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Title: Characterization of the CD8{sup +} T cell responses directed against respiratory syncytial virus during primary and secondary infection in C57BL/6 mice

Abstract

The BALB/c mouse model for human respiratory syncytial virus infection has contributed significantly to our understanding of the relative role for CD4{sup +} and CD8{sup +} T cells to immune protection and pathogenic immune responses. To enable comparison of RSV-specific T cell responses in different mouse strains and allow dissection of immune mechanisms by using transgenic and knockout mice that are mostly available on a C57BL/6 background, we characterized the specificity, level and functional capabilities of CD8{sup +} T cells during primary and secondary responses in lung parenchyma, airways and spleens of C57BL/6 mice. During the primary response, epitopes were recognized originating from the matrix, fusion, nucleo- and attachment proteins, whereas the secondary response focused predominantly on the matrix epitope. C57BL/6 mice are less permissive for hRSV infection than BALB/c mice, yet we found CD8{sup +} T cell responses in the lungs and bronchoalveolar lavage, comparable to the responses described for BALB/c mice.

Authors:
 [1];  [2];  [1];  [1];  [3];  [4];  [4];  [2];  [1];  [5]
  1. Department of Pediatrics, Wilhelmina Children's Hospital, University Medical Center, KE.04.133.1, Lundlaan 6, 3584 EA Utrecht (Netherlands)
  2. Department of Immunology, Faculty of Veterinary Science, University of Utrecht, Utrecht (Netherlands)
  3. National Vaccine Institute, Bilthoven (Netherlands)
  4. Division of Immunology, Netherlands Cancer Institute, Amsterdam (Netherlands)
  5. Department of Pediatrics, Wilhelmina Children's Hospital, University Medical Center, KE.04.133.1, Lundlaan 6, 3584 EA Utrecht (Netherlands). E-mail: g.vanbleek@umcutrecht.nl
Publication Date:
OSTI Identifier:
20850547
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 352; Journal Issue: 1; Other Information: DOI: 10.1016/j.virol.2006.04.023; PII: S0042-6822(06)00273-X; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; INACTIVATION; KNOCK-OUT REACTIONS; LUNGS; MICE; PROTEINS; SPLEEN; VIRUSES

Citation Formats

Lukens, Michael V., Claassen, Erwin A.W., Graaff, Patricia M.A. de, Dijk, Mariska E.A. van, Hoogerhout, Peter, Toebes, Mireille, Schumacher, Ton N., Most, Robbert G. van der, Kimpen, Jan L.L., and Bleek, Grada M. van. Characterization of the CD8{sup +} T cell responses directed against respiratory syncytial virus during primary and secondary infection in C57BL/6 mice. United States: N. p., 2006. Web. doi:10.1016/j.virol.2006.04.023.
Lukens, Michael V., Claassen, Erwin A.W., Graaff, Patricia M.A. de, Dijk, Mariska E.A. van, Hoogerhout, Peter, Toebes, Mireille, Schumacher, Ton N., Most, Robbert G. van der, Kimpen, Jan L.L., & Bleek, Grada M. van. Characterization of the CD8{sup +} T cell responses directed against respiratory syncytial virus during primary and secondary infection in C57BL/6 mice. United States. doi:10.1016/j.virol.2006.04.023.
Lukens, Michael V., Claassen, Erwin A.W., Graaff, Patricia M.A. de, Dijk, Mariska E.A. van, Hoogerhout, Peter, Toebes, Mireille, Schumacher, Ton N., Most, Robbert G. van der, Kimpen, Jan L.L., and Bleek, Grada M. van. Tue . "Characterization of the CD8{sup +} T cell responses directed against respiratory syncytial virus during primary and secondary infection in C57BL/6 mice". United States. doi:10.1016/j.virol.2006.04.023.
@article{osti_20850547,
title = {Characterization of the CD8{sup +} T cell responses directed against respiratory syncytial virus during primary and secondary infection in C57BL/6 mice},
author = {Lukens, Michael V. and Claassen, Erwin A.W. and Graaff, Patricia M.A. de and Dijk, Mariska E.A. van and Hoogerhout, Peter and Toebes, Mireille and Schumacher, Ton N. and Most, Robbert G. van der and Kimpen, Jan L.L. and Bleek, Grada M. van},
abstractNote = {The BALB/c mouse model for human respiratory syncytial virus infection has contributed significantly to our understanding of the relative role for CD4{sup +} and CD8{sup +} T cells to immune protection and pathogenic immune responses. To enable comparison of RSV-specific T cell responses in different mouse strains and allow dissection of immune mechanisms by using transgenic and knockout mice that are mostly available on a C57BL/6 background, we characterized the specificity, level and functional capabilities of CD8{sup +} T cells during primary and secondary responses in lung parenchyma, airways and spleens of C57BL/6 mice. During the primary response, epitopes were recognized originating from the matrix, fusion, nucleo- and attachment proteins, whereas the secondary response focused predominantly on the matrix epitope. C57BL/6 mice are less permissive for hRSV infection than BALB/c mice, yet we found CD8{sup +} T cell responses in the lungs and bronchoalveolar lavage, comparable to the responses described for BALB/c mice.},
doi = {10.1016/j.virol.2006.04.023},
journal = {Virology},
number = 1,
volume = 352,
place = {United States},
year = {Tue Aug 15 00:00:00 EDT 2006},
month = {Tue Aug 15 00:00:00 EDT 2006}
}