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Title: A testis-specific and testis developmentally regulated tumor protein D52 (TPD52)-like protein TPD52L3/hD55 interacts with TPD52 family proteins

Abstract

Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast cancer. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. To date, three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. Herein, we identified a novel TPD52-like sequence (TPD52L3, or hD55) in human testis using cDNA microarray. Sequence analysis of the deduced protein suggests that hD55 contains a coiled-coil motif and is highly conserved compared with other TPD52-like sequences. Yeast two-hybrid and GST pull-down assays revealed that hD55 interacts with hD52, hD53, hD54, and itself. cDNA microarray detection found that hD55 was expressed at 5.6-fold higher levels in adult testis than in fetal testis. Additionally, the expression profile shows that hD55 is testis-specific, indicating a potential role for hD55 in testis development and spermatogenesis.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [2];  [1]
  1. Lab of Reproductive Medicine, Department of Cell Biology and Medical Genetics, Nanjing Medical University, Han Zhong Road 140, Nanjing, Jiangsu Province (China)
  2. Department of Breast and Endocrine Surgery, Jiangsu Province Hospital, First Affiliated Hospital of Nanjing Medical University, Guang Zhou Road 300, Nanjing, Jiangsu Province (China)
Publication Date:
OSTI Identifier:
20798990
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 344; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2006.03.208; PII: S0006-291X(06)00783-2; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; CARCINOMAS; CELL PROLIFERATION; MAMMARY GLANDS; PROTEINS; SPERMATOGENESIS; STRUCTURAL CHEMICAL ANALYSIS; YEASTS

Citation Formats

Qinhong, Cao, Department of Breast and Endocrine Surgery, Jiangsu Province Hospital, First Affiliated Hospital of Nanjing Medical University, Guang Zhou Road 300, Nanjing, Jiangsu Province, Jie, Chen, Li, Zhu, Yun, Liu, Zuomin, Zhou, Jiahao, Sha, Shui, Wang, and Jianmin, Li. A testis-specific and testis developmentally regulated tumor protein D52 (TPD52)-like protein TPD52L3/hD55 interacts with TPD52 family proteins. United States: N. p., 2006. Web.
Qinhong, Cao, Department of Breast and Endocrine Surgery, Jiangsu Province Hospital, First Affiliated Hospital of Nanjing Medical University, Guang Zhou Road 300, Nanjing, Jiangsu Province, Jie, Chen, Li, Zhu, Yun, Liu, Zuomin, Zhou, Jiahao, Sha, Shui, Wang, & Jianmin, Li. A testis-specific and testis developmentally regulated tumor protein D52 (TPD52)-like protein TPD52L3/hD55 interacts with TPD52 family proteins. United States.
Qinhong, Cao, Department of Breast and Endocrine Surgery, Jiangsu Province Hospital, First Affiliated Hospital of Nanjing Medical University, Guang Zhou Road 300, Nanjing, Jiangsu Province, Jie, Chen, Li, Zhu, Yun, Liu, Zuomin, Zhou, Jiahao, Sha, Shui, Wang, and Jianmin, Li. 2006. "A testis-specific and testis developmentally regulated tumor protein D52 (TPD52)-like protein TPD52L3/hD55 interacts with TPD52 family proteins". United States.
@article{osti_20798990,
title = {A testis-specific and testis developmentally regulated tumor protein D52 (TPD52)-like protein TPD52L3/hD55 interacts with TPD52 family proteins},
author = {Qinhong, Cao and Department of Breast and Endocrine Surgery, Jiangsu Province Hospital, First Affiliated Hospital of Nanjing Medical University, Guang Zhou Road 300, Nanjing, Jiangsu Province and Jie, Chen and Li, Zhu and Yun, Liu and Zuomin, Zhou and Jiahao, Sha and Shui, Wang and Jianmin, Li},
abstractNote = {Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast cancer. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. To date, three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. Herein, we identified a novel TPD52-like sequence (TPD52L3, or hD55) in human testis using cDNA microarray. Sequence analysis of the deduced protein suggests that hD55 contains a coiled-coil motif and is highly conserved compared with other TPD52-like sequences. Yeast two-hybrid and GST pull-down assays revealed that hD55 interacts with hD52, hD53, hD54, and itself. cDNA microarray detection found that hD55 was expressed at 5.6-fold higher levels in adult testis than in fetal testis. Additionally, the expression profile shows that hD55 is testis-specific, indicating a potential role for hD55 in testis development and spermatogenesis.},
doi = {},
url = {https://www.osti.gov/biblio/20798990}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 344,
place = {United States},
year = {Fri Jun 09 00:00:00 EDT 2006},
month = {Fri Jun 09 00:00:00 EDT 2006}
}