Characterization of the CD8{sup +} T cell responses directed against respiratory syncytial virus during primary and secondary infection in C57BL/6 mice
- Department of Pediatrics, Wilhelmina Children's Hospital, University Medical Center, KE.04.133.1, Lundlaan 6, 3584 EA Utrecht (Netherlands)
- Department of Immunology, Faculty of Veterinary Science, University of Utrecht, Utrecht (Netherlands)
- National Vaccine Institute, Bilthoven (Netherlands)
- Division of Immunology, Netherlands Cancer Institute, Amsterdam (Netherlands)
The BALB/c mouse model for human respiratory syncytial virus infection has contributed significantly to our understanding of the relative role for CD4{sup +} and CD8{sup +} T cells to immune protection and pathogenic immune responses. To enable comparison of RSV-specific T cell responses in different mouse strains and allow dissection of immune mechanisms by using transgenic and knockout mice that are mostly available on a C57BL/6 background, we characterized the specificity, level and functional capabilities of CD8{sup +} T cells during primary and secondary responses in lung parenchyma, airways and spleens of C57BL/6 mice. During the primary response, epitopes were recognized originating from the matrix, fusion, nucleo- and attachment proteins, whereas the secondary response focused predominantly on the matrix epitope. C57BL/6 mice are less permissive for hRSV infection than BALB/c mice, yet we found CD8{sup +} T cell responses in the lungs and bronchoalveolar lavage, comparable to the responses described for BALB/c mice.
- OSTI ID:
- 20850547
- Journal Information:
- Virology, Vol. 352, Issue 1; Other Information: DOI: 10.1016/j.virol.2006.04.023; PII: S0042-6822(06)00273-X; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
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