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Title: Binding of the polypyrimidine tract-binding protein-associated splicing factor (PSF) to the hepatitis delta virus RNA

Abstract

The hepatitis delta virus (HDV) has a very limited protein coding capacity and must rely on host proteins for its replication. A ribonucleoprotein complex was detected following UV cross-linking between HeLa nuclear proteins and an RNA corresponding to the right terminal stem-loop domain of HDV genomic RNA. Mass spectrometric analysis of the complex revealed the polypyrimidine tract-binding protein-associated splicing factor (PSF) as a novel HDV RNA-interacting protein. Co-immunoprecipitation demonstrated the interaction between HDV RNA and PSF both in vitro in HeLa nuclear extract and in vivo within HeLa cells containing both polarities of the HDV genome. Analysis of the binding of various HDV-derived RNAs to purified, recombinant PSF further confirmed the specificity of the interaction and revealed that PSF directly binds to the terminal stem-loop domains of both polarities of HDV RNA. Our findings provide evidence of the involvement of a host mRNA processing protein in the HDV life cycle.

Authors:
 [1];  [1];  [2]
  1. Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Room 4223A, Ottawa, ON, K1H 8M5 (Canada)
  2. Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Room 4223A, Ottawa, ON, K1H 8M5 (Canada). E-mail: mpelchat@uottawa.ca
Publication Date:
OSTI Identifier:
20850578
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 356; Journal Issue: 1-2; Other Information: DOI: 10.1016/j.virol.2006.06.040; PII: S0042-6822(06)00516-2; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CROSS-LINKING; HELA CELLS; HEPATITIS; IN VITRO; IN VIVO; LIFE CYCLE; MASS SPECTROSCOPY; PROTEINS; RNA; SPECIFICITY; SPLICING; VIRUSES

Citation Formats

Greco-Stewart, Valerie S., Thibault, Catherine St-Laurent, and Pelchat, Martin. Binding of the polypyrimidine tract-binding protein-associated splicing factor (PSF) to the hepatitis delta virus RNA. United States: N. p., 2006. Web. doi:10.1016/j.virol.2006.06.040.
Greco-Stewart, Valerie S., Thibault, Catherine St-Laurent, & Pelchat, Martin. Binding of the polypyrimidine tract-binding protein-associated splicing factor (PSF) to the hepatitis delta virus RNA. United States. doi:10.1016/j.virol.2006.06.040.
Greco-Stewart, Valerie S., Thibault, Catherine St-Laurent, and Pelchat, Martin. Wed . "Binding of the polypyrimidine tract-binding protein-associated splicing factor (PSF) to the hepatitis delta virus RNA". United States. doi:10.1016/j.virol.2006.06.040.
@article{osti_20850578,
title = {Binding of the polypyrimidine tract-binding protein-associated splicing factor (PSF) to the hepatitis delta virus RNA},
author = {Greco-Stewart, Valerie S. and Thibault, Catherine St-Laurent and Pelchat, Martin},
abstractNote = {The hepatitis delta virus (HDV) has a very limited protein coding capacity and must rely on host proteins for its replication. A ribonucleoprotein complex was detected following UV cross-linking between HeLa nuclear proteins and an RNA corresponding to the right terminal stem-loop domain of HDV genomic RNA. Mass spectrometric analysis of the complex revealed the polypyrimidine tract-binding protein-associated splicing factor (PSF) as a novel HDV RNA-interacting protein. Co-immunoprecipitation demonstrated the interaction between HDV RNA and PSF both in vitro in HeLa nuclear extract and in vivo within HeLa cells containing both polarities of the HDV genome. Analysis of the binding of various HDV-derived RNAs to purified, recombinant PSF further confirmed the specificity of the interaction and revealed that PSF directly binds to the terminal stem-loop domains of both polarities of HDV RNA. Our findings provide evidence of the involvement of a host mRNA processing protein in the HDV life cycle.},
doi = {10.1016/j.virol.2006.06.040},
journal = {Virology},
number = 1-2,
volume = 356,
place = {United States},
year = {Wed Dec 20 00:00:00 EST 2006},
month = {Wed Dec 20 00:00:00 EST 2006}
}