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Title: In vitro evaluation of alginate encapsulated adipose-tissue stromal cells for use as injectable bone graft substitute

Abstract

This study aims to investigate the survival and osteogenic behavior of murine-derived adipose-tissue stromal cells (ATSCs) encapsulated in alginate microcapsules thereby instigating further studies in this cell delivery strategy for in vivo osteogenesis. Cell viability was quantified using a tetrazolium-based assay and osteogenic differentiation was evaluated by both alkaline-phosphatase (ALP) histochemistry and osteocalcin mRNA analysis. Following microencapsulation, cell numbers increased from 3.9 x 10{sup 3} on day 1 to 7.8 x 10{sup 3} on day 7 and maintained excellent viability in the course of 21-day culture. ALP was 6.9, 5.5, and 3.2 times higher than monolayer cultures on days 7, 14, and 21, respectively. In addition, osteocalcin mRNA was detectable in encapsulated cultures earlier (day 14) than monolayer cultures. We conclude that alginate microcapsules can act as three-dimensional matrix for ATSC proliferation and has potential for use as injectable, biodegradable scaffold in bone tissue engineering.

Authors:
 [1];  [2];  [1];  [1];  [3];  [3];  [1];  [1];  [1]
  1. Department of Orthopaedics and Traumatology, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong (China)
  2. Department of Orthopaedics and Traumatology, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong (China) and Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong (China)
  3. Research Institute of Polymeric Materials, Tianjin University, Tianjin (China)
Publication Date:
OSTI Identifier:
20854416
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 347; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2006.06.072; PII: S0006-291X(06)01366-0; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADIPOSE TISSUE; ALGINATES; ALKALINE PHOSPHATASE; BONE TISSUES; CELL PROLIFERATION; GRAFTS; IN VITRO; IN VIVO; SKELETON; TETRAZOLIUM

Citation Formats

Abbah, S A, Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, Lu, W W, Chan, D, Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, Cheung, K M.C., Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, Liu, W G, Zhao, F, Li, Z Y, Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, Leong, J C.Y., Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, Luk, K D.K., and Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong. In vitro evaluation of alginate encapsulated adipose-tissue stromal cells for use as injectable bone graft substitute. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2006.06.072.
Abbah, S A, Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, Lu, W W, Chan, D, Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, Cheung, K M.C., Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, Liu, W G, Zhao, F, Li, Z Y, Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, Leong, J C.Y., Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, Luk, K D.K., & Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong. In vitro evaluation of alginate encapsulated adipose-tissue stromal cells for use as injectable bone graft substitute. United States. https://doi.org/10.1016/j.bbrc.2006.06.072
Abbah, S A, Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, Lu, W W, Chan, D, Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, Cheung, K M.C., Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, Liu, W G, Zhao, F, Li, Z Y, Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, Leong, J C.Y., Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, Luk, K D.K., and Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong. 2006. "In vitro evaluation of alginate encapsulated adipose-tissue stromal cells for use as injectable bone graft substitute". United States. https://doi.org/10.1016/j.bbrc.2006.06.072.
@article{osti_20854416,
title = {In vitro evaluation of alginate encapsulated adipose-tissue stromal cells for use as injectable bone graft substitute},
author = {Abbah, S A and Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong and Lu, W W and Chan, D and Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong and Cheung, K M.C. and Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong and Liu, W G and Zhao, F and Li, Z Y and Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong and Leong, J C.Y. and Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong and Luk, K D.K. and Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong},
abstractNote = {This study aims to investigate the survival and osteogenic behavior of murine-derived adipose-tissue stromal cells (ATSCs) encapsulated in alginate microcapsules thereby instigating further studies in this cell delivery strategy for in vivo osteogenesis. Cell viability was quantified using a tetrazolium-based assay and osteogenic differentiation was evaluated by both alkaline-phosphatase (ALP) histochemistry and osteocalcin mRNA analysis. Following microencapsulation, cell numbers increased from 3.9 x 10{sup 3} on day 1 to 7.8 x 10{sup 3} on day 7 and maintained excellent viability in the course of 21-day culture. ALP was 6.9, 5.5, and 3.2 times higher than monolayer cultures on days 7, 14, and 21, respectively. In addition, osteocalcin mRNA was detectable in encapsulated cultures earlier (day 14) than monolayer cultures. We conclude that alginate microcapsules can act as three-dimensional matrix for ATSC proliferation and has potential for use as injectable, biodegradable scaffold in bone tissue engineering.},
doi = {10.1016/j.bbrc.2006.06.072},
url = {https://www.osti.gov/biblio/20854416}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 347,
place = {United States},
year = {Fri Aug 18 00:00:00 EDT 2006},
month = {Fri Aug 18 00:00:00 EDT 2006}
}