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Title: All-trans retinoic acid negatively regulates cytotoxic activities of nature killer cell line 92

Abstract

NK cells are key components of innate immune systems and their activities are regulated by cytokines and hormones. All-trans retinoic acid (ATRA), as a metabolite of vitamin A and an immunomodulatory hormone, plays an important role in regulating immune responses. In the present study, we investigated the effect of ATRA on human NK cell line NK92. We found that ATRA dose-dependently suppressed cytotoxic activities of NK92 cells without affecting their proliferation. To explore the mechanisms underlying the ATRA influence on NK92 cells, we examined the production of cytokines (TNF-{alpha}, IFN-{gamma}), gene expression of cytotoxic-associated molecules (perforin, granzyme B, nature killer receptors (NCRs), and NKG2D), and the activation of NF-{kappa}B pathways related with immune response. Our results demonstrated that ATRA suppressed NF-{kappa}B activity and prevented I{kappa}B{alpha} degradation in a dose-dependent way, inhibited IFN-{gamma} production and gene expression of granzyme B and NKp46. Our findings suggest that ATRA is a negative regulator of NK92 cell activation and may act as a potential regulator of anti-inflammatory functions in vivo.

Authors:
 [1];  [2];  [2];  [3];  [2];  [2];  [2];  [2]
  1. School of life science, Tongji university, 1239 Siping Road, Shanghai 200092 (China). E-mail: liang3829@sina.com.cn
  2. School of life science, Tongji university, 1239 Siping Road, Shanghai 200092 (China)
  3. Department of Public Health and Molecular Biology, Shanghai Municipal Center for Disease Control and Prevention (China)
Publication Date:
OSTI Identifier:
20857947
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 352; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2006.10.132; PII: S0006-291X(06)02395-3; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; GENES; HORMONES; IN VIVO; INFLAMMATION; LYMPHOKINES; MOLECULES; NATURAL KILLER CELLS; RECEPTORS; RETINOIC ACID; VITAMIN A

Citation Formats

Li Ang, He Meilan, Wang Hui, Qiao Bin, Chen Ping, Gu Hua, Zhang Mengjie, and He Shengxiang. All-trans retinoic acid negatively regulates cytotoxic activities of nature killer cell line 92. United States: N. p., 2007. Web.
Li Ang, He Meilan, Wang Hui, Qiao Bin, Chen Ping, Gu Hua, Zhang Mengjie, & He Shengxiang. All-trans retinoic acid negatively regulates cytotoxic activities of nature killer cell line 92. United States.
Li Ang, He Meilan, Wang Hui, Qiao Bin, Chen Ping, Gu Hua, Zhang Mengjie, and He Shengxiang. Fri . "All-trans retinoic acid negatively regulates cytotoxic activities of nature killer cell line 92". United States. doi:.
@article{osti_20857947,
title = {All-trans retinoic acid negatively regulates cytotoxic activities of nature killer cell line 92},
author = {Li Ang and He Meilan and Wang Hui and Qiao Bin and Chen Ping and Gu Hua and Zhang Mengjie and He Shengxiang},
abstractNote = {NK cells are key components of innate immune systems and their activities are regulated by cytokines and hormones. All-trans retinoic acid (ATRA), as a metabolite of vitamin A and an immunomodulatory hormone, plays an important role in regulating immune responses. In the present study, we investigated the effect of ATRA on human NK cell line NK92. We found that ATRA dose-dependently suppressed cytotoxic activities of NK92 cells without affecting their proliferation. To explore the mechanisms underlying the ATRA influence on NK92 cells, we examined the production of cytokines (TNF-{alpha}, IFN-{gamma}), gene expression of cytotoxic-associated molecules (perforin, granzyme B, nature killer receptors (NCRs), and NKG2D), and the activation of NF-{kappa}B pathways related with immune response. Our results demonstrated that ATRA suppressed NF-{kappa}B activity and prevented I{kappa}B{alpha} degradation in a dose-dependent way, inhibited IFN-{gamma} production and gene expression of granzyme B and NKp46. Our findings suggest that ATRA is a negative regulator of NK92 cell activation and may act as a potential regulator of anti-inflammatory functions in vivo.},
doi = {},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 352,
place = {United States},
year = {Fri Jan 05 00:00:00 EST 2007},
month = {Fri Jan 05 00:00:00 EST 2007}
}