skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Immunogenicity of West Nile virus infectious DNA and its noninfectious derivatives

Abstract

The exceptionally high virulence of the West Nile NY99 strain makes its suitability in the development of a live WN vaccine uncertain. The aim of this study is to investigate the immunogenicity of noninfectious virus derivatives carrying pseudolethal mutations, which preclude virion formation without affecting preceding steps of the viral infectious cycle. When administered using DNA immunization, such constructs initiate an infectious cycle but cannot lead to a viremia. While the magnitude of the immune response to a noninfectious replication-competent construct was lower than that of virus or infectious DNA, its overall quality and the protective effect were similar. In contrast, a nonreplicating construct of similar length induced only a marginally detectable immune response in the dose range used. Thus, replication-competent noninfectious constructs derived from infectious DNA may offer an advantageous combination of the safety of noninfectious formulations with the quality of the immune response characteristic of infectious vaccines.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [2]
  1. Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045 (United States)
  2. Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045 (United States). E-mail: yaximik@ku.edu
Publication Date:
OSTI Identifier:
20850580
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 356; Journal Issue: 1-2; Other Information: DOI: 10.1016/j.virol.2006.07.038; PII: S0042-6822(06)00517-4; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; DNA; IMMUNE REACTIONS; MICE; MUTATIONS; VACCINES; VIRULENCE; VIRUSES

Citation Formats

Seregin, Alexey, Nistler, Ryan, Borisevich, Victoria, Yamshchikov, Galina, Chaporgina, Elena, Kwok, Chun Wai, and Yamshchikov, Vladimir. Immunogenicity of West Nile virus infectious DNA and its noninfectious derivatives. United States: N. p., 2006. Web. doi:10.1016/j.virol.2006.07.038.
Seregin, Alexey, Nistler, Ryan, Borisevich, Victoria, Yamshchikov, Galina, Chaporgina, Elena, Kwok, Chun Wai, & Yamshchikov, Vladimir. Immunogenicity of West Nile virus infectious DNA and its noninfectious derivatives. United States. doi:10.1016/j.virol.2006.07.038.
Seregin, Alexey, Nistler, Ryan, Borisevich, Victoria, Yamshchikov, Galina, Chaporgina, Elena, Kwok, Chun Wai, and Yamshchikov, Vladimir. Wed . "Immunogenicity of West Nile virus infectious DNA and its noninfectious derivatives". United States. doi:10.1016/j.virol.2006.07.038.
@article{osti_20850580,
title = {Immunogenicity of West Nile virus infectious DNA and its noninfectious derivatives},
author = {Seregin, Alexey and Nistler, Ryan and Borisevich, Victoria and Yamshchikov, Galina and Chaporgina, Elena and Kwok, Chun Wai and Yamshchikov, Vladimir},
abstractNote = {The exceptionally high virulence of the West Nile NY99 strain makes its suitability in the development of a live WN vaccine uncertain. The aim of this study is to investigate the immunogenicity of noninfectious virus derivatives carrying pseudolethal mutations, which preclude virion formation without affecting preceding steps of the viral infectious cycle. When administered using DNA immunization, such constructs initiate an infectious cycle but cannot lead to a viremia. While the magnitude of the immune response to a noninfectious replication-competent construct was lower than that of virus or infectious DNA, its overall quality and the protective effect were similar. In contrast, a nonreplicating construct of similar length induced only a marginally detectable immune response in the dose range used. Thus, replication-competent noninfectious constructs derived from infectious DNA may offer an advantageous combination of the safety of noninfectious formulations with the quality of the immune response characteristic of infectious vaccines.},
doi = {10.1016/j.virol.2006.07.038},
journal = {Virology},
number = 1-2,
volume = 356,
place = {United States},
year = {Wed Dec 20 00:00:00 EST 2006},
month = {Wed Dec 20 00:00:00 EST 2006}
}