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Title: Structural and functional localization of airway effects from episodic exposure of infant monkeys to allergen and/or ozone

Abstract

Both allergen and ozone exposure increase asthma symptoms and airway responsiveness in children. Little is known about how these inhalants may differentially modify airway responsiveness in large proximal as compared to small distal airways. We evaluated whether bronchi and respiratory bronchioles from infant monkeys exposed episodically to allergen and/or ozone differentially develop intrinsic hyperresponsiveness to methacholine and whether eosinophils and/or pulmonary neuroendocrine cells play a role. Infant monkeys were exposed episodically for 5 months to: (1) filtered air, (2) aerosolized house dust mite allergen, (3) ozone 0.5 ppm, or (4) house dust mite allergen + ozone. Studying the function/structure relationship of the same lung slices, we evaluated methacholine airway responsiveness and histology of bronchi and respiratory bronchioles. In bronchi, intrinsic responsiveness was increased by allergen exposure, an effect reduced by bombesin antagonist. In respiratory bronchioles, intrinsic airway responsiveness was increased by allergen + ozone exposure. Eosinophils were increased by allergen and allergen + ozone exposure in bronchi and by allergen exposure in respiratory bronchioles. In both airways, exposure to allergen + ozone resulted in fewer tissue eosinophils than did allergen exposure alone. In bronchi, but not in respiratory bronchioles, the number of eosinophils and neuroendocrine cells correlated with airway responsiveness.more » We conclude that episodically exposing infant monkeys to house dust mite allergen with or without ozone increased intrinsic airway responsiveness to methacholine in bronchi differently than in respiratory bronchioles. In bronchi, eosinophils and neuroendocrine cells may play a role in the development of airway hyperresponsiveness.« less

Authors:
 [1];  [2];  [2];  [3];  [3];  [3];  [4];  [3]
  1. Department of Pediatrics, School of Medicine, University of California, Davis, 2516 Stockton Boulevard, Sacramento, CA 95817 (United States). E-mail: jesse.joad@ucdmc.ucdavis.edu
  2. Department of Pediatrics, School of Medicine, University of California, Davis, 2516 Stockton Boulevard, Sacramento, CA 95817 (United States)
  3. Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, CA 95616 (United States)
  4. Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616 (United States)
Publication Date:
OSTI Identifier:
20850377
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 214; Journal Issue: 3; Other Information: DOI: 10.1016/j.taap.2005.12.012; PII: S0041-008X(06)00006-8; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ASTHMA; BRONCHI; DUSTS; EOSINOPHILS; HISTOLOGY; INFANTS; LUNGS; MITES; MONKEYS; OZONE; SYMPTOMS

Citation Formats

Joad, Jesse P., Kott, Kayleen S., Bric, John M., Peake, Janice L., Plopper, Charles G., Schelegle, Edward S., Gershwin, Laurel J., and Pinkerton, Kent E. Structural and functional localization of airway effects from episodic exposure of infant monkeys to allergen and/or ozone. United States: N. p., 2006. Web. doi:10.1016/j.taap.2005.12.012.
Joad, Jesse P., Kott, Kayleen S., Bric, John M., Peake, Janice L., Plopper, Charles G., Schelegle, Edward S., Gershwin, Laurel J., & Pinkerton, Kent E. Structural and functional localization of airway effects from episodic exposure of infant monkeys to allergen and/or ozone. United States. doi:10.1016/j.taap.2005.12.012.
Joad, Jesse P., Kott, Kayleen S., Bric, John M., Peake, Janice L., Plopper, Charles G., Schelegle, Edward S., Gershwin, Laurel J., and Pinkerton, Kent E. Tue . "Structural and functional localization of airway effects from episodic exposure of infant monkeys to allergen and/or ozone". United States. doi:10.1016/j.taap.2005.12.012.
@article{osti_20850377,
title = {Structural and functional localization of airway effects from episodic exposure of infant monkeys to allergen and/or ozone},
author = {Joad, Jesse P. and Kott, Kayleen S. and Bric, John M. and Peake, Janice L. and Plopper, Charles G. and Schelegle, Edward S. and Gershwin, Laurel J. and Pinkerton, Kent E.},
abstractNote = {Both allergen and ozone exposure increase asthma symptoms and airway responsiveness in children. Little is known about how these inhalants may differentially modify airway responsiveness in large proximal as compared to small distal airways. We evaluated whether bronchi and respiratory bronchioles from infant monkeys exposed episodically to allergen and/or ozone differentially develop intrinsic hyperresponsiveness to methacholine and whether eosinophils and/or pulmonary neuroendocrine cells play a role. Infant monkeys were exposed episodically for 5 months to: (1) filtered air, (2) aerosolized house dust mite allergen, (3) ozone 0.5 ppm, or (4) house dust mite allergen + ozone. Studying the function/structure relationship of the same lung slices, we evaluated methacholine airway responsiveness and histology of bronchi and respiratory bronchioles. In bronchi, intrinsic responsiveness was increased by allergen exposure, an effect reduced by bombesin antagonist. In respiratory bronchioles, intrinsic airway responsiveness was increased by allergen + ozone exposure. Eosinophils were increased by allergen and allergen + ozone exposure in bronchi and by allergen exposure in respiratory bronchioles. In both airways, exposure to allergen + ozone resulted in fewer tissue eosinophils than did allergen exposure alone. In bronchi, but not in respiratory bronchioles, the number of eosinophils and neuroendocrine cells correlated with airway responsiveness. We conclude that episodically exposing infant monkeys to house dust mite allergen with or without ozone increased intrinsic airway responsiveness to methacholine in bronchi differently than in respiratory bronchioles. In bronchi, eosinophils and neuroendocrine cells may play a role in the development of airway hyperresponsiveness.},
doi = {10.1016/j.taap.2005.12.012},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 3,
volume = 214,
place = {United States},
year = {2006},
month = {8}
}