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Title: Combination of integrin siRNA and irradiation for breast cancer therapy

Abstract

Up-regulation of integrin {alpha}{sub v}{beta}{sub 3} has been shown to play a key role in tumor angiogenesis and metastasis. In this study, we evaluated the role of integrin {alpha}{sub v}{beta}{sub 3} in breast cancer cell resistance to ionizing irradiation (IR) and tested the anti-tumor efficacy of combining integrin {alpha}{sub v} siRNA and IR. Colonogenic survival assay, cell proliferation, apoptosis, and cell cycle analysis were carried out to determine the treatment effect of siRNA, IR, or combination of both on MDA-MB-435 cells (integrin {alpha}{sub v}{beta}{sub 3}-positive). Integrin {alpha}{sub v}{beta}{sub 3}-negative MCF-7 cells exert more radiosensitivity than MDA-MB-435 cells. IR up-regulates integrin {alpha}{sub v}{beta}{sub 3} expression in MDA-MB-435 cells and integrin {alpha}{sub v} siRNA can effectively reduce both {alpha}{sub v} and {alpha}{sub v}{beta}{sub 3} integrin expression, leading to increased radiosensitivity. Integrin {alpha}{sub v} siRNA also promotes IR-induced apoptosis and enhances IR-induced G2/M arrest in cell cycle progression. This study, with further optimization, may provide a simple and highly efficient treatment strategy for breast cancer as well as other integrin {alpha}{sub v}{beta}{sub 3}-positive cancer types.

Authors:
 [1];  [1];  [2];  [2];  [1];  [2];  [3]
  1. Molecular Imaging Program at Stanford (MIPS) and Bio-X, Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 (United States)
  2. Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305 (United States)
  3. Molecular Imaging Program at Stanford (MIPS) and Bio-X, Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305 (United States). E-mail: shawchen@stanford.edu
Publication Date:
OSTI Identifier:
20857930
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 351; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2006.10.100; PII: S0006-291X(06)02360-6; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; APOPTOSIS; CARCINOMAS; CELL CYCLE; CELL PROLIFERATION; IRRADIATION; MAMMARY GLANDS; METASTASES; OPTIMIZATION; RADIOSENSITIVITY; RADIOTHERAPY; RNA

Citation Formats

Cao Qizhen, Cai Weibo, Li Tianfang, Yang Yong, Chen Kai, Xing Lei, and Chen Xiaoyuan. Combination of integrin siRNA and irradiation for breast cancer therapy. United States: N. p., 2006. Web.
Cao Qizhen, Cai Weibo, Li Tianfang, Yang Yong, Chen Kai, Xing Lei, & Chen Xiaoyuan. Combination of integrin siRNA and irradiation for breast cancer therapy. United States.
Cao Qizhen, Cai Weibo, Li Tianfang, Yang Yong, Chen Kai, Xing Lei, and Chen Xiaoyuan. Fri . "Combination of integrin siRNA and irradiation for breast cancer therapy". United States. doi:.
@article{osti_20857930,
title = {Combination of integrin siRNA and irradiation for breast cancer therapy},
author = {Cao Qizhen and Cai Weibo and Li Tianfang and Yang Yong and Chen Kai and Xing Lei and Chen Xiaoyuan},
abstractNote = {Up-regulation of integrin {alpha}{sub v}{beta}{sub 3} has been shown to play a key role in tumor angiogenesis and metastasis. In this study, we evaluated the role of integrin {alpha}{sub v}{beta}{sub 3} in breast cancer cell resistance to ionizing irradiation (IR) and tested the anti-tumor efficacy of combining integrin {alpha}{sub v} siRNA and IR. Colonogenic survival assay, cell proliferation, apoptosis, and cell cycle analysis were carried out to determine the treatment effect of siRNA, IR, or combination of both on MDA-MB-435 cells (integrin {alpha}{sub v}{beta}{sub 3}-positive). Integrin {alpha}{sub v}{beta}{sub 3}-negative MCF-7 cells exert more radiosensitivity than MDA-MB-435 cells. IR up-regulates integrin {alpha}{sub v}{beta}{sub 3} expression in MDA-MB-435 cells and integrin {alpha}{sub v} siRNA can effectively reduce both {alpha}{sub v} and {alpha}{sub v}{beta}{sub 3} integrin expression, leading to increased radiosensitivity. Integrin {alpha}{sub v} siRNA also promotes IR-induced apoptosis and enhances IR-induced G2/M arrest in cell cycle progression. This study, with further optimization, may provide a simple and highly efficient treatment strategy for breast cancer as well as other integrin {alpha}{sub v}{beta}{sub 3}-positive cancer types.},
doi = {},
journal = {Biochemical and Biophysical Research Communications},
number = 3,
volume = 351,
place = {United States},
year = {Fri Dec 22 00:00:00 EST 2006},
month = {Fri Dec 22 00:00:00 EST 2006}
}