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Title: F F{sub 1}-ATPase as biosensor to detect single virus

Abstract

F F{sub 1}-ATPase within chromatophore was constructed as a biosensor (immuno-rotary biosensor) for the purpose of capturing single virus. Capture of virus was based on antibody-antigen reaction. The detection of virus based on proton flux change driven by ATP-synthesis of F F{sub 1}-ATPase, which was indicated by F1300, was directly observed by a fluorescence microscope. The results demonstrate that the biosensor loading of virus particles has remarkable signal-to-noise ratio (3.8:1) compared to its control at single molecular level, and will be convenient, quick, and even super-sensitive for detecting virus particles.

Authors:
 [1];  [2];  [1];  [3];  [1];  [4]
  1. The National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 (China)
  2. (China)
  3. The National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 (China). E-mail: yuejc@sun5.ibp.ac.cn
  4. The Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049 (China)
Publication Date:
OSTI Identifier:
20798910
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 342; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2006.02.103; PII: S0006-291X(06)00357-3; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; ANTIGENS; ATP; ATP-ASE; BIOSYNTHESIS; FLUORESCENCE; INFLUENZA VIRUSES; MICROSCOPES; SIGNAL-TO-NOISE RATIO

Citation Formats

Liu, XiaoLong, The Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, Zhang, Yun, Yue, JiaChang, Jiang, PeiDong, and Zhang, ZhenXi. F F{sub 1}-ATPase as biosensor to detect single virus. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2006.02.103.
Liu, XiaoLong, The Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, Zhang, Yun, Yue, JiaChang, Jiang, PeiDong, & Zhang, ZhenXi. F F{sub 1}-ATPase as biosensor to detect single virus. United States. doi:10.1016/j.bbrc.2006.02.103.
Liu, XiaoLong, The Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, Zhang, Yun, Yue, JiaChang, Jiang, PeiDong, and Zhang, ZhenXi. Fri . "F F{sub 1}-ATPase as biosensor to detect single virus". United States. doi:10.1016/j.bbrc.2006.02.103.
@article{osti_20798910,
title = {F F{sub 1}-ATPase as biosensor to detect single virus},
author = {Liu, XiaoLong and The Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049 and Zhang, Yun and Yue, JiaChang and Jiang, PeiDong and Zhang, ZhenXi},
abstractNote = {F F{sub 1}-ATPase within chromatophore was constructed as a biosensor (immuno-rotary biosensor) for the purpose of capturing single virus. Capture of virus was based on antibody-antigen reaction. The detection of virus based on proton flux change driven by ATP-synthesis of F F{sub 1}-ATPase, which was indicated by F1300, was directly observed by a fluorescence microscope. The results demonstrate that the biosensor loading of virus particles has remarkable signal-to-noise ratio (3.8:1) compared to its control at single molecular level, and will be convenient, quick, and even super-sensitive for detecting virus particles.},
doi = {10.1016/j.bbrc.2006.02.103},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 342,
place = {United States},
year = {Fri Apr 21 00:00:00 EDT 2006},
month = {Fri Apr 21 00:00:00 EDT 2006}
}