{beta}-Catenin control of T-cell transcription factor 4 (Tcf4) importation from the cytoplasm to the nucleus contributes to Tcf4-mediated transcription in 293 cells

Hsu, H -T; Liu, P -C; Ku, S -Y; Jung, K -C; Hong, Y -R; Chinghai, Kao; Chihuei, Wang

doi:10.1016/j.bbrc.2006.02.193

Title: {beta}-Catenin control of T-cell transcription factor 4 (Tcf4) importation from the cytoplasm to the nucleus contributes to Tcf4-mediated transcription in 293 cells

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Abstract

{beta}-Catenin has essential roles in morphogenesis and human cancer, both as a subunit of adhesive complexes in the cell membrane and as a transcriptional coactivator in the Wnt signaling pathway. In addition, {beta}-catenin also has the ability to transport lymphoid enhancer binding factor-1 into the nucleus. In this study, we examined a constitutive active mutation, {beta}-catenin (T41A, S45A), for its potential as a nuclear import receptor for T-cell transcription factor 4 in 293 cells. Immunoblot analysis demonstrated that this constitutive active form of {beta}-catenin increased the amount of Tcf4 in the nucleus about 4-5-fold compared to controls. However, the overall expression of Tcf4 remained the same with or without over-expression of {beta}-catenin (T41A, S45A). T-cell transcription factor 4 reporter gene and electrophoretic mobility shift assay further indicated that the increase in Tcf4 in the nucleus was consistent with its accrued DNA binding capacity and transcription activity. Microscopic immunofluorescence examination showed that Tcf4 was mainly located in the cytoplasm and transported into the nucleus, without or with over-expression of {beta}-catenin (T41A, S45A), respectively. Our results suggest that {beta}-catenin might be a major factor regulating the import of Tcf4 from the cytoplasm into the nucleus, consequently controlling its transcription activity.

Authors:

Hsu, H -T ^[1]; Liu, P -C ^[1]; Ku, S -Y ^[1]; Jung, K -C ^[1]; Hong, Y -R ^[2]; Chinghai, Kao ^[3]; Chihuei, Wang ^[1]

Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan (China)
Department of Biochemistry, Kaohsiung Medical University, Kaohsiung 807, Taiwan (China)
Department of Urology, University of Indiana, Indianapolis, IN 46202 (United States)

Publication Date:: Fri May 12 00:00:00 EDT 2006

OSTI Identifier:: 20798946

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 343; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2006.02.193; PII: S0006-291X(06)00458-X; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; CELL MEMBRANES; CELL NUCLEI; CYTOPLASM; GENES; MORPHOGENESIS; MUTATIONS; NEOPLASMS; RECEPTORS; TRANSCRIPTION; TRANSCRIPTION FACTORS

Citation Formats


                    Hsu, H -T, Liu, P -C, Ku, S -Y, Jung, K -C, Hong, Y -R, Chinghai, Kao, and Chihuei, Wang. {beta}-Catenin control of T-cell transcription factor 4 (Tcf4) importation from the cytoplasm to the nucleus contributes to Tcf4-mediated transcription in 293 cells.  United States: N. p., 2006. 
        Web.  doi:10.1016/j.bbrc.2006.02.193.

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                    Hsu, H -T, Liu, P -C, Ku, S -Y, Jung, K -C, Hong, Y -R, Chinghai, Kao, & Chihuei, Wang. {beta}-Catenin control of T-cell transcription factor 4 (Tcf4) importation from the cytoplasm to the nucleus contributes to Tcf4-mediated transcription in 293 cells.  United States.  https://doi.org/10.1016/j.bbrc.2006.02.193

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                    Hsu, H -T, Liu, P -C, Ku, S -Y, Jung, K -C, Hong, Y -R, Chinghai, Kao, and Chihuei, Wang. 2006.  
        "{beta}-Catenin control of T-cell transcription factor 4 (Tcf4) importation from the cytoplasm to the nucleus contributes to Tcf4-mediated transcription in 293 cells".  United States.  https://doi.org/10.1016/j.bbrc.2006.02.193.

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@article{osti_20798946,

  title        = {{beta}-Catenin control of T-cell transcription factor 4 (Tcf4) importation from the cytoplasm to the nucleus contributes to Tcf4-mediated transcription in 293 cells},

  author       = {Hsu, H -T and Liu, P -C and Ku, S -Y and Jung, K -C and Hong, Y -R and Chinghai, Kao and Chihuei, Wang},

  abstractNote = {{beta}-Catenin has essential roles in morphogenesis and human cancer, both as a subunit of adhesive complexes in the cell membrane and as a transcriptional coactivator in the Wnt signaling pathway. In addition, {beta}-catenin also has the ability to transport lymphoid enhancer binding factor-1 into the nucleus. In this study, we examined a constitutive active mutation, {beta}-catenin (T41A, S45A), for its potential as a nuclear import receptor for T-cell transcription factor 4 in 293 cells. Immunoblot analysis demonstrated that this constitutive active form of {beta}-catenin increased the amount of Tcf4 in the nucleus about 4-5-fold compared to controls. However, the overall expression of Tcf4 remained the same with or without over-expression of {beta}-catenin (T41A, S45A). T-cell transcription factor 4 reporter gene and electrophoretic mobility shift assay further indicated that the increase in Tcf4 in the nucleus was consistent with its accrued DNA binding capacity and transcription activity. Microscopic immunofluorescence examination showed that Tcf4 was mainly located in the cytoplasm and transported into the nucleus, without or with over-expression of {beta}-catenin (T41A, S45A), respectively. Our results suggest that {beta}-catenin might be a major factor regulating the import of Tcf4 from the cytoplasm into the nucleus, consequently controlling its transcription activity.},

  doi          = {10.1016/j.bbrc.2006.02.193},

  url          = {https://www.osti.gov/biblio/20798946},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 3,

  volume       = 343,

  place        = {United States},

  year         = {Fri May 12 00:00:00 EDT 2006},

  month        = {Fri May 12 00:00:00 EDT 2006}

}

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