Transactivation of the proximal promoter of human oxytocin gene by TR4 orphan receptor
Abstract
The human testicular receptor 4 (TR4) shares structural homology with members of the nuclear receptor superfamily. Some other members of this superfamily were able to regulate the transcriptional activity of the human oxytocin (OXT) promoter by binding to the first DR0 regulatory site. However, little investigation was conducted systematically in the study of the second dDR4 site of OXT proximal promoter, and the relationship between the first and the second sites of OXT promoter. Here, we demonstrated for the first time that TR4 could increase the proximal promoter activity of the human OXT gene via DR0, dDR4, and OXT (both DR0 and dDR4) elements, respectively. TR4 might induce OXT gene expression through the OXT element in a dose-dependent manner. However, there is no synergistic effect between DR0 and dDR4 elements during TR4 transactivation. Taken together, these results suggested that TR4 should be one of important regulators of OXT gene expression.
- Authors:
-
- Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan (China)
- George Whipple Laboratory for Cancer Research, Department of Urology, University of Rochester Medical Center, Rochester, NY 14642 (United States)
- Publication Date:
- OSTI Identifier:
- 20857906
- Resource Type:
- Journal Article
- Journal Name:
- Biochemical and Biophysical Research Communications
- Additional Journal Information:
- Journal Volume: 351; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2006.10.021; PII: S0006-291X(06)02254-6; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; GENE REGULATION; GENES; OXYTOCIN; PROMOTERS; RECEPTORS; TESTES
Citation Formats
Wang, C -P, Lee, Y -F, Chang, C, and Lee, H -J. Transactivation of the proximal promoter of human oxytocin gene by TR4 orphan receptor. United States: N. p., 2006.
Web. doi:10.1016/j.bbrc.2006.10.021.
Wang, C -P, Lee, Y -F, Chang, C, & Lee, H -J. Transactivation of the proximal promoter of human oxytocin gene by TR4 orphan receptor. United States. https://doi.org/10.1016/j.bbrc.2006.10.021
Wang, C -P, Lee, Y -F, Chang, C, and Lee, H -J. 2006.
"Transactivation of the proximal promoter of human oxytocin gene by TR4 orphan receptor". United States. https://doi.org/10.1016/j.bbrc.2006.10.021.
@article{osti_20857906,
title = {Transactivation of the proximal promoter of human oxytocin gene by TR4 orphan receptor},
author = {Wang, C -P and Lee, Y -F and Chang, C and Lee, H -J},
abstractNote = {The human testicular receptor 4 (TR4) shares structural homology with members of the nuclear receptor superfamily. Some other members of this superfamily were able to regulate the transcriptional activity of the human oxytocin (OXT) promoter by binding to the first DR0 regulatory site. However, little investigation was conducted systematically in the study of the second dDR4 site of OXT proximal promoter, and the relationship between the first and the second sites of OXT promoter. Here, we demonstrated for the first time that TR4 could increase the proximal promoter activity of the human OXT gene via DR0, dDR4, and OXT (both DR0 and dDR4) elements, respectively. TR4 might induce OXT gene expression through the OXT element in a dose-dependent manner. However, there is no synergistic effect between DR0 and dDR4 elements during TR4 transactivation. Taken together, these results suggested that TR4 should be one of important regulators of OXT gene expression.},
doi = {10.1016/j.bbrc.2006.10.021},
url = {https://www.osti.gov/biblio/20857906},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 351,
place = {United States},
year = {Fri Dec 08 00:00:00 EST 2006},
month = {Fri Dec 08 00:00:00 EST 2006}
}