive element (PPRE) in the mouse HSL promoter by reporter assay in CV-1 cells using serial deletion and point mutants of the 5'-flanking region. Chromatin immunoprecipitation (ChIP) analysis revealed that both peroxisome-proliferator activated receptor (PPAR{gamma}) and retinoid X receptor (RXR{alpha}) interacted with the region. Binding of the PPAR{gamma}/RXR{alpha} heterodimer to the PPRE sequence was also confirmed by electrophoretic mobility shift assay. These results indicate that the HSL gene is transcriptionally regulated by PPAR{gamma}/RXR{alpha} heterodimer, and suggest that a cis-acting element regulates the HSL gene expression.},
doi = {10.1016/j.bbrc.2006.11.054},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 352,
place = {United States},
year = {Fri Jan 12 00:00:00 EST 2007},
month = {Fri Jan 12 00:00:00 EST 2007}
}