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Title: Characterization of CD133{sup +} hepatocellular carcinoma cells as cancer stem/progenitor cells

Abstract

The CD133 antigen, identified as a hematopoietic stem cell marker, appears in various human embryonic epithelia including the neural tube, gut, and kidney. We herein investigated whether CD133{sup +} cells isolated from human hepatocellular carcinoma cell lines possess cancer stem/progenitor cell-like properties. Among the three cell lines studied, the CD133 antigen was found to be expressed only on the surface of Huh-7 cells. CD133{sup +} cells from Huh-7 performed a higher in vitro proliferative potential and lower mRNA expressions of mature hepatocyte markers, glutamine synthetase and cytochrome P450 3A4, than CD133{sup -} population of Huh-7 cells. When either CD133{sup +} or CD133{sup -} cells were subcutaneously injected into SCID mice, CD133{sup +} cells formed tumors, whereas CD133{sup -} cells induced either a very small number of tumors or none at all. Taken together, the identification of CD133{sup +} cells could thus be a potentially powerful tool to investigate the tumorigenic process in the hepatoma system and to also develop effective therapies targeted against hepatocellular carcinoma.

Authors:
 [1];  [2];  [3];  [3];  [3];  [1]
  1. Department of Gastroenterology, Gifu University Graduate School of Medicine, Gifu 501 1194 (Japan)
  2. Department of Gastroenterology, Gifu University Graduate School of Medicine, Gifu 501 1194 (Japan). E-mail: mnagaki@cc.gifu-u.ac.jp
  3. Department of Tissue and Organ Development, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Gifu 501 1194 (Japan)
Publication Date:
OSTI Identifier:
20857933
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 351; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2006.10.128; PII: S0006-291X(06)02324-2; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIGENS; GLUTAMINE; HEPATOMAS; IN VITRO; KIDNEYS; LIGASES; MICE; STEM CELLS; THERAPY

Citation Formats

Suetsugu, Atsushi, Nagaki, Masahito, Aoki, Hitomi, Motohashi, Tsutomu, Kunisada, Takahiro, and Moriwaki, Hisataka. Characterization of CD133{sup +} hepatocellular carcinoma cells as cancer stem/progenitor cells. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2006.10.128.
Suetsugu, Atsushi, Nagaki, Masahito, Aoki, Hitomi, Motohashi, Tsutomu, Kunisada, Takahiro, & Moriwaki, Hisataka. Characterization of CD133{sup +} hepatocellular carcinoma cells as cancer stem/progenitor cells. United States. doi:10.1016/j.bbrc.2006.10.128.
Suetsugu, Atsushi, Nagaki, Masahito, Aoki, Hitomi, Motohashi, Tsutomu, Kunisada, Takahiro, and Moriwaki, Hisataka. Fri . "Characterization of CD133{sup +} hepatocellular carcinoma cells as cancer stem/progenitor cells". United States. doi:10.1016/j.bbrc.2006.10.128.
@article{osti_20857933,
title = {Characterization of CD133{sup +} hepatocellular carcinoma cells as cancer stem/progenitor cells},
author = {Suetsugu, Atsushi and Nagaki, Masahito and Aoki, Hitomi and Motohashi, Tsutomu and Kunisada, Takahiro and Moriwaki, Hisataka},
abstractNote = {The CD133 antigen, identified as a hematopoietic stem cell marker, appears in various human embryonic epithelia including the neural tube, gut, and kidney. We herein investigated whether CD133{sup +} cells isolated from human hepatocellular carcinoma cell lines possess cancer stem/progenitor cell-like properties. Among the three cell lines studied, the CD133 antigen was found to be expressed only on the surface of Huh-7 cells. CD133{sup +} cells from Huh-7 performed a higher in vitro proliferative potential and lower mRNA expressions of mature hepatocyte markers, glutamine synthetase and cytochrome P450 3A4, than CD133{sup -} population of Huh-7 cells. When either CD133{sup +} or CD133{sup -} cells were subcutaneously injected into SCID mice, CD133{sup +} cells formed tumors, whereas CD133{sup -} cells induced either a very small number of tumors or none at all. Taken together, the identification of CD133{sup +} cells could thus be a potentially powerful tool to investigate the tumorigenic process in the hepatoma system and to also develop effective therapies targeted against hepatocellular carcinoma.},
doi = {10.1016/j.bbrc.2006.10.128},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 351,
place = {United States},
year = {Fri Dec 29 00:00:00 EST 2006},
month = {Fri Dec 29 00:00:00 EST 2006}
}