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Title: The flavonoid quercetin induces apoptosis and inhibits JNK activation in intimal vascular smooth muscle cells

Abstract

Quercetin, the most abundant dietary flavonol, exerts vasodilator, anti-hypertensive, and anti-atherogenic effects and reduces the vascular remodelling associated with elevated blood pressure. Here, we have compared the effects of quercetin in intimal- and medial-type rat vascular smooth muscle cells (VSMC) in culture. After 48 h, quercetin reduced the viability of a polyclonal intimal-type cell line derived from neonatal aorta but not of a medial-type cell line derived from adult aorta. These differential effects were similar in both proliferating and quiescent VSMC. Quercetin also preferentially reduced the viability of intimal-type over medial-type VSMC in primary cultures derived from balloon-injured carotid arteries. The effects of quercetin on cell viability were mainly dependent upon induction of apoptosis, as demonstrated by nuclear condensation and fragmentation, and were unrelated to PPAR{gamma}, pro-oxidant effects or nitric oxide. The expression of MAPKs (ERK, p38, and JNK) and ERK phosphorylation were not different between intimal- and medial-type VSMC. p38 phosphorylation was negligible in both cell types. Medial-type showed a weak JNK phosphorylation while this was markedly increased in intimal-type cells. Quercetin reduced JNK phosphorylation but had no consistent effect on ERK phosphorylation. In conclusion, quercetin preferentially produced apoptosis in intimal-type compared to medial-type VSMC. This might play amore » role in the anti-atherogenic and anti-hypertensive effects of quercetin.« less

Authors:
 [1];  [2];  [3];  [4];  [3];  [3];  [5];  [6];  [2]
  1. Cardiac, Vascular and Inflammation Research, William Harvey Research Institute, Queen Mary University of London (United Kingdom) and Department of Pharmacology, School of Medicine, Universidad Complutense de Madrid (Spain). E-mail: fperez@med.ucm.es
  2. Cardiac, Vascular and Inflammation Research, William Harvey Research Institute, Queen Mary University of London (United Kingdom)
  3. Department of Pharmacology, School of Medicine, Universidad Complutense de Madrid (Spain)
  4. Department of Pharmacology, School of Pharmacy, Universidad de Granada (Spain)
  5. Centro Nacional de Investigaciones Cardiovasculares, Madrid (Spain)
  6. National Heart and Lung Institute, Imperial College School of Medicine, London (United Kingdom)
Publication Date:
OSTI Identifier:
20854394
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 346; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2006.05.198; PII: S0006-291X(06)01281-2; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AORTA; APOPTOSIS; BLOOD PRESSURE; CAROTID ARTERIES; MUSCLES; NITRIC OXIDE; OXIDIZERS; PHOSPHORYLATION; QUERCETIN; RATS; VASODILATORS

Citation Formats

Perez-Vizcaino, Francisco, Bishop-Bailley, David, Lodi, Federica, Duarte, Juan, Cogolludo, Angel, Moreno, Laura, Bosca, Lisardo, Mitchell, Jane A., and Warner, Timothy D.. The flavonoid quercetin induces apoptosis and inhibits JNK activation in intimal vascular smooth muscle cells. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2006.05.198.
Perez-Vizcaino, Francisco, Bishop-Bailley, David, Lodi, Federica, Duarte, Juan, Cogolludo, Angel, Moreno, Laura, Bosca, Lisardo, Mitchell, Jane A., & Warner, Timothy D.. The flavonoid quercetin induces apoptosis and inhibits JNK activation in intimal vascular smooth muscle cells. United States. doi:10.1016/j.bbrc.2006.05.198.
Perez-Vizcaino, Francisco, Bishop-Bailley, David, Lodi, Federica, Duarte, Juan, Cogolludo, Angel, Moreno, Laura, Bosca, Lisardo, Mitchell, Jane A., and Warner, Timothy D.. Fri . "The flavonoid quercetin induces apoptosis and inhibits JNK activation in intimal vascular smooth muscle cells". United States. doi:10.1016/j.bbrc.2006.05.198.
@article{osti_20854394,
title = {The flavonoid quercetin induces apoptosis and inhibits JNK activation in intimal vascular smooth muscle cells},
author = {Perez-Vizcaino, Francisco and Bishop-Bailley, David and Lodi, Federica and Duarte, Juan and Cogolludo, Angel and Moreno, Laura and Bosca, Lisardo and Mitchell, Jane A. and Warner, Timothy D.},
abstractNote = {Quercetin, the most abundant dietary flavonol, exerts vasodilator, anti-hypertensive, and anti-atherogenic effects and reduces the vascular remodelling associated with elevated blood pressure. Here, we have compared the effects of quercetin in intimal- and medial-type rat vascular smooth muscle cells (VSMC) in culture. After 48 h, quercetin reduced the viability of a polyclonal intimal-type cell line derived from neonatal aorta but not of a medial-type cell line derived from adult aorta. These differential effects were similar in both proliferating and quiescent VSMC. Quercetin also preferentially reduced the viability of intimal-type over medial-type VSMC in primary cultures derived from balloon-injured carotid arteries. The effects of quercetin on cell viability were mainly dependent upon induction of apoptosis, as demonstrated by nuclear condensation and fragmentation, and were unrelated to PPAR{gamma}, pro-oxidant effects or nitric oxide. The expression of MAPKs (ERK, p38, and JNK) and ERK phosphorylation were not different between intimal- and medial-type VSMC. p38 phosphorylation was negligible in both cell types. Medial-type showed a weak JNK phosphorylation while this was markedly increased in intimal-type cells. Quercetin reduced JNK phosphorylation but had no consistent effect on ERK phosphorylation. In conclusion, quercetin preferentially produced apoptosis in intimal-type compared to medial-type VSMC. This might play a role in the anti-atherogenic and anti-hypertensive effects of quercetin.},
doi = {10.1016/j.bbrc.2006.05.198},
journal = {Biochemical and Biophysical Research Communications},
number = 3,
volume = 346,
place = {United States},
year = {Fri Aug 04 00:00:00 EDT 2006},
month = {Fri Aug 04 00:00:00 EDT 2006}
}