Nuclear interaction of Smac/DIABLO with Survivin at G2/M arrest prompts docetaxel-induced apoptosis in DU145 prostate cancer cells

Journal Article · Fri Dec 01 00:00:00 EST 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.09.143· OSTI ID:20854599

Kim, Ji Young ^[1]; Chung, Jin-Yong ^[1]; Lee, Seung Gee ^[1]; Kim, Yoon-Jae ^[1]; Park, Ji-Eun ^[1]; Yoo, Ki Soo ^[1]; Yoo, Young Hyun ^[1]; Park, Young Chul ^[2]; Kim, Byeong Gee ^[3]; Kim, Jong-Min ^[1]

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1. Department of Anatomy and Cell Biology, College of Medicine, Dong-A University, Busan 602-714 (Korea, Republic of)
2. Department of Microbiology and Immunology, Pusan National University School of Medicine, Busan 602-739 (Korea, Republic of)
3. Department of Biology, College of Natural Sciences, Pusan National University, Busan 609-735 (Korea, Republic of)

Smac/DIABLO is released by mitochondria in response to apoptotic stimuli and is thought to antagonize the function of inhibitors of apoptosis proteins. Recently, it has been shown that, like XIAP, Survivin can potentially interact with Smac/DIABLO. However, the precise mechanisms and cellular location of their action have not been determined. We report for the first time that Smac/DIABLO translocates to the nucleus and is colocalized with Survivin at mitotic spindles during apoptosis resulting from G2/M arrest due to docetaxel treatment of DU145 prostate cancer cells. Our data demonstrate that the nuclear interaction of Smac/DIABLO with Survivin is an important step for suppressing the anti-apoptotic function of Survivin in Doc-induced apoptosis. This suggests that the balance between cellular Smac/DIABLO and Survivin levels could be critical for cellular destiny in taxane-treated cancer cells.

• OSTI ID: 20854599
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 350, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2006.09.143; PII: S0006-291X(06)02180-2; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
APOPTOSIS
MITOCHONDRIA
PROSTATE
PROTEINS
STIMULI