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Title: Effect of sulfur dioxide inhalation on CYP2B1/2 and CYP2E1 in rat liver and lung

Abstract

Sulfur dioxide (SO{sub 2}) is a ubiquitous air pollutant, present in low concentrations in the urban air and in higher concentrations in the working environment. In this study, we investigated the effects of inhaled SO{sub 2} on the O-dealkylase of pentoxyresorufin (PROD) and p-nitrophenol hydroxylases (p-NP) activities and mRNA levels of CYP2B1/2 and CYP2E1 in the lung and liver of Wistar rats. Male Wistar rats were housed in exposure chambers and treated with 14.11 {+-}1.53, 28.36 {+-} 2.12, and 56.25 {+-} 4.28 mg /m{sup 3}SO{sub 2} for 6 h/day for 7 days, while control rats were exposed to filtered air in the same condition. The mRNAs of CYP2B1/2 and -2E1 were analyzed in livers and lungs by using reverse-transcription polymerase chain reaction (RT-PCR). Results showed that the PROD activities and mRNA of CYP2B1/2 were decreased in livers and lungs of rats exposed to SO{sub 2}. The p-NP activities and mRNA of CYP2E1 were decreased in lungs but not in livers of rats exposed to SO{sub 2}. Total liver microsomal cytochrome P-450 (CYP) contents were diminished in SO{sub 2} -exposed rats. These results lead to two conclusions: (1) SO{sub 2} exposure can suppress CYP2B1/2 and CYP2E1 in lungs and CYP2B1/2 inmore » livers of rats, thus modifying the liver and lung toxication/detoxication potential, and (2) the total liver microsomal CYP contents were diminished, although the activity and mRNA expression of CYP2E1 in rat livers were not affected by SO{sub 2} exposure.« less

Authors:
;  [1]
  1. Shanxi University, Taiyuan (China). Institute of Environmental Medicine and Toxicology
Publication Date:
OSTI Identifier:
20813173
Resource Type:
Journal Article
Journal Name:
Inhalation Toxicology
Additional Journal Information:
Journal Volume: 18; Journal Issue: 8; Journal ID: ISSN 0895-8378
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; SULFUR DIOXIDE; RATS; ENVIRONMENTAL EXPOSURE; LIVER; INHALATION; LUNGS; CONCENTRATION RATIO; INHIBITION; BIOLOGICAL EFFECTS; NUCLEIC ACIDS; MICROSOMES; ENZYMES; TOXICITY; HEALTH HAZARDS; POLYMERASE CHAIN REACTION; METABOLIC DISEASES; CYTOCHROMES

Citation Formats

Qin, Guohua, and Meng, Ziqiang. Effect of sulfur dioxide inhalation on CYP2B1/2 and CYP2E1 in rat liver and lung. United States: N. p., 2006. Web.
Qin, Guohua, & Meng, Ziqiang. Effect of sulfur dioxide inhalation on CYP2B1/2 and CYP2E1 in rat liver and lung. United States.
Qin, Guohua, and Meng, Ziqiang. 2006. "Effect of sulfur dioxide inhalation on CYP2B1/2 and CYP2E1 in rat liver and lung". United States.
@article{osti_20813173,
title = {Effect of sulfur dioxide inhalation on CYP2B1/2 and CYP2E1 in rat liver and lung},
author = {Qin, Guohua and Meng, Ziqiang},
abstractNote = {Sulfur dioxide (SO{sub 2}) is a ubiquitous air pollutant, present in low concentrations in the urban air and in higher concentrations in the working environment. In this study, we investigated the effects of inhaled SO{sub 2} on the O-dealkylase of pentoxyresorufin (PROD) and p-nitrophenol hydroxylases (p-NP) activities and mRNA levels of CYP2B1/2 and CYP2E1 in the lung and liver of Wistar rats. Male Wistar rats were housed in exposure chambers and treated with 14.11 {+-}1.53, 28.36 {+-} 2.12, and 56.25 {+-} 4.28 mg /m{sup 3}SO{sub 2} for 6 h/day for 7 days, while control rats were exposed to filtered air in the same condition. The mRNAs of CYP2B1/2 and -2E1 were analyzed in livers and lungs by using reverse-transcription polymerase chain reaction (RT-PCR). Results showed that the PROD activities and mRNA of CYP2B1/2 were decreased in livers and lungs of rats exposed to SO{sub 2}. The p-NP activities and mRNA of CYP2E1 were decreased in lungs but not in livers of rats exposed to SO{sub 2}. Total liver microsomal cytochrome P-450 (CYP) contents were diminished in SO{sub 2} -exposed rats. These results lead to two conclusions: (1) SO{sub 2} exposure can suppress CYP2B1/2 and CYP2E1 in lungs and CYP2B1/2 in livers of rats, thus modifying the liver and lung toxication/detoxication potential, and (2) the total liver microsomal CYP contents were diminished, although the activity and mRNA expression of CYP2E1 in rat livers were not affected by SO{sub 2} exposure.},
doi = {},
url = {https://www.osti.gov/biblio/20813173}, journal = {Inhalation Toxicology},
issn = {0895-8378},
number = 8,
volume = 18,
place = {United States},
year = {Sat Jul 15 00:00:00 EDT 2006},
month = {Sat Jul 15 00:00:00 EDT 2006}
}