Ubiquinone accumulates in the mitochondria of yeast mutated in the ubiquinone binding protein, Qcr8p
- School of Biological Sciences, University of Texas at Austin, Austin, TX 78712 (United States)
- Nutritional Sciences, University of Texas at Austin, Austin, TX 78712 (United States)
- Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056 (United States)
In Saccharomyces cerevisiae, the trans-membrane helix of Qcr8p, the ubiquinone binding protein of complex III, contributes to the Q binding site. In wild-type cells, residue 62 of the helix is non-polar (proline). Substitution of proline 62 with a polar, uncharged residue does not impair the ability of the cells to respire, complex III assembly is unaffected, ubiquinone occupancy of the Q binding site is unchanged, and mitochondrial ubiquinone levels are in the wild-type range. Substitution with a +1 charged residue is associated with partial respiratory competence, impaired complex III assembly, and loss of cytochrome b. Although ubiquinone occupancy of the Q binding site is similar to wild-type, total mitochondrial ubiquinone doubled in these mutants. Mutants with a +2 charged substitution at position 62 are unable to respire. These results suggest that the accumulation of ubiquinone in the mitochondria may be a compensatory mechanism for impaired electron transport at cytochrome b.
- OSTI ID:
- 20798971
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 344, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2006.03.110; PII: S0006-291X(06)00658-9; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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